Six antigenic groups within the genus pestivirus as identified by cross neutralization assays

Dekker, A.; Wensvoort, G.; Terpstra, C.

doi:10.1016/0378-1135(95)00116-6

Cited by 60 publications

(31 citation statements)

References 19 publications

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“…Correlation between genetic subgrouping of BVDV1 and antigenic differences might exist , yet it has not been easily demonstrated by Mab-binding and/or cross-neutralization (Dekker et al, 1995;Paton et al, 1995;Vilcek et al, 2001). Rather, genetic analysis of BVDV1 isolates from Europe and North America suggests that segregation of BVDV1 into subgenotypes seems to be more correlated with geographical location (Van Rijn et al, 1997;Vilcek et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: evidence for a subgenotype within BVDV-2

et al. 2002

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Flores, Eduardo F.; Ridpath, Julia F.; Weiblen, Rudi; Vogel, Fernanda S. F.; and Gil, Laura H.V.G., "Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: evidence for a subgenotype within BVDV-2" (2002 AbstractPhylogenetic analysis divides bovine viral diarrhea viruses (BVDV) into two different genotypes (BVDV1 and BVDV2). BVDV1 strains have been further subdivided into two to 11 subgenotypes. Phylogenetic analysis of BVDV2 isolates, however, has not been able to identify discrete subgenotypes. In this study, we identified six South American BVDV2 strains and one North American BVDV2 strain that cluster to a separate genetic group within BVDV2, thus representing a distinct subgenotype. The 5% untranslated region (UTR) sequence homology between these six strains and other BVDV2 from North America, Europe and Asia (81.7%) is lower than the homology used to segregate BVDV1 into BVDV1a and BVDV1b (83.6%). Most nucleotide differences observed between the two subgroups of BVDV2 were concentrated in two regions, which also harbor most of the differences seen between BVDV1a and BVDV1b. To determine if this segregation was real, an additional analysis was performed comparing NS2/3 sequences. Analysis of a conserved sequence located between nucleotides 6670 and 7186 of the NS2/3 coding region also segregated these isolates to a separate group. The sequence homology between the two subgroups (86.3%) was higher than the homology in the 5%UTR (81.7%), with mean sequence homologies of 91 and 87.2% within the proposed subgroups. In contrast to the 5%UTR, alignment of the NS2/3 sequences revealed nucleotide differences distributed across the region. These results demonstrate that BVDV2 isolates cluster to two genetically distinct subgroups within BVDV2. The differences in both the 5%UTR and NS2/3 are consistent and justify this segregation. We suggest that BVDV2 may thereafter be subgenotyped into BVDV2a and BVDV2b. The existence of subgroups within the BVDV2 genotype with genetic heterogeneity similar to that seen among BVDV1 subgroups argues against BVDV2 isolates arising from BVDV1 in a recent evolutionary event. Unless the evolutionary clocks for BVDV1 and BVDV2 isolates tick along at different rates, these results indicate that BVDV2 have existed as long as BVDV1.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: evidence for a subgenotype within BVDV-2

et al. 2002

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“…In the latter species, titres of neutralizing BVDV, BVD, CSF antibodies have been found to decrease slowly, usually over a period of several years (15,20,23,25,26) The neutralization test has. in general, a high specificity, which has also been found to be the case when testing for antibodies against BVDV, BVD virus in sheep and cattle (3,4,5,6,7,9,15,19,20). The level of antibodies which had produced had a high titres during pregnancy must have been infected with pestivirus during this period, and probably.…”

Section: Resultsmentioning

confidence: 76%

“…Similar variations have been from other countries in some goats and sheep (18,19,20). By serological examination (1,6,7,8,9,10,11,15) found ovine field strains of BD virus to be antigenically more closely related to the Oregon Strain than to the NADL strain. In the present study, investigations sera in cattle, heifers, sheep, ewes showed a lower antibody titre against the Alfort-187 strain than against the other strains.…”

Section: Resultsmentioning

confidence: 99%

“…Cattle, heifers, sheep, ewes, sows and swine with gestation failure were sampled again after abortion. The sera were stored at -20 °C, until tested in two-fold dilutions for neutralizing antibodies against the cytopathic OREGON C24, NADL, OSLOSS and Alfort-187 strains of BVDV and CSF virus, as described by (7,8,9,10,11). Lowest serum dilution was 1 in 8.…”

Section: Virological Examinationsmentioning

confidence: 99%

“…Some investigations found BVD Strains that reacted more strongly with certain BVDV antisera than with BVD antiserum (8,9,10,12).…”

Section: Introductionmentioning

confidence: 99%

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Six antigenic groups within the genus pestivirus as identified by cross neutralization assays

Cited by 60 publications

References 19 publications

Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: evidence for a subgenotype within BVDV-2

Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: evidence for a subgenotype within BVDV-2

Pestivirus Infections in Bulgaria. Epidemiological Studies in Cattle, Sheep, Swine and Goats

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