Sites of strand breakage in DNA irradiated by fast neutrons

Isabelle, V.; Franchet-Beuzit, J.; Sabattier, R.; Spotheim-Maurizot, M.; Charlier, Michel

doi:10.1016/0300-9084(94)90012-4

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…Other methods of hydroxyl radical generation have been developed; these include the following: production of HO • radicals by photosensitization − and photolysis of N -hydroxypyridine-2(1H)-thione; ,, X-ray radiolysis using a rotating anode source; γ-ray radiolysis; − electron radiolysis; ,, use of fast neutrons; − and sonolysis of water. − …”

Section: 9 Other Hydroxyl Radical Generation Techniquesmentioning

confidence: 99%

Hydroxyl Radical-Mediated Modification of Proteins as Probes for Structural Proteomics

2007

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Section: 9 Other Hydroxyl Radical Generation Techniquesmentioning

confidence: 99%

Hydroxyl Radical-Mediated Modification of Proteins as Probes for Structural Proteomics

2007

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“…For many years, studies of the inducement of radiation on DSB have been dominated by the random breakage theory [9,10] , which argues that DNA segments are distributed randomly after irradiation. However, in this study, the dividing line around 5 kbp of the DNA releasing segments after radiation was obvious in the electrophoresis image.…”

Section: Niger Sporesmentioning

confidence: 99%

Fast Neutron Radiation Effects on Bacillus Subtili

Chen

Ren

Zhang

et al. 2009

Plasma Sci. Technol.

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To examine the sterilizing effect and mechanism of neutron radiation, Bacillus subtilis var. niger. strain (ATCC 9372) spores were irradiated with the fast neutron from the Chinese fast burst reactor II(CFBR-II). The plate-count results indicated that the D10 value was 384.6 Gy with a neutron radiation dose rate of 7.4 Gy/min. The rudimental catalase activity of the spores declined obviously with the increase in the radiation dose. Meanwhile, under the scanning electron microscope, no visible influence of the neutron radiation on the spore configuration was detected even if the dose was increased to 4 kGy. The content and distribution of DNA double-strand breaks induced by neutron radiation at different doses were measured and quantified by pulsedfield gel electrophoresis (PFGE). Further analysis of the DNA release percentage (P R), the DNA breakage level (L), and the average molecular weight, indicated that DNA fragments were obviously distributed around the 5 kb regions at different radiation doses, which suggests that some points in the DNA molecule were sensitive to neutron radiation. Both P R and L varied regularly to some extent with the increase in radiation dose. Thus neutron radiation has a high sterilization power, and can induce falling enzyme activity and DNA breakage in Bacillus subtilis spores

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A Survey of the Sequence-Specific Interaction of Damaging Agents with DNA: Emphasis on Antitumor Agents

Murray

1999

Progress in Nucleic Acid Research and Molecular Biology

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Sites of strand breakage in DNA irradiated by fast neutrons

Cited by 6 publications

References 24 publications

Hydroxyl Radical-Mediated Modification of Proteins as Probes for Structural Proteomics

Hydroxyl Radical-Mediated Modification of Proteins as Probes for Structural Proteomics

Fast Neutron Radiation Effects on Bacillus Subtili

A Survey of the Sequence-Specific Interaction of Damaging Agents with DNA: Emphasis on Antitumor Agents

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