Size dependent anti-invasiveness of silver nanoparticles in lung cancer cells

Que, Yu Mei; Fan, Xiao Qing; Lin, Xiao Juan; Jiang, Xiao Li; Hu, Ping; Tong, Xiaoyong; Tan, Qiaoyan

doi:10.1039/c9ra03662h

Cited by 20 publications

(8 citation statements)

References 35 publications

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“…Many researchers emphasize on cytotoxicity of the metal NPs and highlight the migration of tumor cell and metastasis-related ability may be impacted by nanomaterials 78 . Herein, the strong inhibition efficacy of AgNPs on migration was observed in cancer cells, which were in line with other group 79 , 80 . Thus, this suggests that silver nanoparticles may have potential function in the inhibition of the metastasis.…”

Section: Resultssupporting

confidence: 88%

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Skóra

Krajewska

Nowak

et al. 2021

Sci Rep

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Drug-resistance of bacteria is an ongoing problem in hospital treatment. The main mechanism of bacterial virulency in human infections is based on their adhesion ability and biofilm formation. Many approaches have been invented to overcome this problem, i.e. treatment with antibacterial biomolecules, which have some limitations e.g. enzymatic degradation and short shelf stability. Silver nanoparticles (AgNPs) may be alternative to these strategies due to their unique and high antibacterial properties. Herein, we report on yeast Saccharomyces cerevisiae extracellular-based synthesis of AgNPs. Transmission electron microscopy (TEM) revealed the morphology and structure of the metallic nanoparticles, which showed a uniform distribution and good colloid stability, measured by hydrodynamic light scattering (DLS). The energy dispersive X-ray spectroscopy (EDS) of NPs confirms the presence of silver and showed that sulfur-rich compounds act as a capping agent being adsorbed on the surface of AgNPs. Antimicrobial tests showed that AgNPs inhibit the bacteria growth, while have no impact on fungi growth. Moreover, tested NPs was characterized by high inhibitory potential of bacteria biofilm formation but also eradication of established biofilms. The cytotoxic effect of the NPs on four mammalian normal and cancer cell lines was tested through the metabolic activity, cell viability and wound-healing assays. Last, but not least, ability to deep penetration of the silver colloid to the root canal was imaged by scanning electron microscopy (SEM) to show its potential as the material for root-end filling.

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Section: Resultssupporting

confidence: 88%

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Skóra

Krajewska

Nowak

et al. 2021

Sci Rep

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“…The inhibitive effect of AgNPs decrease with the increase of their particle size and reversible for concentration. 65,66 In our study, as compared to CMBC-EDA/CHO-PECTIN, CMBC-EDA/CHO-PECTIN/AgNPs showed higher A549 cell proliferation and migration during the wound healing process (Figure 10). The wound healing in A549 cells might be due to the inductive effect of the synthesized film or the absence of inhibitory effect in AgNPs due to particle size and concentration.…”

Section: ■ Results and Discussionmentioning

confidence: 60%

AgNPs Ornamented Modified Bacterial Cellulose Based Self-Healable L-B-L Assembly via a Schiff Base Reaction: A Potential Wound Healing Patch

Khamrai

Banerjee

Paul

et al. 2020

ACS Appl. Bio Mater.

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A layer-by-layer (L-B-L) bacterial cellulose (BC)-based transdermal patch has been prepared via a Schiff base reaction. The L-B-L assembly consisting of covalently cross-linked ethylene diamine-modified carboxymethylated BC isolated from the Glucanoacetobacter xylinus (MTCC7795) bacterial strain and aldehydemodified pectin formed via a Schiff base reaction. The presence of the imine bond assists the self-healing process after being scratched in the presence of a pH 7.4 buffer solution monitored via optical microscopy, atomic force microscopy, and tensile strength analyses. The formation of the L-B-L assembly was confirmed using field-emission scanning electron microscopy (FESEM) analysis. Simultaneously, water swelling and deswelling studies were carried out to test its water retention efficiency. The presence of silver nanoparticles (AgNPs) has been confirmed by ultraviolet−visible spectroscopy and FESEM analyses. The antimicrobial activity of the AgNPs-incorporated transdermal patch has been examined over Staphylococcus aureus and Escherichia coli using the zone of inhibition method. Additionally, the cell viability assay was performed using the fluorescent dyes 4′,6-diamidino-2-phenylindole and propidium iodide. The AgNPs in the L-B-L assembly showed antimicrobial property against both types of bacteria. The cytotoxicity and wound healing property of the patch system have been studied over NIH 3T3 fibroblast and A549 epithelial cell lines. The L-B-L film also influenced the wound healing process of these two cell lines.

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“…AgNPs (13 nm) caused a decrease in the anti-apoptotic protein Bcl-2 and an increase in the pro-apoptotic protein Bax and upregulated the phosphorylation of NF-κB in A549 cells, indicating that AgNPs (13 nm) induced apoptosis via the NF-κB pathway. However, large AgNPs (45 and 92 nm) did not activate the apoptosis signaling pathway [166]. The size of silica NPs (19, 43, 68 nm) is negatively correlated with the apoptosis and necrosis rates of HepG2 cells [61].…”

Section: The Impact Of Np Physicochemical Properties On Apoptosismentioning

confidence: 95%

The Rational Design and Biological Mechanisms of Nanoradiosensitizers

2020

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Radiotherapy (RT) has been widely used for cancer treatment. However, the intrinsic drawbacks of RT, such as radiotoxicity in normal tissues and tumor radioresistance, promoted the development of radiosensitizers. To date, various kinds of nanoparticles have been found to act as radiosensitizers in cancer radiotherapy. This review focuses on the current state of nanoradiosensitizers, especially the related biological mechanisms, and the key design strategies for generating nanoradiosensitizers. The regulation of oxidative stress, DNA damage, the cell cycle, autophagy and apoptosis by nanoradiosensitizers in vitro and in vivo is highlighted, which may guide the rational design of therapeutics for tumor radiosensitization.

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Size dependent anti-invasiveness of silver nanoparticles in lung cancer cells

Abstract: Size-dependent anti-invasiveness effect of AgNPs was determined using A549 lung adenocarcinoma cells. The 13 nm AgNPs can significantly inhibit the migration and invasion of A549 cells and induce the elevated level of ROS and NF-κB directed cell apoptosis.

Cited by 20 publications

References 35 publications

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

Noncytotoxic silver nanoparticles as a new antimicrobial strategy

AgNPs Ornamented Modified Bacterial Cellulose Based Self-Healable L-B-L Assembly via a Schiff Base Reaction: A Potential Wound Healing Patch

The Rational Design and Biological Mechanisms of Nanoradiosensitizers

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