Sirolimus Encapsulated Liposomes for Cancer Therapy: Physicochemical and Mechanical Characterization of Sirolimus Distribution within Liposome Bilayers

Onyesom, Ichioma; Lamprou, Dimitrios A.; Sygellou, Lamprini; Owusu-Ware, Samuel K.; Antonijević, Milan D.; Chowdhry, Babur Z.; Douroumis, Dennis

doi:10.1021/mp400362v

Cited by 40 publications

(25 citation statements)

References 38 publications

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“…The major component appearing at 283.18 eV corresponds to carbon only bound to carbon and hydrogen (C−C/C−H). The peak at 284.73 eV is attributed to carbon making a single bond with oxygen or nitrogen (C−O/C−N) and the peak at higher binding energy (286.88 eV) includes carbon making one double bond and one single bond with oxygen (O=C‐OR) …”

Section: Resultsmentioning

confidence: 99%

Liposome-Permeability Templating of Gadolinium Hydroxide Nanostructures

Municoy

Bellino

2016

ChemistrySelect

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With the growing attention in building novel nanostructures, there is a need for general approaches to controlling the sizes and shapes in precipitation processes. Here, precipitated gadolinium hydroxides (Gd(OH) 3 ) were made in the form of hollow spheres by control of the liposome permeability. Addition of Gd(NO 3 ) 3 to the acidic solution containing the liposomes entrapping OH À creates a self-precipitating template in the precipitation process. The control-formation of these nano-structures takes place through a liposome-membrane phase change. Hybrid nanocapsules are obtained when entrapped hydroxyl ions (OH À ) permeates from the liposome and shell material precipitates by forming Gd(OH) 3 . The morphology of the final product is molded on the original liposome. This synthesis strategy might have implications in significant applications, such as drug delivery systems, and in mineralization, where many processes are also membrane-assisted.

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Section: Resultsmentioning

confidence: 99%

Liposome-Permeability Templating of Gadolinium Hydroxide Nanostructures

Municoy

Bellino

2016

ChemistrySelect

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“…In AFM detection, the most common problem is the collapse of lipids, however, despite that Kanno et al [40] demonstrated that the diameter acquired by AFM measurements can be compared with the size obtained using DLS analysis evaluating the differences. According to DLS, AFM reveals a difference in terms of reduction of particle size for lipids modified with cholesterol [41]. Furthermore, the formulation with ratio 70-30 % for each lipid analysed shows a small standard deviation, suggesting a well-assembled structure.…”

Section: Particle Size Measurementsmentioning

confidence: 94%

Influence of cholesterol on liposome stability and on in vitro drug release

Briuglia

Rotella

McFarlane

et al. 2015

Drug Deliv. and Transl. Res.

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542

268

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Cholesterol plays a strategic role in liposome composition; however, the quantity used to achieve an appropriate formulation has not been yet clarified. Therefore, by screening arrangement of lipids and cholesterol ratio, the main aim of this study is to investigate the most suitable amount of cholesterol in lipids in order to prepare stable and controlled drug release vehicles. For the preparation of liposomes, DMPC, DPPC and DSPC phospholipids were used and combined with different molar ratios of cholesterol (e.g. 100, 80-20, 70-30, 60-40 and 50-50%). Stability studies were conducted by storing the formulations at 37 and 50 °C for 30 days and by analysing them by AFM, DLS and FT-IR. By detecting the two most stable formulations from the stability results, drug encapsulation and in vitro release studies in PBS were performed by encapsulating atenolol and quinine. The release results were validated using a simulation model to ensure the reliability and suitable interpretation of the data. The generated model showed a good correlation between the prediction and the in vitro obtained results. By using 70:30% ratio (known in literature as 2:1), it is possible to reach the most stable formulation to guarantee a controlled and reproducible release for drugs with different physicochemical characteristics and pharmaceutical applications.

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“…Briefly, a 0.5 mL of MLV liposomal suspension was spun at 25,000 g for 90 min at 4 °C; after having verified by the Stewart assay [ 70 ] (data reported in the Supporting Information ) the absence of phospholipids in every supernatant, the concentration of free EGCG was determined spectrophotometrically at 280 nm using a UV-visible spectrophotometer (Multi-Mode Sinergy, HT BioTek, Winooski, VT, USA). The EGCG encapsulating efficiency (EE) was calculated as percentage according to Equation (1) [ 71 , 72 ]: EE% = 100 × [the amount added − the amount unentrapped]/[the amount added] …”

Section: Methodsmentioning

confidence: 99%

Liposomal Formulations for an Efficient Encapsulation of Epigallocatechin-3-Gallate: An In-Silico/Experimental Approach

et al. 2018

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As a part of research project aimed to optimize antioxidant delivery, here we studied the influence of both salts and lipid matrix composition on the interaction of epigallocatechin-3-gallate (EGCG) with bilayer leaflets. Thus, we combined in silico and experimental methods to study the ability of neutral and anionic vesicles to encapsulate EGCG in the presence of Ca2+ and Mg2+ divalent salts. Experimental and in silico results show a very high correlation, thus confirming the efficiency of the developed methodology. In particular, we found out that the presence of calcium ions hinders the insertion of EGCG in the liposome bilayer in both neutral and anionic systems. On the contrary, the presence of MgCl2 improves the insertion degree of EGCG molecules respect to the liposomes without divalent salts. The best and most efficient salt concentration is that corresponding to a 5:1 molar ratio between Mg2+ and EGCG, in both neutral and anionic vesicles. Concerning the lipid matrix composition, the anionic one results in better promotion of the catechin insertion within the bilayer since experimentally we achieved 100% EGCG encapsulation in the lipid carrier in the presence of a 5:1 molar ratio of magnesium. Thus, the combination of this anionic liposomal formulation with magnesium chloride, avoids time-consuming separation steps of unentrapped active principle and appears particularly suitable for EGCG delivery applications.

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Sirolimus Encapsulated Liposomes for Cancer Therapy: Physicochemical and Mechanical Characterization of Sirolimus Distribution within Liposome Bilayers

Cited by 40 publications

References 38 publications

Liposome-Permeability Templating of Gadolinium Hydroxide Nanostructures

Liposome-Permeability Templating of Gadolinium Hydroxide Nanostructures

Influence of cholesterol on liposome stability and on in vitro drug release

Liposomal Formulations for an Efficient Encapsulation of Epigallocatechin-3-Gallate: An In-Silico/Experimental Approach

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