SIRT1 Is the Target Gene for 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-Glucoside Alleviating the HUVEC Senescence

Abstract

This study aimed to explore the effects of 2,3,5,4'-tetrahydroxy-stilbene-2-O-b-dglucoside (TSG) on the senescence of human umbilical vein cells (HUVEC) induced by hydrogen peroxide (H 2 O 2) and to identify the potential targets mediating its protective action. HUVEC cells pre-treated with TSG for 24 h were exposed to H 2 O 2 treatment. TSG significantly decreased H 2 O 2-induced cellular senescence, as indicated by reduced senescence-associated b-galactosidase (SA-b-gal) positive staining, the proportion of cells in the G1 phase, cell apoptosis, p21, and plasminogen activator inhibitor-1 (PAI-1) expression. Moreover, TSG promoted Sirtuin 1 (SIRT1) expression. When SIRT1 was inhibited by EX527 or SIRT1 siRNA, the effect of TSG is diminished according to the increased proportion of cells in the G1 phase, cell apoptosis, p21, and PAI-1 expression. Overall, our study established TSG as an anti-senescence compound that exerts its protective action by regulating SIRT1 expression.

Apremilast prevents IL‑17‑induced cellular senescence in ATDC5 chondrocytes mediated by SIRT1

“…It has been reported that chondrocyte senescence could be mediated by IL-1β treatment via the p53/p21/pRb pathway, with the induction of caveolin 1 and activation of p38 mitogen-activated protein kinase (MAPK), resulting in cell senescence and apoptosis (32). Moreover, researchers have revealed that the expression levels of PAI-1 and p21 proteins have a positive correlation with the presence of SA-β-gal (2,33). PAI-1 is a member of the evolutionarily conserved serine protease inhibitor (SERPIN) family and is well known as a marker and mediator of senescence, revealed by its increased expression in senescent cells (34,35).…”