Sister chromatid exchange (SCE) analysis in periodontitis

Abstract

The data indicate that there the cyotogenetic damage in the aggressive type of periodontal disease is not greater than in chronic periodontitis and control subjects. Although no aberrant cytogenetic damage was observed in different forms of periodontitis compared to control, this does not discount the importance of other genetic factors in the pathogenesis of periodontal disease.

Assessment of Cytogenetic Damage to Exfoliated Gingival Cells in Patients with Chronic Generalized Periodontitis

“…Periodontal disease is an inflammatory process involving a set of changes that directly affect tissues that hold the teeth. There are few studies that assessed the occurrence of genetic damage associated with periodontal disease 6,[20][21][22][23][24][25][26] . Genotoxic damage of periodontal disease was followed by the micronucleus assay in peripheral lymphocytes 21,24, gingival 6,20 and buccal cells 22,26 , and by sister chromatid exchange method in peripheral blood lymphocytes 25 .…”

“…Emingil et al . used sister chromatid exchange analysis to evaluate cytogenetic damage in periodontitis patients, revealing no differences in tested parameters between periodontitis patients and healthy controls ( 25 ). However, Bloching et al .…”

Micronuclear and sister chromatid exchange analyses in peripheral lymphocytes of patients with oral lichen planus – a pilot study*

“…Aggressive and severe periodontal diseases are additional confounding factors that could affect SCE and MN frequencies (Emingil et al , 2002; Bloching et al , 2008). Oral clinical examination of subjects and control groups in our study revealed mild or moderate periodontal diseases and none had severe periodontitis.…”

“…Cytogenetic tests have often been applied to determine whether the presence of chromosome instability is related to the predisposition to a disease (Emingil et al , 2002). Among several available tests, sister chromatid exchange (SCE) and micronuclear (MN) analyses in peripheral blood lymphocytes are well‐established techniques aiming to evaluate the extent of cytogenetic damage (Latt and Schreck, 1980; Latt et al , 1980; Heddle et al , 1991; Kamboj and Mahajan, 2007).…”

Evaluation of micronuclear frequencies in both circulating lymphocytes and buccal epithelial cells of patients with oral lichen planus and oral lichenoid contact reactions

“…Cytogenetic tests have often been applied to determine whether the presence of chromosome instability is related to the predisposition to a disease (Emingil et al, 2002). Micronucleus (MN) assay in exfoliated cells is an effective technique to asses genotoxicity described for risk assessment of human populations exposed to carcinogens or mutagens (Tolbert et al, 1992;G omez-Arroyo et al, 2000).…”