Sisters doing it for themselves: extensive reproductive plasticity in workers of a primitively eusocial bee

Abstract

Plasticity is a key trait when an individual’s role in the social environment, and hence its optimum phenotype, fluctuates unpredictably. Plasticity is especially important in primitively eusocial insects where small colony sizes and little morphological caste differentiation mean that individuals may find themselves switching from non-reproductive to reproductive roles. To understand the scope of this plasticity, workers of the primitively eusocial sweat bee Lasioglossum malachurum were experimentally promoted to the reproductive role (worker-queens) and their performance compared with foundress-queens. We focussed on how their developmental trajectory as workers influenced three key traits: group productivity, monopolisation of reproduction, and social control of foraging nest-mates. No significant difference was found between the number of offspring produced by worker-queens and foundress-queens. Genotyping of larvae showed that worker-queens monopolised reproduction in their nests to the same extent as foundress queens. However, non-reproductives foraged less and produced a smaller total offspring biomass when the reproductive was a promoted worker: offspring of worker-queens were all males, which are the cheaper sex to produce. Greater investment in each offspring as the number of foragers increased suggests a limit to both worker-queen and foundress-queen offspring production when a greater quantity of pollen arrives at the nest. The data presented here suggest a remarkable level of plasticity and represent one of the first quantitative studies of worker reproductive plasticity in a non-model primitively eusocial species. Significance statement The ability of workers to take on a reproductive role and produce offspring is expected to relate strongly to the size of their colony. Workers in species with smaller colony sizes should have greater reproductive potential to insure against the death of the queen. We quantified the reproductive plasticity of workers in small colonies of sweat bees by removing the queen and allowing the workers to control the reproductive output of the nest. A single worker then took on the reproductive role and hence prevented her fellow workers from producing offspring of their own. These worker-queens produced as many offspring as control queens, demonstrating remarkable worker plasticity in a primitively eusocial species.

The evolution of morphological castes under decoupled control

“…Behavioural castes are often mutable, so that individuals can switch behavioural castes over their lifetimes. The inclusion of effects such as worker takeover, through which a worker can become a reproductive, may reduce the threshold worker productivity required for a daughter to favour joining a behavioural worker caste (Field et al, 1999; Toyoizumi and Field, 2014; Price and Field, 2022). Similarly, if a daughter can help raise more reproductive sisters (r=0.75) than reproductive brothers (r=0.25) by staying as a worker, it will further lower the productivity of a worker relative to a reproductive required for the daughter to favour joining a behavioural worker caste (Trivers and Hare, 1976).…”