Sirtuin1 (SIRT1) is involved in the anticancer effect of black raspberry anthocyanins in colorectal cancer

Chen, Lili; Li, Mei; Zhou, Hongrui; Liu, Yue; Pang, Wenqian; Ma, Teng; Niu, Chang; Yang, Zhe; Chang, Alan K.; Li, Xiaolong; Bi, Xiuli

doi:10.1007/s00394-022-02989-7

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…This study demonstrated that a BRB diet (4.1 g/kg) significantly reduced tumor multiplicity, emphasizing BRB’s role in preventing the progression of CRC. In line with previous in vitro experiments, these in vivo studies demonstrate BRB-mediated reduction in the anti-apoptotic protein Bcl-2 and increased expression of the apoptotic protein, Bax ( Figure 2 ) [ 53 ]. Similarly, Chen et al observed a significant reduction in tumor multiplicity in a chemically induced CRC (AOM/DSS) mouse model after dietary administration of 5% and 10% BRB [ 55 ].…”

Section: Black Raspberries and Cancersupporting

confidence: 90%

“…In vitro studies demonstrate an important role for BRBs in inhibiting CRC growth, and several mechanisms have been identified. One such study using human CRC cell lines SW480 and Caco2, demonstrated a growth inhibitory effect of a BRB extract, with mechanisms associated with the promotion of histone acetylation, upregulation of p65, and downregulation of IκB, pathways which are known to be important in CRC progression [ 52 , 53 , 54 ]. Similar inhibitory effects of BRB on cancer cell proliferation, migration, and colony formation in vitro were observed in HCT-116 and LoVo CRC cell lines [ 55 ].…”

Section: Black Raspberries and Cancermentioning

confidence: 99%

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Berries vs. Disease: Revenge of the Phytochemicals

Lamenza,

Upadhaya,

Roth

et al. 2024

Pharmaceuticals

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Secondary metabolites and phytochemicals in plant-based diets are known to possess properties that inhibit the development of several diseases including a variety of cancers of the aerodigestive tract. Berries are currently of high interest to researchers due to their high dietary source of phytochemicals. Black raspberries (BRB), Rubus occidentalis, are of special interest due to their rich and diverse composition of phytochemicals. In this review, we present the most up-to-date preclinical and clinical data involving berries and their phytochemicals in the chemoprevention of a variety of cancers and diseases. BRBs possess a variety of health benefits including anti-proliferative properties, anti-inflammatory activity, activation of pro-cell-death pathways, modulation of the immune response, microbiome modulation, reduction in oxidative stress, and many more. However, little has been done in both preclinical and clinical settings on the effects of BRB administration in combination with other cancer therapies currently available for patients. With the high potential for BRBs as chemopreventive agents, there is a need to investigate their potential in combination with other treatments to improve therapeutic efficacy.

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Section: Black Raspberries and Cancersupporting

confidence: 90%

Section: Black Raspberries and Cancermentioning

confidence: 99%

Berries vs. Disease: Revenge of the Phytochemicals

Lamenza,

Upadhaya,

Roth

et al. 2024

Pharmaceuticals

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“…Berries or their bioactive components may be a strong candidate. Berries such as black raspberries are recognized to have health benefits, and moderate intake of berries can prevent various human diseases, including diabetes, cardiovascular disease, and cancers [ 12 , 13 , 14 , 15 ]. In our previous study, the composition and function of the gut microbiota of healthy mice fed with black raspberries was characterized, and it was found that the gut microbiota composition and metabolic activity of black raspberry-fed mice was significantly different from that of the control group [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Berries, including raspberries, blueberries, strawberries, and cherries, are fruit with diverse health benefits, including anti-inflammatory, antioxidant, and anti-carcinogenic effects [ 12 , 13 , 14 , 15 ]. We previously reported that consumption of black raspberries was associated with the effective modulation of the gut microbiome [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Characterizing the Gut Microbial Metabolic Profile of Mice with the Administration of Berry-Derived Cyanidin-3-Glucoside

Zheng

Niu

et al. 2023

Metabolites

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Dietary modulation of the gut microbiota has recently received considerable attention. It is well established that consumption of berries confers a number of health benefits. We previously reported that a black raspberry (BRB)-rich diet effectively modulates the gut microbiota. Given the role of anthocyanins in the health benefits of berries, coupled with interactions of gut microbial metabolites with host health, the objective of this follow-up study was to further characterize the profile of functional metabolites in the gut microbiome modulated by anthocyanins. We utilized a berry-derived classic anthocyanin, cyanidin-3-glucoside (C3G), combined with a mouse model to probe C3G-associated functional metabolic products of gut bacteria through a mass spectrometry-based metabolomic profiling technique. Results showed that C3G substantially changed the gut microbiota of mice, including its composition and metabolic profile. A distinct metabolic profile in addition to a variety of key microbiota-related metabolites was observed in C3G-treated mice. Microbial metabolites involved in protein digestion and absorption were differently abundant between C3G-treated and control mice, which may be linked to the effects of berry consumption. Results of the present study suggest the involvement of the gut microbiota in the health benefits of C3G, providing evidence connecting the gut microbiota with berry consumption and its beneficial effects.

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“…The proteins expression levels of TRPA1, NPC1L1, PPARγ, SP-1, and SREBP-2 were examined by Western blot assay. Protein expression of cyclin dependent kinase 1 (Cyclin D1), one of the targeting proteins specific to cancer involved in cell cycle progression and proliferation [ 37 ], was also determined. Briefly, proteins were extracted from the cells in each group by RIPA lysis buffer (Beyotime, Shanghai, China), following which the concentration was determined using a BCA protein assay kit (Beyotime, Shanghai, China).…”

Section: Measurement Of Ca 2+ Signals/ca ...mentioning

confidence: 99%

Curcumin suppresses cell proliferation and reduces cholesterol absorption in Caco-2 cells by activating the TRPA1 channel

Qin

et al. 2023

Lipids Health Dis

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Background Curcumin (Cur) is a bioactive dietary polyphenol of turmeric with various biological activities against several cancers. Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths. Intestinal cholesterol homeostasis is associated with CRC. Chemotherapy for CRC is related to varied adverse effects. Therefore, natural products with anti-cancer properties represent a potential strategy for primary prevention of CRC. Methods The present study used Cur as a therapeutic approach against CRC using the Caco-2 cell line. The cells were treated with different concentrations of Cur for different duration of time and then the proliferation ability of cells was assessed using Cell Counting Kit-8 and 5-Ethynyl-2′-deoxyuridine assays. Oil red O staining and cholesterol assay kit were used to evaluate cellular lipid content and cholesterol outward transportation. Finally, the protein expressions of cholesterol transport-related protein and signal transduction molecules were assessed using Western blot assay. Results Cur inhibited cell proliferation in Caco-2 cells in a dose- and time-dependent manner by activating the transient receptor potential cation channel subfamily A member 1 (TRPA1) channel. Activation of the TRPA1 channel led to increased intracellular calcium, peroxisome proliferator-activated receptor gamma (PPARγ) upregulation, and the subsequent downregulation of the specificity protein-1 (SP-1)/sterol regulatory element-binding protein-2 (SREBP-2)/Niemann-Pick C1-like 1 (NPC1L1) signaling pathway-related proteins, and finally reduced cholesterol absorption in Caco-2 cells. Conclusions Cur inhibits cell proliferation and reduces cholesterol absorption in Caco-2 cells through the Ca2+/PPARγ/SP-1/SREBP-2/NPC1L1 signaling by activating the TRPA1 channel, suggesting that Cur can be used as a dietary supplement for the primary prevention of CRC. Graphical Abstract In Caco-2 cells, Cur first stimulates calcium influx by activating the TRPA1 channel, further upregulates PPARγ and downregulates SP-1/SREBP-2/NPC1L1 signaling pathway, and finally inhibits the absorption of cholesterol. TRPA1, transient receptor potential cation channel subfamily A member 1; NPC1L1, Niemann-Pick C1-like 1; PPARγ, peroxisome proliferator-activated receptor gamma; SP-1, specificity protein-1; SREBP-2, sterol regulatory element-binding protein-2; Cur, curcumin.

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Sirtuin1 (SIRT1) is involved in the anticancer effect of black raspberry anthocyanins in colorectal cancer

Cited by 10 publications

References 44 publications

Berries vs. Disease: Revenge of the Phytochemicals

Berries vs. Disease: Revenge of the Phytochemicals

Characterizing the Gut Microbial Metabolic Profile of Mice with the Administration of Berry-Derived Cyanidin-3-Glucoside

Curcumin suppresses cell proliferation and reduces cholesterol absorption in Caco-2 cells by activating the TRPA1 channel

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