Six-month results of suprachoroidal adipose tissue-derived mesenchymal stem cell implantation in patients with optic atrophy: a phase 1/2 study

Öner, Ayşe; Gönen, Zeynep Burçin; Sevim, Duygu Gülmez; Kahraman, Neslihan Sinim; Ünlü, Metin

doi:10.1007/s10792-019-01141-5

Cited by 20 publications

(23 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, the activated transcription factors enter the nucleus and bind directly or indirectly to DNA to regulate the cell transition from G0 to G1 necessary to enter the cellular growth cycle and gene expression, promoting an increased synthesis of proteins, including enzymes and cytokines. These final products play a key role in cell survival, including mitosis, migration, and cellular differentiation (Limoli et al, 2014;Oner et al, 2019;Limoli et al, 2016;Limoli et al, 2017;Limoli, 2014 b;Finklestein and Plmaritoglou, 2001;Garcia et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an autograft of mesenchimal cells of fat cells and PRP was performed using LRRT (Limoli et al, 2014;Oner et al, 2019;Limoli et al, 2016;Limoli et al, 2017) (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

“…It consists in a graft of the before mentioned mesenchymal cell types, which are adypose cells, adipose derived stem cells (ADSCs) contained in the stromal vascular fraction (SVF) of adipose tissue and plateletes (PLT) recovered in the platelet-rich plasma (PRP) (Gentile et al, 2012). Each eye received a graft of orbital fat between the choroid and sclera in a scleral pocket created with deep sclerectomy to expose the surface of the choroidal space (Limoli et al, 2014;Oner et al, 2019;Limoli et al, 2016;Limoli et al, 2017). In particular, we estimated the VP, i.e., BCVA, close-up visus in print size, sensitivity in dB measured by MY, and visus obtained after rehabilitation through magnifying visual devices in both groups at baseline (T0) and at 6 months (T180).…”

Section: Methodsmentioning

confidence: 99%

“…Since the GFs secreted by MSCs in the retinal environment can be exploited to provide a therapeutic action, we consider the possibility of treating the retina affected by RP with a surgical technique called Limoli Retinal Restoration Technique (LRRT), described in previous works (Fig. 1) (Gentile et al, 2012;Limoli et al 2014;Oner et al, 2019;Limoli et al 2016;Limoli et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mesenchymal stem cell surgery, rescue and regeneration in retinitis pigmentosa: clinical and rehabilitative prognostic aspects

Limoli¹,

Limoli²,

Morales

2020

Restorative Neurology and Neuroscience

View full text Add to dashboard Cite

Purpose: To assess whether treatment with the Limoli Retinal Restoration Technique (LRRT) can be performed in patients with retinitis pigmentosa (RP), grafting the autologous cells in a deep scleral pocket above the choroid of each eye to exert their beneficial effect on the residual retinal cells. Methods: The patients were subjected to a complete ophthalmological examination, including best corrected visual acuity (BCVA), close-up visus measurements, spectral domain-optical coherence tomography (SD-OCT), microperimetry (MY), and electroretinography (ERG). Furthermore, the complete ophthalmological examination was carried out at baseline (T0) and at 6 months (T180) after surgery. The Shapiro-Wilk test was used to assess the normality of distribution of the investigated parameters. A mixed linear regression model was used to analyse the difference in all the studied parameters at T0 and T180, and to compare the mean change between the two groups. All statistical analyses were performed with STATA 14.0 (Collage Station, Texas, USA). Results: LRRT treatment was performed in 34 eyes of 25 RP patients recruited for the study. The eyes were classified in two groups on the basis of foveal thickness (FT) assessed by SD-OCT: 14 eyes in Group A (FT ≤ 190 m) and the remaining 20 ones in Group B (FT > 190 m). Although it had not reached the statistical significance, Group B showed a better improvement in BCVA, residual close-up visus and sensitivity than Group A. Conclusions: Previous studies have described the role of LRRT in slowing down retinal degenerative diseases. Consequently, this surgical procedure could improve the clinical and rehabilitative prognostic parameters in RP patients. On the other hand, further clinical research and studies with longer follow-up will be needed to evaluate its efficacy.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Therefore, an autograft of mesenchimal cells of fat cells and PRP was performed using LRRT (Limoli et al, 2014;Oner et al, 2019;Limoli et al, 2016;Limoli et al, 2017) (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mesenchymal stem cell surgery, rescue and regeneration in retinitis pigmentosa: clinical and rehabilitative prognostic aspects

Limoli¹,

Limoli²,

Morales

2020

Restorative Neurology and Neuroscience

View full text Add to dashboard Cite

show abstract

“…In our experience, in order to have the therapeutic action of growth factors in the retinal environment, we have explored the possibility of treating the dystrophic retina with the implantation of the cell types of mesenchymal origin mentioned above, in detail adipose stromal cells (ASC), stem cells derived from adipose tissue (ADSC) contained in the stromal-vascular fraction (SVF) of adipose tissue, and platelets (PLT) recovered in platelet-rich plasma (PRP) [47][48][49]. To this end, we used a surgical technique called Limoli retinal restoration technique (LRRT), described in previous works (Figure 2) [27, 50,51]. The autotransplantation of ADSC, ACS, and PLT above the choroid plane improves the incretion of the bioactive factors produced in the choroidal flow and, consequently, promotes their widespread diffusion through the retinal tissue, finally exuding in the vitreous body.…”

Section: Cell Therapy and Routes Of Administrationmentioning

confidence: 99%

Regenerative Medicine and Eye Diseases

Contento¹,

Florido²,

F³

et al. 2020

Regenerative Medicine

View full text Add to dashboard Cite

The chapter examines the use of stem cells in ophthalmological pathologies affecting both the anterior and posterior segments. The authors review the clinical trials that have most contributed to defining the role and potential of stem cell regenerative therapy in corneal and retinal pathology. The results described in the scientific literature are analyzed and commented, without neglecting the possible side effects related to the use of this therapy. Within the anterior segment, the greatest efforts were made to study the possible uses of limbal epithelial stem cells (LESCs). They were the first stem cells to be discovered at the level of the anterior segment and currently the only ones involved in clinical practice with satisfactory results. At this juncture there have been significant successes in the treatment of corneal stem cell deficiency and of corneal scars. The chapter later investigates the possible applications of stem cell therapy in degenerative retinal diseases, with particular reference to retinitis pigmentosa, Stargardt's disease, and age-related macular degeneration. It then describes how the use of cell therapies, in particular those that use ADSC, can contribute, through various methods, to the containment of the evolution of retinal degenerative diseases. These mechanisms cover various biological aspects and can be summarized as follows: neurotrophism, oxidation, vascular changes, apoptosis, inflammation, or immunology. The ophthalmological modalities of the cell graft and what is the ideal approach for an ophthalmological cellular surgery are later on described. Finally, the technique used by the author and the possible outcomes in the course of degenerative retinopathy are described.

show abstract

Inherited eye diseases in Turkey: Current approaches and future directions

Tuncay

Ergün

Öner

et al. 2020

American J of Med Genetics Pt C

Self Cite

View full text Add to dashboard Cite

The aim of this review is to reveal Turkey's current status of medical practice in inherited eye diseases and the necessary steps to improve healthcare services and research activities in this area. Since consanguinity rate is high, disease burden is estimated to be high in Turkey. Universal health insurance system, easily accessible medical specialists, increasing genetic test, and counseling opportunities are the key advantages of Turkey's healthcare system. However, specialized clinics for inherited eye diseases, low-vision rehabilitation services, training of ophthalmologists about the recent developments in ocular genetics, and multidisciplinary translational research are the main headlines needed to be focused for better health services and successful research in Turkey.

show abstract

Six-month results of suprachoroidal adipose tissue-derived mesenchymal stem cell implantation in patients with optic atrophy: a phase 1/2 study

Cited by 20 publications

References 16 publications

Mesenchymal stem cell surgery, rescue and regeneration in retinitis pigmentosa: clinical and rehabilitative prognostic aspects

Mesenchymal stem cell surgery, rescue and regeneration in retinitis pigmentosa: clinical and rehabilitative prognostic aspects

Regenerative Medicine and Eye Diseases

Inherited eye diseases in Turkey: Current approaches and future directions

Contact Info

Product

Resources

About