Size control of calcium alginate beads containing living cells using micro-nozzle array

Sugiura, Shinji; Oda, Tatsuya; Izumida, Yasuhiko; Aoyagi, Yasuyuki; Satake, Mitsuo; Ochiai, Atsushi; Ohkohchi, Nobuhiro; Nakajima, Mitsutoshi

doi:10.1016/j.biomaterials.2004.08.029

Cited by 299 publications

(219 citation statements)

References 19 publications

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“…Moreover, researchers developed microchannel emulsification for which every hole (channel) for discrete phase droplet formation is custom made. Silicon [115][116][117][118], metal [119][120] and polymer [121][122] based highly uniform microchannels have been used for monodisperse (CV <5%) particle manufacture [123]. The difference between membrane and microchannel emulsification is the fabrication of the emulsification material, which in turn affects the pore size distribution.…”

Section: Membrane/microchannel Emulsification: Controlling the Droplementioning

confidence: 99%

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Gökmen

Prez

2012

Progress in Polymer Science

451

309

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Section: Membrane/microchannel Emulsification: Controlling the Droplementioning

confidence: 99%

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Gökmen

Prez

2012

Progress in Polymer Science

451

309

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“…Furthermore, microparticle suspensions allow effective diffusion of nutrients and oxygen to cells and efficient removal of metabolic waste products. To ensure optimal diffusive mass transport, diameter of microparticles should be maintained below 400µm [64] .…”

Section: Advanced Materials Electrosprayed Injectable Microcapsules Amentioning

confidence: 99%

Living Cell Factories ‐ Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery

et al. 2015

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Minimally invasive delivery of "living cell factories" consisting of cells and therapeutic agents has gained wide attention for next generation biomaterial device systems for multiple applications including musculoskeletal tissue regeneration, diabetes and cancer. Cellularbased microcapsules and microcarrier systems offer several attractive features for this particular purpose. One such technology capable of generating these types of systems is electrohydrodynamic (EHD) spraying. Depending on various parameters including applied voltage, biomaterial properties (viscosity, conductivity) and needle geometry, complex structures and arrangements can be fabricated for therapeutic strategies. In this paper, we outline the advances in the use of EHD technology specifically in the manipulation of bioactive and dynamic material systems to control size, composition and configuration in the development of minimally invasive micro-scaled biopolymeric systems. The exciting therapeutic applications of this technology, future perspectives and associated challenges are also presented.

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“…Current strategies used to form discrete hydrogel particles or droplets include electrostatic extrusion, 1, 2 acoustic emulsification, 3 microfluidic micro-nozzle array 4 and airflow nozzles, 5 to mention some. However, these techniques have limitations in terms of producing small (below 100µm) and monodisperse particles.…”

Section: Introductionmentioning

confidence: 99%

Versatile, cell and chip friendly method to gel alginate in microfluidic devices

et al. 2016

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Alginate is used extensively in microfluidic devices to produce discreet beads or fibres at the microscale. Such structures may be used to encapsulate sensitive cargoes such as cells and biomolecules. On chip gelation of alginate represents a significant challenge since gelling kinetics or physicochemical conditions are not biocompatible. Here we present a new method that offers a hitherto unprecedented level of control over the gelling kinetics and pH applied to the encapsulation of a variety of cells in both bead and fibre geometries. This versatile approach proved extremely simple to implement and resulted in highly reliable device operation and very high viability of several different encapsulated cell types. We believe this method offers a paradigm shift in alginate gelling technology for application in microfluidics.

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Size control of calcium alginate beads containing living cells using micro-nozzle array

Cited by 299 publications

References 19 publications

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Porous polymer particles—A comprehensive guide to synthesis, characterization, functionalization and applications

Living Cell Factories ‐ Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery

Versatile, cell and chip friendly method to gel alginate in microfluidic devices

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