Sistemas genéticos para un nuevo abordaje del riesgo de progresión de la retinopatía diabética

Pinazo-Durán, María Dolores; Zanón‐Moreno, Vicente; Lleó-Pérez, A.; García-Medina, J.J.; Galbis-Estrada, Carmen; Roig-Revert, M.J.; Marco-Ramírez, Carla; López-Gálvez, M.; Dolz-Marco, Rosa; Duarte, Lilianne; Borges, C. Campos; Salgado-Borges, José; Gallego-Pinazo, Roberto

doi:10.1016/j.oftal.2016.01.016

Cited by 8 publications

(2 citation statements)

References 42 publications

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“…Samples were assayed in duplicate. The expression values were calculated by the double delta Ct formula, as previously reported [34,35], and the results were expressed as fold changes in gene expression for each group and subgroup, at baseline.…”

Section: Biosample Processingmentioning

confidence: 99%

Clinical and Molecular-Genetic Insights into the Role of Oxidative Stress in Diabetic Retinopathy: Antioxidant Strategies and Future Avenues

et al. 2020

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Reactive oxygen species (ROS) overproduction and ROS-signaling pathways activation attack the eyes. We evaluated the oxidative stress (OS) and the effects of a daily, core nutritional supplement regimen containing antioxidants and omega 3 fatty acids (A/ω3) in type 2 diabetics (T2DM). A case-control study was carried out in 480 participants [287 T2DM patients with (+)/without (−) diabetic retinopathy (DR) and 193 healthy controls (CG)], randomly assigned to a daily pill of A/ω3. Periodic evaluation through 38 months allowed to outline patient characteristics, DR features, and classic/OS blood parameters. Statistics were performed by the SPSS 24.0 program. Diabetics displayed significantly higher circulating pro-oxidants (p = 0.001) and lower antioxidants (p = 0.0001) than the controls. Significantly higher plasma malondialdehyde/thiobarbituric acid reactive substances (MDA/TBARS; p = 0.006) and lower plasma total antioxidant capacity (TAC; p = 0.042) and vitamin C (0.020) was found in T2DM + DR versus T2DM-DR. The differential expression profile of solute carrier family 23 member 2 (SLC23A2) gene was seen in diabetics versus the CG (p = 0.001), and in T2DM + DR versus T2DM − DR (p < 0.05). The A/ω3 regime significantly reduced the pro-oxidants (p < 0.05) and augmented the antioxidants (p < 0.05). This follow-up study supports that a regular A/ω3 supplementation reduces the oxidative load and may serve as a dietary prophylaxis/adjunctive intervention for patients at risk of diabetic blindness.

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Section: Biosample Processingmentioning

confidence: 99%

Clinical and Molecular-Genetic Insights into the Role of Oxidative Stress in Diabetic Retinopathy: Antioxidant Strategies and Future Avenues

et al. 2020

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“…While most of the studies analysed the expression of miRNA in plasma or serum samples of T2D patients, several studies have investigated these epigenetic biomarkers in vitreous humour or tears [31,32]. In 2019, Chen and colleagues explored the miRNA and piwi-interacting RNA (piRNA) profile in the aqueous humour of nine PDR and nine cataract control patients [31].…”

Section: Mirna Profilingmentioning

confidence: 99%

Epigenetic Mechanisms in Type 2 Diabetes Retinopathy: A Systematic Review

Milluzzo

Maugeri

Barchitta

et al. 2021

IJMS

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Diabetic retinopathy (DR) is one of the main causes of vision loss in middle-aged economically active people. Modifiable (i.e., hyperglycaemia, hypertension, hyperlipidaemia, obesity, and cigarette smoke) and non-modifiable factors (i.e., duration of diabetes, puberty, pregnancy and genetic susceptibility) are involved in the development of DR. Epigenetic mechanisms, modulating the oxidative stress, inflammation, apoptosis, and aging, could influence the course of DR. Herein, we conducted a systematic review of observational studies investigating how epigenetics affects type 2 diabetes retinopathy (T2DR). A total of 23 epidemiological studies were included: 14 studies focused on miRNA, 4 studies on lnc-RNA, one study on both miRNA and lnc-RNA, and 4 studies on global or gene-specific DNA methylation. A direct relation between the dysregulation of miR-21, miR-93, and miR-221 and FPG, HbA1c, and HOMA-IR was identified. A panel of three miRNAs (hsa-let-7a-5p, hsa-miR-novel-chr5_15976, and hsa-miR-28-3p) demonstrated a good sensitivity and specificity for predicting T2DR. Little evidence is available regarding the possible role of the long non-coding MALAT1 dysregulation and MTHFR gene promoter hypermethylation. Despite these initial, encouraging findings potentially suggesting a role of epigenetics in T2DR, the use in clinical practice for the diagnosis and staging of this complication encounters several difficulties and further targeted investigations are still necessary.

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MicroRNA Profiling from Tears as a Potential Non-invasive Method for Early Detection of Diabetic Retinopathy

Wong,

Polkamp,

Farr

et al. 2023

Methods in Molecular Biology

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Sistemas genéticos para un nuevo abordaje del riesgo de progresión de la retinopatía diabética

Cited by 8 publications

References 42 publications

Clinical and Molecular-Genetic Insights into the Role of Oxidative Stress in Diabetic Retinopathy: Antioxidant Strategies and Future Avenues

Clinical and Molecular-Genetic Insights into the Role of Oxidative Stress in Diabetic Retinopathy: Antioxidant Strategies and Future Avenues

Epigenetic Mechanisms in Type 2 Diabetes Retinopathy: A Systematic Review

MicroRNA Profiling from Tears as a Potential Non-invasive Method for Early Detection of Diabetic Retinopathy

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