Sister chromatid exchanges in human lymphocytes treated with silica.

Pairon, J.-C.; Jaurand, Marie-Claude; Kheuang, Laurence; Janson, X; Brochard, Patrick; Bignon, J.

doi:10.1136/oem.47.2.110

Cited by 14 publications

(15 citation statements)

References 22 publications

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“…However, silica has also recently been shown to generate free radicals detectable by electron spin resonance (ESR) spectroscopy under a variety of experimental conditions (16)(17)(18). It was proposed that these free radicals play an important role in the mechanism of silica toxicity and carcinogenicity (19)(20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

DNA Binding to Crystalline Silica Characterized by Fourier-Transform Infrared Spectroscopy

Mao¹,

Daniel²,

Whittaker³

et al. 1994

Environmental Health Perspectives

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The interaction of DNA with crystalline silica in buffered aqueous solutions at physiologic pH has been investigated by Fourier-transform infrared spectroscopy (FT-IR). In aqueous buffer, significant changes occur in the spectra of DNA and silica upon coincubation, suggesting that a DNA-silica complex forms as silica interacts with DNA. As compared to the spectrum of silica alone, the changes in the FT-IR spectrum of silica in the DNA-silica complex are consistent with an Si-O bond perturbation on the surface of the silica crystal. DNA remains in a B-form conformation in the DNA-silica complex. The most prominent changes in the DNA spectrum occur in the 1225 to 1000 cm1 region. Upon binding, the PO_ asymmetric stretch at 1225 cm1 is increased in intensity and slightly shifted to lower frequencies; the PO-symmetric stretch at 1086 cm1 is markedly increased in intensity; and the band at 1053 cm-, representing either the phosphodiester or the C-O stretch of DNA backbone, is significantly reduced in intensity.In D20 buffer, the DNA spectrum reveals a marked increase in intensity of the peak at 1086 cm-1 and a progressive decrease in intensity of the peak at 1053 cm-1 when DNA is exposed to increasing concentrations of silica. The carbonyl band at 1688 cm-diminishes and shifts to slightly lower frequencies with increasing concentrations of silica. The present study demonstrates that crystalline silica binds to the phosphate-sugar backbone of DNA. The close proximity of the silica surface to the DNA molecule, brought about by this binding, may contribute to DNA strand breakage produced by silica-derived free radicals. The ability of silica to form stable complexes with DNA may play an important role in the mechanisms of silica-induced toxicity and carcinogenesis. -Environ Health Perspect 102(Suppl 10):1 65-171 (1994)

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Section: Introductionmentioning

confidence: 99%

DNA Binding to Crystalline Silica Characterized by Fourier-Transform Infrared Spectroscopy

Mao¹,

Daniel²,

Whittaker³

et al. 1994

Environmental Health Perspectives

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“…Tridymite induced a significant number of sister chromatid exchanges (SCEs) in co-cultures of human lymphocytes and monocytes (P<0.05 compared with control cells) at doses of 5 and 50 µg/cm 2 (87.9% of the tridymite particles had a diameter <1 µm). However, the number of SCEs in purified human lymphocytes that were treated with the same doses of tridymite particles did not differ significantly from control cells [Pairon et al 1990]. Results of the same experiments with quartz did not yield a clear conclusion about the ability of quartz to induce a significant number of SCEs (Table 21) [Pairon et al 1990].…”

Section: Genotoxicity and Related Effectsmentioning

confidence: 88%

“…However, the number of SCEs in purified human lymphocytes that were treated with the same doses of tridymite particles did not differ significantly from control cells [Pairon et al 1990]. Results of the same experiments with quartz did not yield a clear conclusion about the ability of quartz to induce a significant number of SCEs (Table 21) [Pairon et al 1990]. …”

Section: Genotoxicity and Related Effectsmentioning

confidence: 88%

“…An in vivo treatment of rats with quartz induced mutation in rat alveolar epithelial cells (Table 21) [Driscoll 1995;. Pairon et al [1990] tested tridymite (i.e., Tridymite 118) and quartz (i.e., Min-U-Sil 5) particles for genotoxic effects. Tridymite induced a significant number of sister chromatid exchanges (SCEs) in co-cultures of human lymphocytes and monocytes (P<0.05 compared with control cells) at doses of 5 and 50 µg/cm 2 (87.9% of the tridymite particles had a diameter <1 µm).…”

Section: Genotoxicity and Related Effectsmentioning

confidence: 99%

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Summary of Notifiable Noninfectious Conditions and Disease Outbreaks: Surveillance for Silicosis — Michigan and New Jersey, 2003–2010

Filios

Mazurek²,

Schleiff³

et al. 2015

MMWR Morb. Mortal. Wkly. Rep.

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“…Kaolin is used in paper production, in paints, rubber, plastic, ceramic, chemical, pharmaceutical and cosmetic industries 1 . Pairon et al (15) in 1990 studied the interaction of Kaolin with cell lines and found a variety of membrane interactions and metabolic impairments. Kaolin in the recent past has been of interest in clinical studies due to its role in achieving haemostasis in Oculoplastic Surgery as a local application (16) or intra-abdominal surgery with Kaolin impregnated gauze as a leave-in substance for rapid hemorrhage control in critically injured patients in combat (17).…”

Section: Discussionmentioning

confidence: 99%

A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

et al. 2020

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Background: Adhesion formation after abdominal surgery is considered almost inevitable and a major cause of morbidity. Novel treatments have been proposed, however there is a lack of suitable small animal models for pre-clinical evaluation, mainly due to inconsistency in adhesion formation in positive control animals. Here, we propose a new rat model of abdominal adhesions using Kaolin as the adhesion-inducing agent at an optimized dosage for testing newer agents in respect to their anti-adhesive property.Materials and Methods: Twenty-five adult (8-10 week old) male Wistar albino rats underwent midline laparotomy and caecal abrasion and were randomized to receive topical applications of normal saline or different concentrations and volumes of a Kaolin-based formulation. At day 14 rats were humanely killed, and adhesions graded macroscopically by an investigator blinded to the treatment groups, using pre-determined adhesion scores and microscopically using histopathology.Results: Kaolin at 0.005 g/mL caused consistent adhesions without compromising rat viability. At higher doses significant morbidity and mortality was observed in the animals treated.Conclusions: Kaolin induced adhesion in a rat abdominal surgery model is reliable and can be safely used to test the efficacy of novel anti-adhesive formulations to prevent intra-abdominal adhesions.

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Sister chromatid exchanges in human lymphocytes treated with silica.

Cited by 14 publications

References 22 publications

DNA Binding to Crystalline Silica Characterized by Fourier-Transform Infrared Spectroscopy

DNA Binding to Crystalline Silica Characterized by Fourier-Transform Infrared Spectroscopy

Summary of Notifiable Noninfectious Conditions and Disease Outbreaks: Surveillance for Silicosis — Michigan and New Jersey, 2003–2010

A Novel Rat Model to Test Intra-Abdominal Anti-adhesive Therapy

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