Size-controlled long-circulating PICsome as a ruler to measure critical cut-off disposition size into normal and tumor tissues

Abstract

Selective disposition of nanocarriers into target tissue is an essential issue in drug delivery. Critical size of nanocarriers (∼150 nm) discriminating the permeability into normal and tumor tissues was determined by the use of size-tunable, polyion complex hollow vesicles (PICsome) as a ruler.

A Membrane-integrated Microfluidic Device to Study Permeation of Nanoparticles through Straight Micropores toward Rational Design of Nanomedicines

“…Using the C values, vs can be estimated from Eq. (8). If a linear relationship is observed between vs and ΔP, Eq.…”

“…PICsomes have a tunable size ranging from 100 -400 nm with narrow size distribution, and have been reported to show prolonged blood circulation and excellent tumor accumulation in mice for 100-nm PICsomes due to the EPR effect. 8,23,28 Furthermore, 100-nm PICsomes can be used for MRI through encapsulation of superparamagnetic iron oxide nanoparticles, and have successfully been utilized in the detection of early-stage tumors. 29 Figure 5 shows typical microscopic images of microfluidic channels in permeation tests.…”

“…Cy3-labeled PICsome with a diameter of 101 ± 3 nm and polydispersity index of 0.055 ± 0.023 was prepared as previously described. 8,23 Briefly, PEG-PAsp, Cy3-labeled PEG-PAsp and Homo-P(Asp-AP) were dissolved in 10 mM phosphate buffer (PB, NaCl-free, pH 7.4) separately, with a polymer concentration of 1 mg mL -1 . The solutions of PEG-PAsp and Cy3-labeled PEG-PAsp were blended in a volume ratio of 4:1.…”

“…normal endothelial gaps, it is clear that the pores of tumor vascular walls increase the permeability. Additionally, we reported that nanoparticles with sizes less than 150 nm can selectively accumulate in tumors, 8 suggesting that nanoparticle size is also important for increased permeability.…”

Evaluation of Dispersibility in Liquid and AC Magnetization Properties of Polyion Complex-Coupled Magnetic Nanoparticles

“…Research into magnetic nanoparticles coated with polyion complex (PIC) organic materials is ongoing due to their wide clinical applicability [1][2][3] , and the sizes and characteristics of core-shell structures of PIC micelles have rendered them useful as carriers of oligonucleotides and plasmid DNA for human gene therapy 4) .…”