Size-controlled DNA-cross-linked hydrogel coated silica nanoparticles served as a ratiometric fluorescent probe for the detection of adenosine triphosphate in living cells

Ji, Xiaoting; Wang, Junning; Niu, Shuyan; Ding, Caifeng

doi:10.1039/c9cc01832h

Cited by 36 publications

(30 citation statements)

References 34 publications

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“…Radically improved features of the present hydrogel system have advantages over the so far published fluorescent hydrogel systems in the literature (Chang et al, 2009;Ji et al, 2019;Wu et al, 2019). The BBB model explicitly showed the experimental proof for the hypothesized concept of the hydrophobically modified nanogels using linseed oil-based polyol transmigrating across the tight junctions of BBB.…”

Section: Discussionmentioning

confidence: 55%

Development of Multifunctional Biopolymeric Auto-Fluorescent Micro- and Nanogels as a Platform for Biomedical Applications

Vashist

Atluri

Raymond

et al. 2020

Front. Bioeng. Biotechnol.

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The emerging field of theranostics for advanced healthcare has raised the demand for effective and safe delivery systems consisting of therapeutics and diagnostics agents in a single monarchy. This requires the development of multi-functional bio-polymeric systems for efficient image-guided therapeutics. This study reports the development of size-controlled (micro-to-nano) auto-fluorescent biopolymeric hydrogel particles of chitosan and hydroxyethyl cellulose (HEC) synthesized using water-in-oil emulsion polymerization technique. Sustainable resource linseed oil-based polyol is introduced as an element of hydrophobicity with an aim to facilitate their ability to traverse the blood-brain barrier (BBB). These nanogels are demonstrated to have salient features such as biocompatibility, stability, high cellular uptake by a variety of host cells, and ability to transmigrate across an in vitro BBB model. Interestingly, these unique nanogel particles exhibited auto-fluorescence at a wide range of wavelengths 450-780 nm on excitation at 405 nm whereas excitation at 710 nm gives emission at 810 nm. In conclusion, this study proposes the developed bio-polymeric fluorescent micro-and nano-gels as a potential theranostic tool for central nervous system (CNS) drug delivery and image-guided therapy.

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Section: Discussionmentioning

confidence: 55%

Development of Multifunctional Biopolymeric Auto-Fluorescent Micro- and Nanogels as a Platform for Biomedical Applications

Vashist

Atluri

Raymond

et al. 2020

Front. Bioeng. Biotechnol.

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“…Either the cross‐linking between the DNA building blocks and nanomaterials is formed via physical interaction, or the nanomaterials are encapsulated in the DNA hydrogel networks. The nanomaterials applied for the development of hybrid NAHs cover a variety of categories, including carbon‐based nanomaterials, such as GO, [ 76 , 77 ] carbon‐nanotubes, [ 78 , 79 ] carbon dots, [ 80 , 81 , 82 ] quantum dots, [ 83 ] magnetic beads, [ 84 , 85 , 86 , 87 , 88 ] liposome, [ 57 ] gold nanoparticles (AuNPs), [ 68 , 75 , 89 , 90 , 91 ] silica nanoparticles, [ 54 , 92 ] Laponite nanoparticles, [ 93 , 94 , 95 ] and photonic crystal (PC). [ 96 ] The interaction between DNA and nanomaterials reveals their multiple functional features, further enhancing the corresponding applications.…”

Section: Functional Units and Their Assembly Strategies For Nahsmentioning

confidence: 99%

“…[ 149 ] However, the high permeability can also facilitate sufficient contact with specific stimuli, permitting a rapid response in the smart DNA hydrogels. [ 92 ] Consequently, a technique was used by which a specific substance was added to induce a slight change in the microstructure of the hydrogel, adjusting its permeability to prepare a DNA hydrogel film, which was associated with the capillary behavior for the ultra‐trace detection of cocaine. [ 150 ]…”

Section: The Functional Features and Smart Strategies Of Nahsmentioning

confidence: 99%

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

et al. 2021

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Nucleic acid-based hydrogels that integrate intrinsic biological properties of nucleic acids and mechanical behavior of their advanced assemblies are appealing bioanalysis and biomedical studies for the development of new-generation smart biomaterials. It is inseparable from development and incorporation of novel structural and functional units. This review highlights different functional units of nucleic acids, polymers, and novel nanomaterials in the order of structures, properties, and functions, and their assembly strategies for the fabrication of nucleic acid-based hydrogels. Also, recent advances in the design of multifunctional and stimuli-responsive nucleic acid-based hydrogels in bioanalysis and biomedical science are discussed, focusing on the applications of customized hydrogels for emerging directions, including 3D cell cultivation and 3D bioprinting. Finally, the key challenge and future perspectives are outlined.

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“…However, CTCs are a very small population. In general, there are <10 CTCs/mL whereas there are approximately 5 × 10 9 normal cells present in the same volume of blood sample (65, 114,115). Therefore, a variety of materials have been recently investigated for sensitive catch and release of CTCs.…”

Section: Capture and Release Of Circulating Tumor Cells (Ctcs)mentioning

confidence: 99%

Design, Bioanalytical, and Biomedical Applications of Aptamer-Based Hydrogels

Wang

et al. 2020

Front. Med.

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Aptamers are special types of single-stranded DNA generated by a process called systematic evolution of ligands by exponential enrichment (SELEX). Due to significant advances in the chemical synthesis and biotechnological production, aptamers have gained considerable attention as versatile building blocks for the next generation of soft materials. Hydrogels are high water-retainable materials with a three-dimensional (3D) polymeric network. Aptamers, as a vital element, have greatly expanded the applications of hydrogels. Due to their biocompatibility, selective binding, and molecular recognition, aptamer-based hydrogels can be utilized for bioanalytical and biomedical applications. In this review, we focus on the latest strategies of aptamer-based hydrogels in bioanalytical and biomedical applications. We begin this review with an overview of the underlying design principles for the construction of aptamer-based hydrogels. Next, we will discuss some bioanalytical and biomedical applications of aptamer-based hydrogel including biosensing, target capture and release, logic devices, gene and cancer therapy. Finally, the recent progress of aptamer-based hydrogels is discussed, along with challenges and future perspectives.

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Size-controlled DNA-cross-linked hydrogel coated silica nanoparticles served as a ratiometric fluorescent probe for the detection of adenosine triphosphate in living cells

Abstract: A ratiometric fluorescent nanoprobe for adenosine triphosphate sensing in living cells, based on silica nanoparticles and a DNA-functionalized hybrid hydrogel.

Cited by 36 publications

References 34 publications

Development of Multifunctional Biopolymeric Auto-Fluorescent Micro- and Nanogels as a Platform for Biomedical Applications

Development of Multifunctional Biopolymeric Auto-Fluorescent Micro- and Nanogels as a Platform for Biomedical Applications

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

Design, Bioanalytical, and Biomedical Applications of Aptamer-Based Hydrogels

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