Size-dependent effects of polystyrene nanoplastics on autophagy response in human umbilical vein endothelial cells

Lu, Yanyang; Li, Heyang; Ren, Hongyun; Zhang, Xu; Huang, Fu-Yi; Zhang, Dandan; Huang, Qiansheng; Zhang, Xian

doi:10.1016/j.jhazmat.2021.126770

Cited by 116 publications

(45 citation statements)

References 47 publications

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“…The excitation and emission wavelengths of the fluorescently labeled PS particles were 535 and 610 nm, respectively. The dye (Nile red, about 10% w/w) was filled inside the whole plastic balls to make sure that the fluorescence could be stable for further experiments. , Depending on the product information provided by the manufacturer, the functional PS particles were synthesized through the microemulsion polymerization method. The amino-modified PS particles were synthesized using styrene and ammonium nitrate; the carboxyl-modified PS particles were synthesized using styrene and acrylic acid.…”

Section: Methodsmentioning

confidence: 99%

Cellular Process of Polystyrene Nanoparticles Entry into Wheat Roots

Zhu

Wang

Chen

et al. 2022

Environ. Sci. Technol.

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Nanoscale plastic particles are widely found in the terrestrial environment and being increasingly studied in recent years. However, the knowledge of their translocation and accumulation mechanism controlled by nanoplastic characterizations in plant tissues is limited, especially in plant cells. Here, 20 mg L–1 polystyrene nanoparticles (PS NPs) with different sizes and amino/carboxy groups were employed to investigate the internalization process in wheat roots and cells. From the results, we found that the uptake of small-size PS NPs in the root tissues was increased compared to that of large-size ones, but no PS NPs were observed in the vascular cylinder. Similar results were observed in their cellular uptake process. Besides, the cell wall could block the entry of large-size PS NPs while the cell membrane could not. The −NH2 group on the PS NPs surface could benefit their tissular/cellular translocation compared to the −COOH group. The internalization of PS NPs was controlled by both particle size and surface functional group, and the size should be the primary factor. Our findings offer important information for understanding the PS NPs behaviors in plant tissues, especially at the cellular level, and assessing their potential risk to food safety, quality, and agricultural sustainability.

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Section: Methodsmentioning

confidence: 99%

Cellular Process of Polystyrene Nanoparticles Entry into Wheat Roots

Zhu

Wang

Chen

et al. 2022

Environ. Sci. Technol.

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“…In this study, we consider the role that particles compatible with MPs may play in placental stress in terms of endoplasmic reticulum dilation/stressed mitochondria/oxidative stress. Numerous in vivo and in vitro studies have shown that MPs cause oxidative stress, apoptosis, inflammation, mitochondrial and lysosomal dysfunction, autophagy, and genotoxicity in various tissues (gut, intestine, liver) and cell types (human keratinocytes, human intestinal epithelial cells, gastric epithelial cells, human umbilical vein endothelial cells) [ 35 , 63 , 70 , 71 , 72 ]. Abnormal colocalization of mitochondria and endoplasmic reticulum has been also found in porcine oocytes exposed to Monobutyl phthalate (phthalates are a group of chemicals associated with PVC products as plasticizers in medical tubing, soft plastic toys, food packaging and in many personal care products) [ 73 ].…”

Section: Resultsmentioning

confidence: 99%

Deeply in Plasticenta: Presence of Microplastics in the Intracellular Compartment of Human Placentas

Ragusa

Matta

Cristiano

et al. 2022

IJERPH

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Microplastics (MPs) are defined as plastic particles smaller than 5 mm. They have been found almost everywhere they have been searched for and recent discoveries have also demonstrated their presence in human placenta, blood, meconium, and breastmilk, but their location and toxicity to humans have not been reported to date. The aim of this study was twofold: 1. To locate MPs within the intra/extracellular compartment in human placenta. 2. To understand whether their presence and location are associated with possible structural changes of cell organelles. Using variable pressure scanning electron microscopy and transmission electron microscopy, MPs have been localized in ten human placentas. In this study, we demonstrated for the first time the presence and localization in the cellular compartment of fragments compatible with MPs in the human placenta and we hypothesized a possible correlation between their presence and important ultrastructural alterations of some intracytoplasmic organelles (mitochondria and endoplasmic reticulum). These alterations have never been reported in normal healthy term pregnancies until today. They could be the result of a prolonged attempt to remove and destroy the plastic particles inside the placental tissue. The presence of virtually indestructible particles in term human placenta could contribute to the activation of pathological traits, such as oxidative stress, apoptosis, and inflammation, characteristic of metabolic disorders underlying obesity, diabetes, and metabolic syndrome and partially accounting for the recent epidemic of non-communicable diseases.

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Section: Introductionmentioning

confidence: 99%

“…A size-dependent induction by PS-NPs of autophagy initiation was observed recently in vitro in human umbilical vein endothelial cells (HUVECs) [ 35 ]. It was demonstrated that PS-NPs with a diameter of less than 100 nm could directly penetrate the cell membrane and aggregate in the cytoplasm [ 24 , 34 , 35 ]. In this study, 50 nm PS-NPs were chosen for treating human neuroblastoma SHSY-5Y cells, which is one of the most commonly used in vitro models in neurotoxicity studies [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

PS-NPs Induced Neurotoxic Effects in SHSY-5Y Cells via Autophagy Activation and Mitochondrial Dysfunction

Tang

Chen

et al. 2022

Brain Sciences

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Polystyrene nanoparticles (PS-NPs) are organic pollutants that are widely detected in the environment and organisms, posing potential threats to both ecosystems and human health. PS-NPs have been proven to penetrate the blood–brain barrier and increase the incidence of neurodegenerative diseases. However, information relating to the pathogenic molecular mechanism is still unclear. This study investigated the neurotoxicity and regulatory mechanisms of PS-NPs in human neuroblastoma SHSY-5Y cells. The results show that PS-NPs caused obvious mitochondrial damages, as evidenced by inhibited cell proliferation, increased lactate dehydrogenase release, stimulated oxidative stress responses, elevated Ca2+ level and apoptosis, and reduced mitochondrial membrane potential and adenosine triphosphate levels. The increased release of cytochrome c and the overexpression of apoptosis-related proteins apoptotic protease activating factor-1 (Apaf-1), cysteinyl aspartate specific proteinase-3 (caspase-3), and caspase-9 indicate the activation of the mitochondrial apoptosis pathway. In addition, the upregulation of autophagy markers light chain 3-II (LC3-II), Beclin-1, and autophagy-related protein (Atg) 5/12/16L suggests that PS-NPs could promote autophagy in SHSY-5Y cells. The RNA interference of Beclin-1 confirms the regulatory role of autophagy in PS-NP-induced neurotoxicity. The administration of antioxidant N-acetylcysteine (NAC) significantly attenuated the cytotoxicity and autophagy activation induced by PS-NP exposure. Generally, PS-NPs could induce neurotoxicity in SHSY-5Y cells via autophagy activation and mitochondria dysfunction, which was modulated by mitochondrial oxidative stress. Mitochondrial damages caused by oxidative stress could potentially be involved in the pathological mechanisms for PS-NP-induced neurodegenerative diseases.

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Size-dependent effects of polystyrene nanoplastics on autophagy response in human umbilical vein endothelial cells

Cited by 116 publications

References 47 publications

Cellular Process of Polystyrene Nanoparticles Entry into Wheat Roots

Cellular Process of Polystyrene Nanoparticles Entry into Wheat Roots

Deeply in Plasticenta: Presence of Microplastics in the Intracellular Compartment of Human Placentas

PS-NPs Induced Neurotoxic Effects in SHSY-5Y Cells via Autophagy Activation and Mitochondrial Dysfunction

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