Sitotoksik Analizlerin Su Kalitesi Değerlendirmeleri Üzerine Etkinliğinin Belirlenmesi

Abstract

In the studies carried out by us, IC50 value of water was determined by using mesenchymal stem cells and endothelial cell lines in in vitro cultures and positive and negative effect doses on the cells were determined. Depending on the results obtained, the effects on the whole organism, if necessary, can be examined in vivo on subjects. For this purpose, young wistar rats are divided into experimental groups (experimental and control) and their water needs are met with water samples obtained from the study area for 3 to 6 months. Blood and urine samples were taken from the subjects in each group at certain time points and the changes were recorded by analyzing the routine biochemistry and hemogram. In addition, the subjects in the sampling are sacrificed at monthly intervals and general examination of endogenous tissues are performed and liver, kidney and heart tissue samples are taken for histological and chemical analyzes. Some of the tissue samples are homogenized using a microwave and changes in elemental content are determined by ICP_OES. The other part is fixed in 10% formaldehyde and then 5 µm thin sections are examined histopathologically according to freeze sectioning method. These studies showed that although the known methods used in water analysis are very valuable, the water quality study carried out by cytotoxic analysis method provides reliability in terms of observing the direct effects of water quality on living tissue. In the present study, in vitro cytotoxicity studies for water samples taken from İncesu Basin are given as examples because of their striking results. Based on the obtained results, it is suggested that in vitro cytotoxicity tests should be added to routine water quality analyzes.

Effect of Drought and UV-B Stress on Leaf Morphology of Ash-Leaved Maple and Sycamore Maple

“…Global climate change (Cantürk and Kulaç, 2021;Koç, 2022b), urbanization (Dogan et al, 2022;Zeren Cetin et al, 2023;Cobanoglu et al, 2023), and environmental pollution (Mutlu and Aydın Uncumusaoğlu, 2018;Emin et al, 2020;Demir et al, 2021;Tokatli et al, 2021;Çobanoğlu et al, 2022) are interconnected global problems worldwide, which have entered a very rapid process of change with the effect of the industrial revolution. It is stated that these problems are directly and indirectly related to each other Sulhan et al, 2022;Kuzmina et al, 2023) and can affect both the life of all living things in the world and ecosystems in a devastating level (Varol et al, 2021;Koç, 2022c).…”