A retrospective study was conducted on the prevalence of rabies at selected districts of Tigray (Gantafeshum, Enderta, Mekelle, and Adigrat) from 2008 to April 2009 for human and animals. In addition to the retrospective study, a questionnaire was also prepared and circulated to 420 study participants. As per the information collected, 267 animals and humans died of rabies and the highest percentage of cases was in dogs (70.8%) followed by bovine (9.80%), human (7.10%), equine (6.75%), hyena (3.75%) and ovine (1.87%). On comparing the rabies cases in the areas, it was noticed that maximum cases occurred in Gantafeshm (32. 5%). The study revealed that rabies was prevalent in the districts. Rabies cases in human being were observed to be more in people living in villages than those living in urban areas. Thus, awareness creation about the disease should be recommended in order to design effective prevention and control methods.