Six New Phenolic Glycosides and a New Ceramide from the Flowers of Wedelia biflora and Their Cytotoxicity Against Some Cancer Cell Lines

Abstract

The plant Wedelia biflora has been used in traditional medicine in India and Vietnam to treat various symptoms. However, the chemical composition of its flowers remains mostly unknown. Therefore, we now report the isolation and structural elucidation of six new phenolic glycosides {wedebicoside A-F (1-6)} and one new ceramide [wedebiceramide (9)], together with six known compounds, 1-O-(2',4'-diangeloyloxy-β-D-fucopyranosyl)-6-hydroxythymol (7), 1-O-[2',4'-diangeloyloxy-3'-(3''-angeloyloxy-β-D-fucopyranosyl)-β-D-fucopyranosyl]-6-hydroxythymol (8), anhydrosecoisolariciresinol (10), friedeline (11), epifriedelanol (12) and stigmasterol (13) from the flowers of Wedelia biflora. Their structures were established by 1D and 2D NMR spectroscopy, as well as by high resolution ESI-MS analysis and comparison with literature data. The cytotoxic activities against HeLa, MCF-7 and NCI-H460 were evaluated on some purified compounds at the concentration of 100 μg/mL. Compounds 1, 2, 3 and 5 showed strong cytotoxic activities against three surveyed cancer cell lines. Consequently, this study elucidated the phytochemical composition of W. biflora, as well as the potential use of some of the new compounds against some cancers.

Integration of Traditional and Western Medicine in Vietnamese Populations: A Review of Health Perceptions and Therapies

“…Specifically, C. latifolium extracts and its alkaloid fraction inhibit proliferation of lymphoma cells [40] and contribute to the recovery of the immune function [43]. The plant Wedelia biflora has also been used in Indian and Vietnamese TM to treat various conditions, and data suggest that some compounds isolated from W. biflora are effective against some cancers [41]. Another traditional Vietnamese herb, Zanthoxylum avicennae, inhibits proliferation of HA22T human hepatocellular carcinoma cells via PP2A activation [42].…”