Size and Stability of Reconstituted Sesame Oil Bodies

Peng, Ching‐I; Lin, I-Ping; Lin, Ching-Kuan; Tzen, Jason T.C.

doi:10.1021/bp034129z

Cited by 87 publications

(77 citation statements)

References 37 publications

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“…The sizes of artificial oil bodies could be controlled by changing the ratio of triacylglycerol over oil-body protein, whereas both thermostability and structural stability of artificial oil bodies decreased as their size increased, and vice versa [87]. For encapsulation of artificial oil bodies, recombinant oleosins expressed in Escherichia coli were found comparable to native oleosins isolated from seed oil bodies [141,142].…”

Section: Artificial Oil Bodies In Basic Research Studiesmentioning

confidence: 99%

“…It is demonstrated that the structural role of oleosin also protects Arabidopsis thaliana seeds against freeze/thaw-induced damage of their cells in vivo [82]. The contents of oleosins are found to determine sizes of seed oil bodies; presumably the ratio of oleosin over triacylglycerol is inversely proportional to the sizes of oil bodies [83][84][85][86][87][88]. The stability of isolated oil bodies could be substantially enhanced after their surface proteins were cross-linked by linker molecules, such as glutaraldehyde or genipin [87].…”

Section: Proposed Functions Of Oleosinmentioning

confidence: 99%

“…The contents of oleosins are found to determine sizes of seed oil bodies; presumably the ratio of oleosin over triacylglycerol is inversely proportional to the sizes of oil bodies [83][84][85][86][87][88]. The stability of isolated oil bodies could be substantially enhanced after their surface proteins were cross-linked by linker molecules, such as glutaraldehyde or genipin [87]. Being structural proteins, oleosin isoforms as well as caleosin ones are partially degraded by a special thiolprotease, thioredoxin h, after germination, and this specific degradation of oil-body structural proteins is proposed to be associated with mobilization of oil bodies in seedlings [89][90][91][92].…”

Section: Proposed Functions Of Oleosinmentioning

confidence: 99%

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Integral Proteins in Plant Oil Bodies

Tzen

2012

ISRN Botany

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Hydrophobic storage neutral lipids are stably preserved in specialized organelles termed oil bodies in the aqueous cytosolic compartment of plant cells via encapsulation with surfactant molecules including phospholipids and integral proteins. To date, three classes of integral proteins, termed oleosin, caleosin, and steroleosin, have been identified in oil bodies of angiosperm seeds. Proposed structures, targeting traffic routes, and biological functions of these three integral oil-body proteins were summarized and discussed. In the viewpoint of evolution, isoforms of oleosin and caleosin are found in oil bodies of pollens as well as those of more primitive species; moreover, caleosin-and steroleosin-like proteins are also present in other subcellular locations besides oil bodies. Technically, artificial oil bodies of structural stability similar to native ones were successfully constituted and seemed to serve as a useful tool for both basic research studies and biotechnological applications.

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Section: Artificial Oil Bodies In Basic Research Studiesmentioning

confidence: 99%

Section: Proposed Functions Of Oleosinmentioning

confidence: 99%

Section: Proposed Functions Of Oleosinmentioning

confidence: 99%

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Integral Proteins in Plant Oil Bodies

Tzen

2012

ISRN Botany

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“…Compared with oil bodies, which are generally thought to be immobile in plant cells (27), peroxisomes are largely mobile and occasionally move across the cytoplasm in a microtubule-and actin filament-dependent manner in animals and plants, respectively (28 -30). Direct membrane associations between oil bodies and peroxisomes have been detected in mammalian cells, yeast, and plants (31)(32)(33).…”

mentioning

confidence: 99%

Sucrose Production Mediated by Lipid Metabolism Suppresses the Physical Interaction of Peroxisomes and Oil Bodies during Germination of Arabidopsis thaliana

Cui

Hayashi

Otomo

et al. 2016

Journal of Biological Chemistry

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Physical interaction between organelles is a flexible event and essential for cells to adapt rapidly to environmental stimuli. Germinating plants utilize oil bodies and peroxisomes to mobilize storage lipids for the generation of sucrose as the main energy source. Although membrane interaction between oil bodies and peroxisomes has been widely observed, its underlying molecular mechanism is largely unknown. Here we present genetic evidence for control of the physical interaction between oil bodies and peroxisomes. We identified alleles of the sdp1 mutant altered in oil body morphology. This mutant accumulates bigger and more oil body aggregates compared with the wild type and showed defects in lipid mobilization during germination. SUGAR DEPENDENT 1 (SDP1) encodes major triacylglycerol lipase in Arabidopsis. Interestingly, sdp1 seedlings show enhanced physical interaction between oil bodies and peroxisomes compared with the wild type, whereas exogenous sucrose supplementation greatly suppresses the interaction. The same phenomenon occurs in the peroxisomal defective 1 (ped1) mutant, defective in lipid mobilization because of impaired peroxisomal ␤-oxidation, indicating that sucrose production is a key factor for oil body-peroxisomal dissociation. Peroxisomal dissociation and subsequent release from oil bodies is dependent on actin filaments. We also show that a peroxisomal ATP binding cassette transporter, PED3, is the potential anchor protein to the membranes of these organelles. Our results provide novel components linking lipid metabolism and oil body-peroxisome interaction whereby sucrose may act as a negative signal for the interaction of oil bodies and peroxisomes to fine-tune lipolysis.

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“…An alternative way to stabilize LDs may be to cross link the amphipathic arms of the oleosins; this has already been demonstrated in vitro by the chemical cross linking of artificial LDs with either gluteraldehyde or genipin (Peng et al, 2003). Recently, the sesame seed (Sesamum indicum) H-oleosin but not the L-oleosin was found to be ubiquitinated shortly after germination, and two of the three confirmed ubiquitination sites were located in the H-domain (Hsiao and Tzen, 2011).…”

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confidence: 99%

In Vivo Packaging of Triacylglycerols Enhances Arabidopsis Leaf Biomass and Energy Density

et al. 2013

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Our dependency on reduced carbon for energy has led to a rapid increase in the search for sustainable alternatives and a call to focus on energy densification and increasing biomass yields. In this study, we generated a uniquely stabilized plant structural protein (cysteine [Cys]-oleosin) that encapsulates triacylglycerol (TAG). When coexpressed with diacylglycerol O-acyltransferase (DGAT1) in Arabidopsis (Arabidopsis thaliana), we observed a 24% increase in the carbon dioxide (CO 2 ) assimilation rate per unit of leaf area and a 50% increase in leaf biomass as well as approximately 2-, 3-, and 5-fold increases in the fatty acid content of the mature leaves, senescing leaves, and roots, respectively. We propose that the coexpression led to the formation of enduring lipid droplets that prevented the futile cycle of TAG biosynthesis/lipolysis and instead created a sustained demand for de novo lipid biosynthesis, which in turn elevated CO 2 recycling in the chloroplast. Fatty acid profile analysis indicated that the formation of TAG involved acyl cycling in Arabidopsis leaves and roots. We also demonstrate that the combination of Cys-oleosin and DGAT1 resulted in the highest accumulation of fatty acids in the model single-cell eukaryote, Saccharomyces cerevisiae. Our results support the notion that the prevention of lipolysis is vital to enabling TAG accumulation in vegetative tissues and confirm the earlier speculation that elevating fatty acid biosynthesis in the leaf would lead to an increase in CO 2 assimilation. The Cysoleosins have applications in biofuels, animal feed, and human nutrition as well as in providing a tool for investigating fatty acid biosynthesis and catabolism.

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Size and Stability of Reconstituted Sesame Oil Bodies

Cited by 87 publications

References 37 publications

Integral Proteins in Plant Oil Bodies

Integral Proteins in Plant Oil Bodies

Sucrose Production Mediated by Lipid Metabolism Suppresses the Physical Interaction of Peroxisomes and Oil Bodies during Germination of Arabidopsis thaliana

In Vivo Packaging of Triacylglycerols Enhances Arabidopsis Leaf Biomass and Energy Density

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