Size and shape effects in the biodistribution of intravascularly injected particles

Decuzzi, Paolo; Godin, Biana; Tanaka, Takemi; Lee, Sei Young; Chiappini, Ciro; Liu, X.; Ferrari, Mauro

doi:10.1016/j.jconrel.2009.10.014

Cited by 857 publications

(636 citation statements)

References 41 publications

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“…32 Another approach to improve nanoparticle transcytosis is to modify the nanoparticles themselves, such as modification of size, shape, and surface. 33,34 By changing nanoparticle properties and design, the profile of proteins adsorbed to their surfaces from the surrounding environment, such as for instance blood serum, is also changed. 35,36 It has been hypothesized that this layer (corona) of proteins on the nanoparticle surface affects much of the interactions with cells 37,38 and thus properties of the bare nanoparticle surface, such as charge, are less important for cellular interactions.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle accumulation and transcytosis in brain endothelial cell layers

et al. 2013

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The Blood-Brain Barrier (BBB) is a selective barrier, which controls and limits access to the central nervous system (CNS). The selectivity of the BBB relies on specialized characteristics of the endothelial cells that line the microvasculature, including the expression of intercellular tight junctions, which limit paracellular permeability. Several reports suggest that nanoparticles have a unique capacity to cross the BBB. However, direct evidence of nanoparticle transcytosis is difficult to obtain, and we found that typical transport studies present several limitations when applied to nanoparticles. In order to investigate the capacity of nanoparticles to access and transport across the BBB, several different nanomaterials, including silica, titania and albumin-or transferrinconjugated gold nanoparticles of different sizes, were exposed to a human in vitro BBB model of endothelial hCMEC/D3 cells. Extensive transmission electron microscopy imaging was applied in order to describe nanoparticle endocytosis and typical intracellular localisation, as well as to look for evidence of eventual transcytosis. Our results show that all of the nanoparticles were internalised, to different extents, by the BBB model and accumulated along the endo-lysosomal pathway. Rare events suggestive of nanoparticle transcytosis were also observed for several of the tested materials.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle accumulation and transcytosis in brain endothelial cell layers

et al. 2013

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“…While it is clear that nanoparticle size and shape are key factors for uptake [15][16][17] , current targeting strategies also involve conjugation of biofunctional moieties to nanoparticles in an effort to determine the biological outcomes 1,2,9,12,[18][19][20] . In some cases this seems successful, 3 but in others less so (for example targeting moieties can hinder biological barrier penetration 8,11 ).…”

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confidence: 99%

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface

et al. 2013

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“…5 Since this corona alters their outer surface, it can influence the effective nanoparticle behavior. 6 Apart from their surface properties, a crucial parameter is the effective size of nanoparticles in the blood circulation because it directly influences their clearance and biodistribution, [7][8][9][10] as well as their subsequent uptake and processing by target cells. 11,12 Furthermore, it is suggested that the effective size of nanoparticles might be related to their toxicity.…”

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confidence: 99%

Sizing Nanomatter in Biological Fluids by Fluorescence Single Particle Tracking

Braeckmans¹,

Buyens²,

Bouquet³

et al. 2010

Nano Lett.

142

104

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Accurate sizing of nanoparticles in biological media is important for drug delivery and biomedical imaging applications since size directly influences the nanoparticle processing and nanotoxicity in vivo. Using fluorescence single particle tracking we have succeeded for the first time in following the aggregation of drug delivery nanoparticles in real time in undiluted whole blood. We demonstrate that, by using a suitable surface functionalization, nanoparticle aggregation in the blood circulation is prevented to a large extent.

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Size and shape effects in the biodistribution of intravascularly injected particles

Cited by 857 publications

References 41 publications

Nanoparticle accumulation and transcytosis in brain endothelial cell layers

Nanoparticle accumulation and transcytosis in brain endothelial cell layers

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface

Sizing Nanomatter in Biological Fluids by Fluorescence Single Particle Tracking

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