Site, trigger, quenching mechanism and recovery of non-photochemical quenching in cyanobacteria: recent updates

Sonani, Ravi R.; Gardiner, Alastair T.; Rastogi, Rajesh P.; Cogdell, Richard J.; Robert, Bruno; Madamwar, Datta

doi:10.1007/s11120-018-0498-8

Cited by 15 publications

(5 citation statements)

References 67 publications

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“…In this study, non-photochemical quenching parameters was the most sensitive parameters to PA and GA, followed by photochemical quenching parameters or others [F v /F m , F' v /F' m , q TQ , and UQF (rel) ], and the least sensitive was cell densities. Under the stress of PA and GA, the PSII activity of M. aeruginosa was influenced at the beginning, but non-photochemical quenching could protect the photosynthesis (Sonani et al, 2018). The protective effects of non-photochemical quenching were gradually lost for further inhibition, and photochemical quenching parameters decreased followly, ultimatly, photosynthetic efficiency dropped significantly (Cummings et al, 2018).…”

Section: Discussionmentioning

confidence: 98%

Combined Effects of Allelopathic Polyphenols on Microcystis aeruginosa and Response of Different Chlorophyll Fluorescence Parameters

Huang

Zhu

et al. 2020

Front. Microbiol.

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Polyphenols are allelochemicals secreted by aquatic plants that effectively control cyanobacteria blooms. In this study, sensitive response parameters (including CFPs) of Microcystis aeruginosa were explored under the stress of different polyphenols individually and their combination. The combined effects on M. aeruginosa were investigated based on the most sensitive parameter and cell densities. For pyrogallic acid (PA) and gallic acid (GA), the sensitivity order of parameters based on the EC50 values (from 0.73 to 3.40 mg L–1 for PA and from 1.05 to 2.68 mg L–1 for GA) and the results of the hierarchical cluster analysis showed that non-photochemical quenching parameters [NPQ, qN, qN(rel) and qCN] > photochemical quenching parameters [YII, qP, qP(rel) and qL] or others [Fv/Fm, F’v/F’m, qTQ and UQF(rel)] > cell densities. CFPs were not sensitive to ellagic acid (EA) and (+)-catechin (CA). The sensitivity order of parameters for M. aeruginosa with PA-GA mixture was similar to that under PA and GA stress. The quantitative (Toxicity Index, TI) and qualitative (Isobologram representation) methods were employed to evaluate the combined effects of PA, GA, and CA on M. aeruginosa based on cell densities and NPQ. TI values based on the EC50 cells suggested the additive effects of binary and multiple polyphenols, but synergistic and additive effects according to the EC50 NPQ (varied from 0.16 to 1.94). In terms of NPQ of M. aeruginosa, the binary polyphenols exhibited synergistic effects when the proportion of high toxic polyphenols PA or GA was lower than 40%, and the three polyphenols showed a synergistic effect only at the ratio of 1:1:1. Similar results were also found by isobologram representation. The results showed that increasing the ratio of high toxic polyphenols would not enhance the allelopathic effects, and the property, proportion and concentrations of polyphenols played an important role in the combined effects. Compared with cell densities, NPQ was a more suitable parameter as evaluating indicators in the combined effects of polyphenols on M. aeruginosa. These results could provide a method to screen the allelochemicals of polyphenols inhibiting cyanobacteria and improve the inhibitory effects by different polyphenols combined modes.

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Section: Discussionmentioning

confidence: 98%

Combined Effects of Allelopathic Polyphenols on Microcystis aeruginosa and Response of Different Chlorophyll Fluorescence Parameters

Huang

Zhu

et al. 2020

Front. Microbiol.

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“…Excitation energy is funneled along the rods to the core which finally can attach to either photosystem I or II. NPQ occurs at the core level and involves a water-soluble protein called orange carotenoid protein (OCP). − …”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Activation in the Orange Carotenoid Protein Revealed by Molecular Dynamics

et al. 2020

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Light-harvesting in photosynthesis is accompanied by photoprotective processes. In cyanobacteria, the photoprotective role is played by a specialized complex, the orange carotenoid protein, which is activated by strong blue-green light. This photoactivation involves a unique series of structural changes which terminate with an opening of the complex into two separate domains, one of which acts as a quencher for the lightharvesting complexes. Many experimental studies have tried to reveal the molecular mechanisms through which the energy absorbed by the carotenoid finally leads to the large conformational change of the complex. Here, for the first time, these mechanisms are revealed by simulating at the atomistic level the whole dynamics of the complex through an effective combination of enhanced sampling techniques. On the basis of our findings, we can conclude that the carotenoid does not act as a spring that, releasing its internal strain, induces the dissociation, as was previously proposed, but as a "latch" locking together the two domains. The photochemically triggered displacement of the carotenoid breaks this balance, allowing the complex to dissociate.

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“…Selective interaction with OCP lacking the NTE, i.e., the ∆NTE mutant, (submicromolar K d ) 30 , and with individual CTD, but not individual NTD 25 , 33 , implied that the crucial FRP-binding site is located on the CTD, although the possibility of secondary site(s) was also proposed 24 , 30 , 34 . Many observations suggested FRP monomerization upon its interaction with various OCP forms 24 , 25 , 30 , 32 , however, the necessity and role of this process was unclear 35 , 36 . Intriguingly, low-homology FRP from Anabaena variabilis and Arthrospira maxima demonstrated the ability to perform on OCP from Synechocystis sp .…”

Section: Introductionmentioning

confidence: 99%

OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria

et al. 2018

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In cyanobacteria, high light photoactivates the orange carotenoid protein (OCP) that binds to antennae complexes, dissipating energy and preventing the destruction of the photosynthetic apparatus. At low light, OCP is efficiently deactivated by a poorly understood action of the dimeric fluorescence recovery protein (FRP). Here, we engineer FRP variants with defined oligomeric states and scrutinize their functional interaction with OCP. Complemented by disulfide trapping and chemical crosslinking, structural analysis in solution reveals the topology of metastable complexes of OCP and the FRP scaffold with different stoichiometries. Unable to tightly bind monomeric FRP, photoactivated OCP recruits dimeric FRP, which subsequently monomerizes giving 1:1 complexes. This could be facilitated by a transient OCP–2FRP–OCP complex formed via the two FRP head domains, significantly improving FRP efficiency at elevated OCP levels. By identifying key molecular interfaces, our findings may inspire the design of optically triggered systems transducing light signals into protein–protein interactions.

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Site, trigger, quenching mechanism and recovery of non-photochemical quenching in cyanobacteria: recent updates

Cited by 15 publications

References 67 publications

Combined Effects of Allelopathic Polyphenols on Microcystis aeruginosa and Response of Different Chlorophyll Fluorescence Parameters

Combined Effects of Allelopathic Polyphenols on Microcystis aeruginosa and Response of Different Chlorophyll Fluorescence Parameters

Molecular Mechanisms of Activation in the Orange Carotenoid Protein Revealed by Molecular Dynamics

OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria

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