SIRT1 inhibits Smad3 acetylation by reducing Lnc CRNDE transcription and inhibits BV2 cell overactivation to promote SCI neurological repair
Hongbo Chen,
Qiaofen Li,
Huimin Yu
et al.
Abstract:Methods
We established a BV2 cell activation model by in vitro lipopolysaccharide (LPS) treatment and a rat SCI model according to physical injury. We used HE staining, Luxol solid blue staining and Nissl staining to observe the spinal cord structure, RT‒qPCR to detect SIRT1 mRNA and Lnc CRNDE expression, western blotting to detect SIRT1 protein, BV2 cell activation protein marker (Iba-1) and autophagy-related protein (LC3; Beclin-1; P62) expression, immunoprecipitation reaction to detect the relationship betw… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.