siRNA-mediated knockdown of sperm-associated antigen 11a (Spag11a) mRNA in epididymal primary epithelial cells affects proliferation: a transcriptome analyses

Kumari, Sangeeta; Yenugu, Suresh

doi:10.1007/s00441-019-03107-6

Cited by 4 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the meantime, the present results revealed that the immunolabeling of SPAG11A and AR were mainly located in the principal cell in the caput epididymis. Consistently, the expression abundance of SPAG11A in epididymis of mouse and rat also showed region-specific model, 14,34 and the secretion of SPAG11A in the lumen was proved to be from the principal cells as well. Furthermore, similar to Pujianto's results, 13 we found that SPAG11A was present in the sperms in the lumen of epididymis, and the immunostaining of SPAG11A in the corpus and cauda epididymis was higher than these in the caput epididymis.…”

Section: Discussionsupporting

confidence: 54%

“…12 Previous study found that these proteins have an inclination of region-and cell-specific expression in the epithelium of the postnatal epididymis, 13 and their secretion into the luminal microenvironment and binding to spermatozoa when they transport along the epididymis, suggesting that they were involved in male reproductive function. 14 SPAG11A is a member of the β-defensin protein family exclusively expressed in the epididymis. 15 As a secreted protein in the male accessory sex gland, SPAG11A has been shown to play essential roles in sperm maturation and fertility in different species, such us rats, mice and humans.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (<em>Citellus dauricus</em> Brandt)

Zhang

Liu

et al. 2020

Eur J Histochem

View full text Add to dashboard Cite

Sperm-associated antigen 11A (SPAG11A) is expressed exclusively in the epididymis, which can specifically regulate sperm maturation. The aim of this study was to investigate the seasonal expressions of beta-defensin (SPAG11A) and androgen receptor (AR) in the epididymis of the wild ground squirrels (Citellus dauricus Brandt). Morphologically, the results showed that epididymis length and weight in the breeding season were significantly higher than those of the non-breeding season. Histologically, the results revealed that enlarged lumen diameters, thickened epithelial and abundant sperm in the breeding season while reduced lumen diameters and epithelial with no sperm in the non-breeding season. SPAG11A was intensely expressed in cytoplasm and nucleus of epithelial cells in the breeding season, and weaker staining in the non-breeding season. The immunostaining of AR was only presented in nucleus of smooth muscle cells and epithelial cells in the whole epididymis with stronger staining in the breeding season. The results of real-time quantitative PCR also showed that the mRNA levels of SPAG11A and AR in the epididymis during the breeding season were significantly higher than those of the non-breeding season. In addition, the levels of testosterone (T), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the serum were higher during the breeding season. Taken together, these results suggested that SPAG11A might play an important role to regulate seasonal changes in epididymal function of the wild ground squirrels.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (<em>Citellus dauricus</em> Brandt)

Zhang

Liu

et al. 2020

Eur J Histochem

View full text Add to dashboard Cite

show abstract

“…To synthesize cDNA, 1 µg of total mRNA was used based on a commercially available protocol of BioFact cDNA synthesis kit (Daejeon, South Korea). 44 Amplification and alternation of target genes were performed by StepOne TM Real-Time PCR System instrument (Applied Biosystems, USA) with SYBR Green detection system. Suitable primers were designed by NCBI primer blast ( ), as mentioned in Table 3 .…”

Section: Methodsmentioning

confidence: 99%

Ferroptosis as a Potential Cell Death Mechanism against Cisplatin-Resistant Lung Cancer Cell Line

et al. 2021

View full text Add to dashboard Cite

Purpose: Drug resistance is a challenging issue in cancer chemotherapy. Cell death induction is one of the main strategies to overcome chemotherapy resistance. Notably, ferroptosis has been considered a critical cell death mechanism in recent years. Accordingly, in this study, the different cell death strategies focused on ferroptosis have been utilized to overcome cisplatin resistance in an in vitro lung cancer model. Methods: The physiological functions of Akt1 and GPX4, as critical targets for ferroptosis and apoptosis induction, were suppressed by siRNA or antagonistic agents in resistant A549 cells. Afterward, the interventions' impacts on cell viability, reactive oxygen species (ROS) amount were analyzed by flow cytometry. Moreover, the alteration in the relevant gene and protein expression levels were quantified using Real-time PCR and western blot methods. Results: The result showed that the treatment with Akt1 siRNA reversed the cisplatin resistance in the A549 cell line through the induction of apoptosis. Likewise, the combination treatment of the GPX4 siRNA or FIN56 as Ferroptosis inducers alongside cisplatin elevated ROS's cellular level, reduced the cellular antioxidant genes level, and increased the cisplatin cytotoxic effect. Conclusion: In conclusion, our study indicated that ferroptosis induction can be considered a promising cell death strategy in cisplatin-resistant cancer cells.

show abstract

“…We recently reported that overexpression of SPAG11A in immortalized epididymal epithelial cell lines resulted in inhibition of proliferation. 21 On the other hand, small interfering RNA (siRNA)-mediated silencing of Spag11a messenger RNA (mRNA) in isolated epididymal primary epithelial cells resulted in promotion of proliferation. 21 In our animal studies, the ablation of SPAG11A protein by active immunization resulted in the vulnerability of epididymis to a low dose carcinogen.…”

mentioning

confidence: 99%

“…21 On the other hand, small interfering RNA (siRNA)-mediated silencing of Spag11a messenger RNA (mRNA) in isolated epididymal primary epithelial cells resulted in promotion of proliferation. 21 In our animal studies, the ablation of SPAG11A protein by active immunization resulted in the vulnerability of epididymis to a low dose carcinogen. 22 These studies clearly indicated that SPAG11A plays a crucial part in regulating the cell proliferation and thus could be one of the mechanisms that defend the epididymis from chemical carcinogenesis.…”

mentioning

confidence: 99%

Effect of Spag11a gene knockout on the epididymis in mice: A histopathological and molecular analyses

Aisha,

Sangeeta,

Yenugu

2024

Cell Biochemistry & Function

Self Cite

View full text Add to dashboard Cite

The sperm‐associated antigen 11a (Spag11a) gene is exclusively expressed in the caput epididymis. Our previous studies demonstrated that small interfering RNA (siRNA)‐mediated ablation of this gene resulted in increased proliferation of epididymal epithelial cells. Further, active immunization‐mediated ablation of SPAG11A protein increased the susceptibility of male reproductive tract tissues to diethylnitrosamine (DEN)‐induced tumorigenesis. In this study, we report that the caput epididymis of Spag11a knockout mice displayed hyperplasia and inflammation, while the caput epididymis of wild‐type mice exhibited normal anatomical structure. Global transcriptome analyses in the caput epididymis of knockout mice indicated differential expression of genes involved in a variety of cellular processes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses suggested that the absence of Spag11a may activate microRNAs associated with cancer, chemical carcinogenesis‐receptor activation, and chemical carcinogenesis‐DNA adducts pathways; which may contribute to the promotion of tumorigenesis in the epididymis. The susceptibility of caput epididymis to chemically induced carcinogenesis in Spag11a knockout mice was analyzed. Histological analyses indicated that while the epididymis of wild‐type mice did not show any signs of tumorigenesis, knockout mice displayed hyperplasia, anaplasia, dysplasia, neoplasia, and inflammation in the caput epididymis. Our results provide concrete evidence that deletion of Spag11a induces histopathological and molecular changes that contribute to tumorigenesis. It is possible that the expression of Spag11a gene could be one of the reasons for the rarity of epididymal cancers. The involvement of an epididymal gene in tumorigenesis is being demonstrated for the first time.

show abstract

siRNA-mediated knockdown of sperm-associated antigen 11a (Spag11a) mRNA in epididymal primary epithelial cells affects proliferation: a transcriptome analyses

Cited by 4 publications

References 48 publications

Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (<em>Citellus dauricus</em> Brandt)

Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (<em>Citellus dauricus</em> Brandt)

Ferroptosis as a Potential Cell Death Mechanism against Cisplatin-Resistant Lung Cancer Cell Line

Effect of Spag11a gene knockout on the epididymis in mice: A histopathological and molecular analyses

Contact Info

Product

Resources

About