SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease

Chan, Shih Hung; Hung, Ching Hsia; Shih, Jhih Yuan; Chu, Pei Ming; Cheng, Yung Hsin; Lin, Huei Chen; Tsai, Kun Ling

doi:10.1016/j.redox.2017.05.027

Cited by 76 publications

(55 citation statements)

References 43 publications

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“…Sirtuin 1 (SIRT1) is a NAD+-dependent deacetylase important in regulating cell apoptosis (25). Studies have also indicated that SIRT1 plays critical roles in the regulation of inflammatory responses (26)(27)(28)(29). Notably, potential binding sites between SirT1 and mir-138-5p were detected in the present study via bioinformatics software analysis.…”

Section: Introductionmentioning

confidence: 61%

MicroRNA‑138‑5p regulates the development of spinal cord injury by targeting SIRT1

Chen

Qin

2020

Mol Med Report

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Micrornas (mirs) play an important role in the development and progression of spinal cord injury (Sci). The role of mir-138-5p in Sci was investigated in the present study. The anti-inflammatory effects of miR-138-5p and underlying mechanisms were investigated in an Sci rat model and in vitro model. reverse transcription-quantitative Pcr (rT-qPcr) was used to examine the expression of mir-138-5p in the Sci in vivo and in vitro models, as well as patients with Sci; it was found that miR-138-5p was significantly upregulated in Sci. Bioinformatics and dual-luciferase reporter assays were performed to predict and confirm the binding sites between mir-138-5p and the 3'untranslated region of sirtuin 1 (SirT1). Then, the expression of SirT1 was detected via rT-qPcr and western blotting, indicating downregulation of SIRT1 in SCI. PC12 cells were transfected with miR-138-5p inhibitor, inhibitor control or miR-138-5p inhibitor + SIRT1 small interfering RNA for 48 h, and then subjected to lipopolysaccharide (100 ng/ml) treatment for 4 h. Then, MTT assay, flow cytometry and eliSa experiments were performed to analyze cell viability, apoptosis, and the levels of tumor necrosis factor-α, interleukin (il)-1β and il-6. Findings suggested that downregulation of miR-138-5p increased PC12 cell viability, inhibited cell apoptosis and attenuated proinflammatory responses, which may result in amelioration of Sci. However, all these effects were reversed by SIRT1 knockdown. Finally, it was observed that miR-138-5p altered the related protein expression of the PTen/aKT pathway. These results indicated that miR-138-5p could regulate inflammatory responses and cell apoptosis in SCI models by modulating the PTEN/AKT signaling pathway via SirT1, thus playing an important role in the development of Sci. collectively, the present study demonstrated that miR-138-5p may be a novel therapeutic target for the treatment of Sci. Materials and methodsClinical samples. a total of 18 serum specimens from patients with Sci (age range, 27-58 years; 12 male patients and 6 female MicroRNA-138-5p regulates the development of spinal cord injury by targeting SIRT1 JincHuan cHen and ruJie Qin department of Spine Surgery, The First People's Hospital of lianyungang, lianyungang, Jiangsu 222000, P.r. china

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Section: Introductionmentioning

confidence: 61%

MicroRNA‑138‑5p regulates the development of spinal cord injury by targeting SIRT1

Chen

Qin

2020

Mol Med Report

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“…SirT1, an nad + -dependent deacetylase, is involved in apoptosis (21) and serves important roles in the regulation of inflammatory responses (22)(23)(24)(25). For example, SIRT1 protein levels are downregulated by IL1β/nFκB signaling in acetaminophen hepatotoxicity, resulting in inflammation and oxidative stress (23).…”

Section: Discussionmentioning

confidence: 99%

“…For example, SIRT1 protein levels are downregulated by IL1β/nFκB signaling in acetaminophen hepatotoxicity, resulting in inflammation and oxidative stress (23). The inhibition of SIRT1 leads to oxidative stress and inflammation in patients with coronary artery disease (24). Wu et al reported that SIRT1 is involved in the invasion and metastasis of human esophageal cancer cells by inducing epithelial mesenchymal transition through the regulation of Snail expression (43).…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑217 is involved in the progression of atherosclerosis through regulating inflammatory responses by targeting sirtuin 1

Zhang

Chen

et al. 2019

Mol Med Report

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Atherosclerosis is a chronic inflammatory disease, and it is a global clinical problem. The development of new and effective therapeutic targets for atherosclerosis is necessary. A number of microRNAs (miRNAs) have been demonstrated to serve a crucial role in atherosclerosis. However, the role of miRNA (miR)-217 in atherosclerosis remains unclear. Therefore, the aim of the present study was to investigate the role and mechanism of miR-217 in atherosclerosis. The level of miR-217 was detected in the blood of patients with atherosclerosis using reverse transcription-quantitative PCR. THP-1 acute monocytic leukemia cells were treated with oxidized low-density lipoprotein (ox-LDL) to develop an atherosclerotic cell model of macrophages. The relationship between miR-217 and sirtuin 1 (SIRT1) was determined by TargetScan and dual luciferase reporter assay. Cell apoptosis was measured by flow cytometry. Production of pro-inflammatory factors and triglyceride (TG) and total cholesterol (TC) levels were also determined. The results demonstrated that miR-217 was significantly upregulated in atherosclerosis. SIRT1 was demonstrated to be a direct target of miR-217 and was downregulated in atherosclerosis. Downregulation of miR-217 significantly inhibited ox-LDL-induced TG and TC level increase, cell apoptosis and the upregulation of the pro-inflammatory factors tumor necrosis factor α, interleukin (IL)-6 and IL-1β. Additionally, the SIRT1/AMP-activated protein kinase α/NF-κB pathway was at least partially involved in modulating the effects of miR-217 inhibition on THP-1 cells treated with ox-LDL. In addition, the effects of miR-217 downregulation on ox-LDL-treated THP-1 cells were eliminated by SIRT1 silencing. In conclusion, the results of the present study indicated that miR-217 downregulation may relieve atherosclerosis through the inhibition of macrophage apoptosis and inflammatory response by targeting SIRT1.

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“…Resveratrol downregulates oxidative stress and reduces inflammation in persons with chronic disease or traumatic injury (41)(42)(43). The effects of SIRT1 on oxidative stress are essential in suppressing inflammation.…”

Section: Discussionmentioning

confidence: 99%

Expression of silent information regulator 2 homolog 1 (SIRT1) in periapical granulomas

et al. 2018

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Silent information regulator 2 homolog 1 (SIRT1) inhibits oxidative injury and has anti-inflammatory effects. SIRT1 may be involved in healing of periapical periodontitis; however, SIRT1 expression in periapical periodontitis lesions has not been investigated. This study evaluated SIRT1 expression and a marker of oxidative stress-8-hydroxy-2'-deoxyguanosine (8-OHdG)-in periapical granulomas. First, we used real-time polymerase chain reaction to determine whether U-937 monocytes express SIRT1. U-937 cells treated with the SIRT1 activator resveratrol exhibited the highest SIRT1 mRNA level after 6-h incubation. By contrast, treating cells with the SIRT1 inhibitor sirtinol returned SIRT1 expression level to that of the control. In addition, immunocytochemical analysis using cytospin specimens showed that U-937 cells co-expressed SIRT1 and Ki-67. Dual-color immunofluorescence imaging showed that round cells in periapical granulomas co-expressed SIRT1 and 8-OHdG; however, neither was expressed in healthy gingival tissues. The number of 8-OHdG-expressing cells was significantly greater than the number of SIRT1-expressing cells. Our findings suggest that macrophages express SIRT1 and that wound healing in periapical granulomas is enhanced by a SIRT1-mediated reduction in the level of oxidative stress.

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SIRT1 inhibition causes oxidative stress and inflammation in patients with coronary artery disease

Cited by 76 publications

References 43 publications

MicroRNA‑138‑5p regulates the development of spinal cord injury by targeting SIRT1

MicroRNA‑138‑5p regulates the development of spinal cord injury by targeting SIRT1

MicroRNA‑217 is involved in the progression of atherosclerosis through regulating inflammatory responses by targeting sirtuin 1

Expression of silent information regulator 2 homolog 1 (SIRT1) in periapical granulomas

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