Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleases

Scott, Walter A.; Wigmore, Dianne J.

doi:10.1016/0092-8674(78)90073-9

Cited by 231 publications

(65 citation statements)

References 27 publications

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“…Such regions are generally hypersensitive to DNase I (Cremisi, 1981;Groudine et al, 1981;Herbormel et al, 1981;Jakobovits, 1980;McGhee et al, 1981;Samal et al, 1981;Saragosti et al, 1980;Scott and Wigmore, 1978;Stalder et al, 1980;Varshavsky et al, 1979;Weintraub et al, 1981; Chromatin alteration in actively transcribed ovalbumin gene Wong et al, 1981;Wu and Gilbert, 1981) and in some cases they are more accessible to micrococcal nuclease Varshavsky et al, 1978) and restriction endonucleases (Kornberg, 1977;Sundin and Varshavsky, 1979;Varshavsky et al, 1979). In addition, electron microscopic studies have revealed the specific absence of nucleosomes from a region of several hundred base pairs upstream from the transcription units of both viral (Herbormel et al, 1981;Jakobovits et al, 1980;Saragosti et al, 1980) and cellular (Lamb and Daneholt, 1979) (LeMeur et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, a region of -1500 bp, which precedes the first exon (L), is even more rapidly digested by DNase I than the gene itself (M. Bellard, unpublished results). This hypersensitivity does not generate the preferential cuts which have been detected at the 5'-end of many active genes (Cremisi, 1981;Groudine et al, 1981;Herbormel et al, 1981;Jakobovits et al, 1980;McGhee et al, 1981;Samal et al, 1981;Saragosti et al, 1980;Scott and Wigmore, 1978;Stalder et al, 1980;Varshavsky et al, 1979;Weintraub et al, 1981;Wong et al, 1981;Wu, 1980;Wu and Gilbert, 1981), possibly because it is too extensive and homogeneous.…”

Section: Discussionmentioning

confidence: 99%

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Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Bellard¹,

Dretzen²,

Bellard³

et al. 1982

The EMBO Journal

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We examined the chromatin organization of -3 kb of DNA in the 5' -end flanking region of the ovalbumin gene in chicken erythrocyte and oviduct cell nuclei. With specific DNA probes and an indirect end-labeling technique, we analysed the pattern of the DNA fragments obtained after micrococcal nuclease digestion and generated comparative maps of the nuclease cuts. This region of the chicken genome displays a "typical" chromatin arrangement in erythrocyte nuclei, with nucleosomes apparently positioned at random. In contrast, in oviduct nuclei, the same region has an "altered" chromatin structure, and lacks a typical nucleosomal array. The existence of specifically positioned proteins and of alterations in the DNA secondary structure in this region of the oviduct chromatin is suggested by comparison of the nuclease cleavage maps which reveals specific changes: disappearance of nuclease cuts present in "naked" and erythrocyte chromatin DNAs, and appearance of new cuts absent from these DNAs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Bellard¹,

Dretzen²,

Bellard³

et al. 1982

The EMBO Journal

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“…SV40 DNA Forms Conversion Assay-Naked SV40 DNA or SV40 minichromosomes (150 ng, isolated as described elsewhere (20,21)) was incubated at 37°C with 1-CBI in 20 l of TE buffer for up to 16 h (naked * This study was supported in part by National Institutes of Health Grants CA80939 and CA16056 (to T. A. B.)…”

Section: Methodsmentioning

confidence: 99%

Cell-free and Cellular Activities of a DNA Sequence Selective Hairpin Polyamide-CBI Conjugate

Wang

Dzięgielewski

Chang

et al. 2002

Journal of Biological Chemistry

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Alkylating agents are generally highly reactive with DNA but demonstrate limited DNA sequence selectivity. In contrast, synthetic pyrrole-imidazole polyamides recognize specific DNA sequences with high affinity but are unable to permanently damage DNA. An eight-ring hairpin polyamide conjugated to the alkylating moiety cyclopropylpyrroloindole, related to the natural product CC-1065, affords a conjugate 1-CBI (polyamide 1-CBI (1-(chloromethyl)-5-hydroxyl-1,2-dihydro-3H-benz[e]indole) conjugate), which binds to specific sequences in the minor groove of DNA and alkylates a single adenine flanking the polyamide binding site. In this study, we show that 1-CBI alkylates DNA in both plasmid and intracellular minichromosomal form and inhibits DNA replication under both cell-free and cellular conditions. In addition, it inhibits cell growth and arrests cells in the G 2 /M phase of the cell cycle.

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“…SV40 has been employed as a model system for these studies because the circular genome of this DNA tumor virus is well characterized, and the locations of the five viral genes, origin of replication, and viral regulatory sequences are known (19,20). The structure of viral chromatin has been probed with a variety of nucleases, revealing a DNase I hypersensitive and nucleosome-free region adjacent to the origin of replication and encompassing the viral regulatory sequences (21)(22)(23). We have found that BLM cuts purified SV40 DNA with strong preference for several specific sites.…”

Section: Introductionmentioning

confidence: 99%

Site specificity of bleomycin cleavage in purified and intracellular simian virus 40 DNA

1987

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The sites of bleomycin-induced cleavage of purified and intracellular simian virus 40 DNA were examined. Breaks in purified DNA were mapped to several discrete sites that were distributed throughout the viral genome, but were not associated with a conmnon genetic element. Double-stranded breaks were made in positions of the first single-stranded nick, and regions of cuts were unaffected by changes In DNA superhelicity. Bleomycin cut intracellular chromosomes at the same sites that were cleaved in purified DNA. These results indicate that SV40 DNA contains DNA secondary structures that are highly preferred sites for BLM cleavage. These conformations appear to be unaffected by nucleoproteins bound to DNA.

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Sites in simian virus 40 chromatin which are preferentially cleaved by endonucleases

Cited by 231 publications

References 27 publications

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Cell-free and Cellular Activities of a DNA Sequence Selective Hairpin Polyamide-CBI Conjugate

Site specificity of bleomycin cleavage in purified and intracellular simian virus 40 DNA

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