Size-controllable Fe-N/C single-atom nanozyme with exceptional oxidase-like activity for sensitive detection of alkaline phosphatase

Chen, Qiumeng; Li, Siqi; Liu, Yuan; Zhang, Xiaodan; Tang, Yue; Chai, Hongxiang; Huang, Yuming

doi:10.1016/j.snb.2019.127511

Cited by 248 publications

(150 citation statements)

References 66 publications

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“…However, the precise mechanism of this catalytic activation of atmospheric oxygen is still under debate. Chen et al [3,19] The hydrogen peroxide reacting with the Fe 2+ /Fe 3+ in the Fe-N x -C structure can generate superoxide radicals. H 2 O 2 can also react with the Fe-N x centers and produce • OH by a Fenton-like reaction.…”

Section: Discussionmentioning

confidence: 99%

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

et al. 2020

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Carbon-based (nano)materials doped with transition metals, nitrogen and other heteroatoms are considered active heterogeneous catalysts in a wide range of chemical processes. Recently they have been scrutinized as artificial enzymes since they can catalyze proton-coupled electron transfer reactions vital for living organisms. Herein, interactions between Gram-positive and Gram-negative bacteria and either metal-free N and/or S doped or metal containing Fe−N−S co-doped porous carbons are studied. The Fe- and N-co-doped porous carbons (Fe−N−C) exhibit enhanced affinity toward bacteria as they show the highest adsorption capacity. Fe−N−C materials also show the strongest influence on the bacteria viability with visible toxic effect. Both types of bacteria studied reacted to the presence of Fe-doped carbons in a similar manner, showing a decrease in dehydrogenases activity in comparison to controls. The N-coordinated iron-doped carbons (Fe−N−C) may exhibit oxidase/peroxidase-like activity and activate O2 dissolved in the solution and/or oxygen-containing species released by the bacteria (e.g., H2O2) to yield highly bactericidal reactive oxygen species. As Fe/N/ and/or S-doped carbon materials efficiently adsorb bacteria exhibiting simultaneously antibacterial properties, they can be applied, inter alia, as microbiological filters with enhanced biofouling resistance.

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Section: Discussionmentioning

confidence: 99%

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

et al. 2020

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“…2b-d). [73][74][75][76] These technologies are important guarantees for SAzyme research. Size-controllable SAzymes with exceptional oxidase-mimic activity by pyrolysis of Fe-Zn bimetallic ZIFs were prepared by Chen et al 73 The oxidase-mimic activity could be regulated by changing the ratio of methanol to metal precursors and the calcination temperature.…”

Section: Structural Advantages Of Single-sitesmentioning

confidence: 99%

“…[73][74][75][76] These technologies are important guarantees for SAzyme research. Size-controllable SAzymes with exceptional oxidase-mimic activity by pyrolysis of Fe-Zn bimetallic ZIFs were prepared by Chen et al 73 The oxidase-mimic activity could be regulated by changing the ratio of methanol to metal precursors and the calcination temperature. Based on the synergistic effects of the degree of graphitization, particle sizedependent surface areas and exposure level of active sites, an optimal SAzyme was prepared with a molar ratio of methanol to Zn 2+ of 1320 : 1 and pyrolysis at 900 C. In natural sulte oxidase enzyme, Fe and Mo active sites work synergistically for sulte oxidation.…”

Section: Structural Advantages Of Single-sitesmentioning

confidence: 99%

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

et al. 2020

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“…used a facial pyrolysis method to synthesize a size‐controllable Fe‐N/C nanozyme with exclusive single Fe atoms coordinated Fe‐N x sites (Figure 5c). [ 32 ] The prepared Fe‐N/C SAzyme exhibited the exceptional high OXD‐like activity, which was able to catalyze oxidation of POD colorimetric substrates TMB, DAB or ABTS by dissolved oxygen to provide a color reaction (Figure 5d). They thus designed a novel colorimetric biosensor platform by employing Fe‐N/C SAzymes to detect alkaline phosphatase (ALP) and achieved highly sensitive detection with an ultra‐low limit of detection of 0.02 U/L.…”

Section: Types and Applications Of Sazymesmentioning

confidence: 99%

Advances in Single‐Atom Nanozymes Research^†

Jiang

Liang

2020

Chin. J. Chem.

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Nanozyme | Single-atom nanozyme | Biomedical application Nanozymes with intrinsic enzyme-like properties have attracted significant interest owing to their capability to address the limitations of traditional enzymes such as fragility, high cost and difficult mass production. However, the currently reported nanozymes are generally less active than natural enzymes. In recent years, with the rapid development of nanoscience and nanotechnology, single-atom nanozymes (SAzymes) with well-defined electronic and geometric structures have shown a promise to serve as direct surrogates of traditional enzymes by mimicking the highly evolved catalytic center of natural enzymes. In this review, we will introduce the enzymatic characteristics and recent advances of SAzymes, and summarize their significant applications from in vitro detection to in vivo monitoring and therapy.

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Size-controllable Fe-N/C single-atom nanozyme with exceptional oxidase-like activity for sensitive detection of alkaline phosphatase

Cited by 248 publications

References 66 publications

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

Advances in Single‐Atom Nanozymes Research^†

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Size-controllable Fe-N/C single-atom nanozyme with exceptional oxidase-like activity for sensitive detection of alkaline phosphatase

Cited by 248 publications

References 66 publications

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

Atomic engineering of single-atom nanozymes for enzyme-like catalysis

Advances in Single‐Atom Nanozymes Research†

Contact Info

Product

Resources

About

Advances in Single‐Atom Nanozymes Research^†