Size-Dependent Cytotoxicity and Reactive Oxygen Species of Cerium Oxide Nanoparticles in Human Retinal Pigment Epithelia Cells

Ma, Yuanyuan; Zhao, Laien; Liu, Jia; Jin, Yu; Huang, Yanmei; Zhu, Yuting; Li, Zelin; Zhao, Ruikang; Shi, Hua; Zhu, Yanping; Zhang, Zhuhong

doi:10.2147/ijn.s305676

Cited by 22 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cellular ATP levels were calculated by comparing the luminescence intensity of the treated cells to that of the vehicle control. The cytotoxicity of SiO 2 NPs was assessed using the LDH Release Assay (Beyotime, Beijing, China) as our previous method [ 34 ]. The absorbance was detected at a wavelength of 490 nm by using a Synergy H4 Hybrid microplate reader and data are presented as relative values compared to control.…”

Section: Methodsmentioning

confidence: 99%

“…The SiO 2 NP-induced changes in mitochondrial membrane potential were assessed as previously described [ 34 ]. Briefly, R28 cells were seeded in dishes at a density of 1 × 10 5 cells/mL and cultured overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Intracellular ROS production was assessed using H 2 DCF-DA staining as previously described [ 34 ]. Briefly, R28 cells were treated with 10 μM H 2 DCF-DA for 30 min in the cell culture incubator.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Zhang

Zhao

et al. 2022

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

Background Silica nanoparticles (SiO2 NPs) are extensively applied in the biomedical field. The increasing medical application of SiO2 NPs has raised concerns about their safety. However, studies on SiO2 NP-induced retinal toxicity are lacking. Methods We investigated the retinal toxicity of SiO2 NPs with different sizes (15 and 50 nm) in vitro and in vivo along with the underlying mechanisms. The cytotoxicity of SiO2 NPs with different sizes was assessed in R28 human retinal precursor cells by determining the ATP content and LDH release. The cell morphologies and nanoparticle distributions in the cells were analyzed by phase-contrast microscopy and transmission electron microscopy, respectively. The mitochondrial membrane potential was examined by confocal laser scanning microscopy. The retinal toxicity induced by SiO2 NPs in vivo was examined by immunohistochemical analysis. To further investigate the mechanism of retinal toxicity induced by SiO2 NPs, reactive oxygen species (ROS) generation, glial cell activation and inflammation were monitored. Results The 15-nm SiO2 NPs were found to have higher cytotoxicity than the larger NPs. Notably, the 15-nm SiO2 NPs induced retinal toxicity in vivo, as demonstrated by increased cell death in the retina, TUNEL-stained retinal cells, retinal ganglion cell degeneration, glial cell activation, and inflammation. In addition, The SiO2 NPs caused oxidative stress, as demonstrated by the increase in the ROS indicator H2DCF-DA. Furthermore, the pretreatment of R28 cells with N-acetylcysteine, an ROS scavenger, attenuated the ROS production and cytotoxicity induced by SiO2 NPs. Conclusions These results provide evidence that SiO2 NPs induce size-dependent retinal toxicity and suggest that glial cell activation and ROS generation contribute to this toxicity. Graphical Abstract

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Zhang

Zhao

et al. 2022

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…CeO 2 -NPs, as described above, have excellent antioxidant stress effects and have been shown to reduce photoinduced retinal degeneration. However, a recent study has shown that CeO 2 -NPs exerted toxic effects on human RPE cells (Ma, Li et al, 2021). The study showed that 15 nm CeO 2 -NPs at the concentration of 25 μg/ml reduced the viability of ARPE-19 cells, induced excessive ROS production, and caused mitochondrial dysfunction (Ma, Li et al, 2021).…”

Section: Cerium Oxide Nanoparticlesmentioning

confidence: 99%

“…However, a recent study has shown that CeO 2 -NPs exerted toxic effects on human RPE cells (Ma, Li et al, 2021). The study showed that 15 nm CeO 2 -NPs at the concentration of 25 μg/ml reduced the viability of ARPE-19 cells, induced excessive ROS production, and caused mitochondrial dysfunction (Ma, Li et al, 2021). In addition, the toxicity of CeO 2 -NPs coated with modified ethylene glycol (EGCNPs) on human lens epithelial cells also was evaluated by Hanafy et al (Hanafy, Cave et al, 2020).…”

Section: Cerium Oxide Nanoparticlesmentioning

confidence: 99%

Nanoparticles in ocular applications and their potential toxicity

Cao

Yang

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Nanotechnology has been developed rapidly in recent decades and widely applied in ocular disease therapy. Nano-drug delivery systems overcome the bottlenecks of current ophthalmic drug delivery and are characterized with strong biocompatibility, stability, efficiency, sustainability, controllability, and few side effects. Nanoparticles have been identified as a promising and generally safe ophthalmic drug-delivery system based on the toxicity assessment in animals. Previous studies have found that common nanoparticles can be toxic to the cornea, conjunctiva, and retina under certain conditions. Because of the species differences between humans and animals, advanced in vitro cell culture techniques, such as human organoids, can mimic the human organism to a certain extent, bringing nanoparticle toxicity assessment to a new stage. This review summarizes the advanced application of nanoparticles in ocular drug delivery and the potential toxicity, as well as some of the current challenges and future opportunities in nanotoxicological evaluation.

show abstract

Nanotechnology‐Mediated Immunomodulation Strategy for Inflammation Resolution

Li,

Liu,

Wang

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Inflammation serves as a common characteristic across a wide range of diseases and plays a vital role in maintaining homeostasis. Inflammation can lead to tissue damage and the onset of inflammatory diseases. Although significant progress is made in anti‐inflammation in recent years, the current clinical approaches mainly rely on the systemic administration of corticosteroids and antibiotics, which only provide short‐term relief. Recently, immunomodulatory approaches have emerged as promising strategies for facilitating the resolution of inflammation. Especially, the advanced nanosystems with unique biocompatibility and multifunctionality have provided an ideal platform for immunomodulation. In this review, the pathophysiology of inflammation and current therapeutic strategies are summarized. It is mainly focused on the nanomedicines that modulate the inflammatory signaling pathways, inflammatory cells, oxidative stress, and inflammation targeting. Finally, the challenges and opportunities of nanomaterials in addressing inflammation are also discussed. The nanotechnology‐mediated immunomodulation will open a new treatment strategy for inflammation therapy.

show abstract

Size-Dependent Cytotoxicity and Reactive Oxygen Species of Cerium Oxide Nanoparticles in Human Retinal Pigment Epithelia Cells

Cited by 22 publications

References 39 publications

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Nanoparticles in ocular applications and their potential toxicity

Nanotechnology‐Mediated Immunomodulation Strategy for Inflammation Resolution

Contact Info

Product

Resources

About