Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9

Abstract

ObjectiveTo explore the role and mechanism of Sirt1 in protecting neural stem cells (NSCs) from apoptosis.Materials and methodsTransfection was used to overexpress Sirt1 in rat NSCs. The effect of Sirt1 overexpression on camptothecin-induced apoptosis of NSCs was evaluated. Western blotting was used to examine the expression of Sirt1, cleaved caspase-3, and acetylated histone 3K9.ResultsOverexpression of Sirt1 in NSCs decreased the cleavage of caspase-3 and acetylation of histone 3K9.ConclusionSirt1 may protect NSCs from apoptosis by decreasing the acetylation of histone 3 on K9.