Six-year retrospective surveillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada

Jf, Lew; Ri, Glass; Petric, Martin; Cw, LeBaron; Gw, Hammond; Se, Miller; Robinson, Christine C.; Boutilier, Jordan; Riepenhoff-Talty, M; Cm, Payne

doi:10.1097/00006454-199010000-00005

Cited by 63 publications

(25 citation statements)

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“…In fact, all the targeted viruses were found to be more prevalent in young children than in adults (Table 4). Like norovirus GI/GII (45,46), both astrovirus (47,48) and sapovirus (49) are thought to be more prevalent during winter months. Interestingly, both viruses were detected during this study during the spring and summer months.…”

Section: Discussionmentioning

confidence: 99%

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

et al. 2015

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fThe appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. Clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. Additionally, there are limited or no testing methods routinely available for most diarrheagenic Escherichia coli strains, astroviruses, and sapoviruses. This study assessed the performance of the FilmArray Gastrointestinal (GI) Panel for the simultaneous detection of 22 different enteric pathogens directly from stool specimens: Campylobacter spp., Clostridium difficile (toxin A/B), Plesiomonas shigelloides, Salmonella spp., Vibrio spp., Vibrio cholerae, Yersinia enterocolitica, enteroaggregative E. coli, enteropathogenic E. coli, enterotoxigenic E. coli, Shiga-like toxin-producing E. coli (stx 1 and stx 2 ) (including specific detection of E. coli O157), Shigella spp./enteroinvasive E. coli, Cryptosporidium spp., Cyclospora cayetanensis, Entamoeba histolytica, Giardia lamblia, adenovirus F 40/41, astrovirus, norovirus GI/GII, rotavirus A, and sapovirus. Prospectively collected stool specimens (n ‫؍‬ 1,556) were evaluated using the BioFire FilmArray GI Panel and tested with conventional stool culture and molecular methods for comparison. The FilmArray GI Panel sensitivity was 100% for 12/22 targets and >94.5% for an additional 7/22 targets. For the remaining three targets, sensitivity could not be calculated due to the low prevalences in this study. The FilmArray GI Panel specificity was >97.1% for all panel targets. The FilmArray GI Panel provides a comprehensive, rapid, and streamlined alternative to conventional methods for the etiologic diagnosis of infectious gastroenteritis in the laboratory setting. The potential advantages include improved performance parameters, a more extensive menu of pathogens, and a turnaround time of as short as 1 h. Infectious gastroenteritis (IGE) is a leading cause of global morbidity and mortality. It is estimated that IGE contributes to the death of 2,195 children each day (1). IGE also contributes to serious morbidities, such as malnutrition, stunting, and impaired cognitive function (2, 3). Diarrheal disease disproportionately affects developing nations, but IGE remains a significant problem in industrialized countries as well. For example, it is estimated that each year, approximately 178.8 million cases of gastrointestinal illness occur in the United States, resulting in 474,000 hospitalizations and 5,000 deaths (4). Although the etiologic agents responsible for about 80% of these illnesses are unidentified or otherwise unspecified (4), norovirus and Salmonella spp. are currently the most commonly identified pathogens associated with food-borne disease in the United States and account for 5.5 and 1.0 million cases each year, resp...

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Section: Discussionmentioning

confidence: 99%

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

et al. 2015

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“…In hospitalised children aged 6-24 months, rotaviruses are a major cause of gastroenteritis and it has been shown that 24-35% of hospitalisations of gastroenteritis were attributable to rotaviruses [Lew et al, 1990;Monroe et al, 1991]. Several studies have characterised the group A rotaviruses circulating in the paediatric population in hospitals and in the community [Cubitt et al, 2000;Iturriza-Gomara et al, 2000;Koshimura et al, 2000] by G and P typing and electropherotyping [Noel et al, 1991].…”

Section: Introductionmentioning

confidence: 99%

Diversity of enteric viruses detected in patients with gastroenteritis in a tertiary referral paediatric hospital

Gallimore¹,

Cubitt

Richards³

et al. 2004

Journal of Medical Virology

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The genetic diversity of enteric viruses co-circulating in a cohort of patients with viral gastroenteritis in a large tertiary paediatric hospital in London, UK, was determined. Multiple strains of noroviruses (NV), sapoviruses (SV) and astroviruses (HAsV) were detected in these patients, indicating the likelihood of multiple introductions from different sources, possible sub-clinical infections and simultaneous infection with different viruses in immunocompromised and other patients. Routine screening of immunocompromised patients and infection control procedures are important to prevent nosocomial infection.

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“…In the USA, rotavirus infection accounts for 48% of viral gastroenteritis and 56 000 hospitalisations per year, 2 while in developing countries 600 000 children die from rotavirus each year. 3 Despite the development of enzyme linked immunosorbent assays (ELISAs) and molecular techniques to determine the aetiology of infectious diarrhoea, in most cases there is a delay of several days between the collection of a stool sample and a microbiological diagnosis. Indeed, a delay of 8.2 days has been reported recently.…”

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confidence: 99%

A novel method for rapidly diagnosing the causes of diarrhoea

Probert

Jones²,

Ratcliffe³

2004

Gut

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Background: The microbiological diagnosis of infectious diarrhoea may take several days using conventional techniques. In order to determine whether flatus can be used to make a rapid diagnosis, the volatile organic compounds associated with diarrhoea were analysed. Methods: Stool samples were collected from 35 patients with infectious diarrhoea and from six healthy controls. Gaseous compounds were extracted from a headspace using solid phase microextraction and analysed using gas chromatography and mass spectroscopy. Results: Characteristic patterns of volatile gases were found for the main causes of infectious diarrhoea in hospitals. Furan species without indoles indicated Clostridium difficile, ethyl dodecanoate indicated rotavirus, ammonia without ethyl dodecanoate suggested other enteric viruses, and the absence of hydrocarbons and terpenes indicated Campylobacter infection. Conclusion: These results could be the basis of rapid near patient diagnosis of infectious diarrhoea.

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Six-year retrospective surveillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada

Cited by 63 publications

References 0 publications

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

Multicenter Evaluation of the BioFire FilmArray Gastrointestinal Panel for Etiologic Diagnosis of Infectious Gastroenteritis

Diversity of enteric viruses detected in patients with gastroenteritis in a tertiary referral paediatric hospital

A novel method for rapidly diagnosing the causes of diarrhoea

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