Size‐controlled preparation of nanosoy for potential biomedical applications

Abstract

Soy protein isolate nanoparticles (nanosoy) have significant applications in drug delivery, wound care systems and tissue engineering. We report an optimum technique named nanoprecipitation which enabled one-step formation of near-monodisperse nanosoy based on solvent displacement. Excellent control over protein aggregation was achieved, producing nanoparticles in the size range of 5-15 nm. The effect of various process parameters such as temperature, solvent/non-solvent ratio, crosslinker content and type of surfactant on the particle size was investigated. The structural and morphological features were analysed using dynamic light scattering, X-ray diffraction and high-resolution transmission electron microscopy. The morphological examination indicated that the particles formed were spherical in shape and further reduction in the average particle diameter was achieved by reducing the temperature and solvent/non-solvent ratio of the reaction medium. Surface charge and stability of nanosoy dispersions were analysed using zeta potential measurements. Further, ciprofloxacin release was monitored using nanosoy as a drug carrier. This technique provides an effective and straightforward approach offering considerable advantages in terms of economic, technical and environmental performance.

Development and characterization of nanosoy‐reinforced dextran nanocomposite membranes

“…From Figure , it can be observed that a remarkable control over nanoparticle size was achieved with variation in temperature, where 5–15 nm and 90–140 nm particles were obtained at 30 °C and 50 °C, respectively. Nanoparticles in the size range of 0.2–0.7 μm were obtained at 60 °C, and the unfolding of proteins was evident at temperatures over 60 °C . Thus, particle size and polydispersity index (PDI) were found to increase with an increase in temperature.…”

“…As described in the experimental section, the size‐controlled preparation of NS was carried out using a nanoprecipitation technique. A detailed examination of the influence of temperature, solvent/nonsolvent ratio, type of surfactant, and glyoxal crosslinking on the size and size distribution of NS was performed in our earlier studies . Briefly, a reaction temperature of 30 °C and solvent/nonsolvent ratio of 1:30 were optimized for the preparation of NS that acted as nanofiller for the dextran matrix in the present work.…”

“…The size‐controlled synthesis of NS has been reported in detail in our previous work . In brief, the nanoparticles were prepared using a nanoprecipitation technique, in which an aliquot of soy protein solution (solvent phase) was added in a dropwise manner to ethanol (nonsolvent phase) that allowed the precipitation of nanosoy particles in the solution, which were separated by centrifugation.…”

Designing of smart nanogels based on tragacanth gum for cisplatin delivery

“…The release behavior of CP from the GTG‐LC nanogels was analyzed as per previous reports 22, 25 . Approximately 10 mg of CP‐loaded GTG‐LC nanogels was dispersed in 20 mL of PBS for monitoring the release behavior.…”

“…In vitro CP release study The release behavior of CP from the GTG-LC nanogels was analyzed as per previous reports. 22,25 Approximately 10 mg of CP-loaded GTG-LC nanogels was dispersed in 20 mL of PBS for monitoring the release behavior. Drug release from nanogels was observed at different pH (2.5 and 6.5) at 37 °C for 0.5-24 h. The absorption maxima of the supernatant containing released CP were measured at a wavelength of 270 nm using a Shimadzu UV-2450 spectrophotometer against PBS as blank.…”