SIROF stabilized PEDOT/PSS allows biocompatible and reversible direct current stimulation capable of driving electrotaxis in cells

Leal, José; Jedrusik, Nicole; Shaner, Sebastian; Boehler, Christian; Asplund, Maria

doi:10.1016/j.biomaterials.2021.120949

Cited by 32 publications

(58 citation statements)

References 69 publications

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“…[ 83 ] In addition, CP coatings offer the possibility to work in typical ranges not covered by conventional electrodes, that is, DC and low frequency regimes. [ 214 ] Shifting the focus away from coatings, CP‐based organic transistors are likely the most reliable approach to make the leap toward high SNR recording in vivo, due to their inherent in situ signal amplification signal. The possibility of recording LFPs, single‐ and multi‐unit activity from the brain surface with the same accuracy of conventional intracortical probes was demonstrated in rats to date.…”

Section: Discussionmentioning

confidence: 99%

“…However, as the material is semi‐permeable, the internal surface area of the material contributes with a much larger ionic pseudocapacitance contributed by the bulk of the CP film. [ 213 , 214 , 215 ] This bulk may inject or absorb small ions from/to the electrolyte in response to a shift in polarity. Thus, this reaction is not specific to certain reactants but can involve any ions available at the interface that are small enough to penetrate the film.…”

Section: In Vivo Stimulation With Pedot‐based Neural Interfacesmentioning

confidence: 99%

“…The work on DC stimulation using IrOx:PEDOT/PSS was furthermore extended by Leal et al demonstrating DCS of cells over several hours. [214] As the generation of the direct current only involved electrochemically reversible electrostatic interaction with small ions, the electrodes could be re-charged with intermittent reversed pulses, offering a completely new concept for biocompatible DCS. In summary CPs do not only outperform most metals with conventional stimulation parameters and charge injection measures, but furthermore can cover a parametric range that typically is not possible to achieve with metal electrodes, namely extremely low frequency and DC.…”

Section: In Vivo Stimulation With Pedot-based Neural Interfacesmentioning

confidence: 99%

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Poly(3,4‐ethylenedioxythiophene)‐Based Neural Interfaces for Recording and Stimulation: Fundamental Aspects and In Vivo Applications

et al. 2022

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Next‐generation neural interfaces for bidirectional communication with the central nervous system aim to achieve the intimate integration with the neural tissue with minimal neuroinflammatory response, high spatio‐temporal resolution, very high sensitivity, and readout stability. The design and manufacturing of devices for low power/low noise neural recording and safe and energy‐efficient stimulation that are, at the same time, conformable to the brain, with matched mechanical properties and biocompatibility, is a convergence area of research where neuroscientists, materials scientists, and nanotechnologists operate synergically. The biotic–abiotic neural interface, however, remains a formidable challenge that prompts for new materials platforms and innovation in device layouts. Conductive polymers (CP) are attractive materials to be interfaced with the neural tissue and to be used as sensing/stimulating electrodes because of their mixed ionic‐electronic conductivity, their low contact impedance, high charge storage capacitance, chemical versatility, and biocompatibility. This manuscript reviews the state‐of‐the‐art of poly(3,4‐ethylenedioxythiophene)‐based neural interfaces for extracellular recording and stimulation, focusing on those technological approaches that are successfully demonstrated in vivo. The aim is to highlight the most reliable and ready‐for‐clinical‐use solutions, in terms of materials technology and recording performance, other than spot major limitations and identify future trends in this field.

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Section: Discussionmentioning

confidence: 99%

Section: In Vivo Stimulation With Pedot‐based Neural Interfacesmentioning

confidence: 99%

Section: In Vivo Stimulation With Pedot-based Neural Interfacesmentioning

confidence: 99%

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Poly(3,4‐ethylenedioxythiophene)‐Based Neural Interfaces for Recording and Stimulation: Fundamental Aspects and In Vivo Applications

et al. 2022

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“…Direct current (DC) compatible electrode materials are a key ingredient for EF stimulation in vitro, and will furthermore be essential for clinical translation. 28,29 Here we show that electrodes based on a combination of laser-induced graphene (LIG) and PEDOT:PSS hydrogel, integrated within the platform, were capable of sustaining DC stimulation over hours. This is not only important for the in vitro application, but likewise a prerequisite for subsequent clinical translation of the concept.…”

Section: Introductionmentioning

confidence: 94%

“…Additionally, the disconnected and polarized anode can passively recharge with ions from solution while the other anode is delivering charge. 29 Using finite element analysis (FEA) of the three-dimensional microfluidic device, we identified the input current needed to achieve ≃ 200 mV mm −1 at the center of the scratch, which has been shown to be an optimal EF strength for in vitro keratinocyte electrotaxis. [36][37][38] As validated by the FEA model, the geometric confinement around the monolayer of scratched cells provided by the microchannel allows for precise control of the field distribution (Fig.…”

Section: /15mentioning

confidence: 99%

Bioelectronic microfluidic wound healing

Shaner

Savelyeva

Kvartuh

et al. 2022

Preprint

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This work delves into the impact of direct current (DC) stimulation on both healthy and diabetic in vitro wound healing models of keratinocytes, the most prevalent cell type of the skin. The augmentation of non-metal electrode materials and prudent microfluidic design allowed for a platform to study the effects of different sustained (12 hours DC) electric field configurations on wound closure dynamics. We found that electric guidance cues (≃ 200 mV/mm) enhance wound closure rate by nearly 3X for both healthy and diabetic-like keratinocyte sheets, compared to their respective controls. The motility-inhibited keratinocytes regained wound closure rates with stimulation (increase from 1.0 to 2.8 %/hr) comparable to healthy non-stimulated keratinocyte collectives (3.5 %/hr). Our results bring hope that electrical stimulation is a viable pathway to accelerate wound repair.

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Decellularized Biohybrid Nerve Promotes Motor Axon Projections

Mehta,

Zhang,

Xie

et al. 2024

Adv Healthcare Materials

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Developing nerve grafts with intact mesostructures, superior conductivity, minimal immunogenicity, and improved tissue integration is essential for the treatment and restoration of neurological dysfunctions. A key factor is promoting directed axon growth into the grafts. To achieve this, biohybrid nerves are developed using decellularized rat sciatic nerve modified by in situ polymerization of poly(3,4‐ethylenedioxythiophene) (PEDOT). Nine biohybrid nerves are compared with varying polymerization conditions and cycles, selecting the best candidate through material characterization. These results show that a 1:1 ratio of FeCl3 oxidant to ethylenedioxythiophene (EDOT) monomer, cycled twice, provides superior conductivity (>0.2 mS cm−1), mechanical alignment, intact mesostructures, and high compatibility with cells and blood. To test the biohybrid nerve's effectiveness in promoting motor axon growth, human Spinal Cord Spheroids (hSCSs) derived from HUES 3 Hb9:GFP cells are used, with motor axons labeled with green fluorescent protein (GFP). Seeding hSCS onto one end of the conduit allows motor axon outgrowth into the biohybrid nerve. The construct effectively promotes directed motor axon growth, which improves significantly after seeding the grafts with Schwann cells. This study presents a promising approach for reconstructing axonal tracts in humans.

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SIROF stabilized PEDOT/PSS allows biocompatible and reversible direct current stimulation capable of driving electrotaxis in cells

Cited by 32 publications

References 69 publications

Poly(3,4‐ethylenedioxythiophene)‐Based Neural Interfaces for Recording and Stimulation: Fundamental Aspects and In Vivo Applications

Poly(3,4‐ethylenedioxythiophene)‐Based Neural Interfaces for Recording and Stimulation: Fundamental Aspects and In Vivo Applications

Bioelectronic microfluidic wound healing

Decellularized Biohybrid Nerve Promotes Motor Axon Projections

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