Sirt1/Nrf2 pathway is involved in oocyte aging by regulating Cyclin B1

Abstract

Nuclear factor erythroid 2-related factor 2 (Nrf2) is capable of inducing a variety of biological effects, and the regulation of the Nrf2 signaling pathway is closely related to longevity. To find out whether the nuclear factor erythroid 2-related factor 2 (Nrf2) is involved in oocyte aging or not which may cause reduced female fertility, a series of biological methods was applied, including oocyte collection and culture, micro injection, RNA interference, western blotting, immunofluorescence and confocal microscopy, and quantitative real-time PCR.Our data demonstrated that Nrf2 depletion disrupted oocyte maturation and spindle/chromosome organization by suppressing Cyclin B1 expression. Sirtuin 1 (Sirt1) depletion reduced Nrf2 expression, which indicated the existence of the Sirt1-Nrf2-Cyclin B1 signaling pathway in mouse oocytes. Additionally, immunoblotting results reflected a lower Nrf2 protein level in oocytes from aged mice, and maternal age-associated meiotic defects can be ameliorated through overexpression of Nrf2, which supported the hypothesis that decreased Nrf2 is an important factor contributing toward oocyte age-dependent deficits. Furthermore, we show that the expression of Nrf2 is related to female age in ovarian granular cells, suggesting that the decreased expression of Nrf2 may be related to the decline in the reproductive capacity of older women.

The Protective Effect of DiDang Tang Against AlCl3-Induced Oxidative Stress and Apoptosis in PC12 Cells Through the Activation of SIRT1-Mediated Akt/Nrf2/HO-1 Pathway

“…It has been reported that BDNF expression, Akt phosphorylation, and Nrf2 acetylation/deacetylation are mediated and critically controlled by SIRT1 (Pillai Vinodkumar et al, 2014;Ma et al, 2018). Based on these findings, we analyzed the effect of DDT on the expression of SIRT1 using western blot analysis.…”

“…Sirtuin 1 (SIRT1) plays protective roles against several neurodegenerative diseases including Alzheimer's, Parkinson's, and motor neuron diseases (Guarente, 1999). Many studies have indicated that brain derived neurotrophic factor (BDNF) expression, Akt phosphorylation, and nuclear factor E2 related factor 2 (Nrf2) acetylation/deacetylation are mediated and critically controlled by SIRT1 (Pillai Vinodkumar et al, 2014;Ma et al, 2018). Moreover, the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway plays a critical role in apoptosis (Zhu et al, 2016), which is important in maintaining mitochondrial integrity via the phosphorylation of proteins.…”

Salvianolic acid�B protects against sepsis‑induced liver injury via activation of SIRT1/PGC‑1α signaling

“…However, whether SalB has a protective effect against sepsis-induced liver injury remains unknown. Sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotide-dependent class III histone deacetylase, has been reported to play critical roles in various conditions, including oxidative stress, senescence and inflammation (14,15). Additionally, SIRT1 can activate peroxisome proliferator-activated receptor-γ co-activator 1α (PGC-1α), which is a key regulator in oxidative stress of the mitochondria (16).…”

ASB7 Is a Novel Regulator of Cytoskeletal Organization During Oocyte Maturation

“…to be responsible for the fertility loss of aged females (Ma et al, 2018;Yang et al, 2018;He et al, 2019;Nasheed Hamad Almohammed et al, 2020). Hence, dissection in greater depth of the possible interaction between ASB7 and these factors deserves further investigation.…”