Size dependent macrophage responses and toxicological effects of Ag nanoparticles

Park, Jeongsin; Lím, D.; Lim, Hyun Jeong; Kwon, Taejung; Choi, Jin Woo; Jeong, Sohee; Choi, In Hong; Cheon, Jinwoo

doi:10.1039/c1cc10357a

Cited by 218 publications

(147 citation statements)

References 25 publications

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“…Studies using various cell lines reported that Ag-NPs affected mitochondrial function and induced cell necrosis or apoptosis 24,25) . However, other investigators found no such cytotoxic effects of Ag-NPs on living cells 26) .…”

Section: Discussionmentioning

confidence: 99%

“…their size, size distribution, shape, state of dispersion, physical and chemical properties, surface area and porosity, surface chemistry) and by cell characteristics (e.g. cell line, number of cells per well, and the period of culture before and/or after exposure to nanoparticles) 17,[24][25][26]29,30) . A recent study showed a decrease in MC3T3-E1 cell viability after exposure to Ag-NPs using the WST-8 assay 31) , but this study used different test conditions for cell culture and exposure to Ag-NPs, which may explain the disparity between their results and ours.…”

Section: Discussionmentioning

confidence: 99%

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Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

et al. 2013

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With the continuous progress in nanomaterial development for biomedicine, the potential cytotoxicity of nanoparticles is drawing more attention and concern for clinical applications. The purpose of this study was to evaluate biological responses of new waterdispersible silver nanoparticles (Ag-NPs) stabilized by Ag-C σ-bonds in cultured murine macrophages (RAW264.7) and osteoblastlike cells (MC3T3-E1) using cell viability and morphological analyses. For RAW264.7, Ag-NPs seemed to induce cytotoxicity that was dependent on the Ag-NP concentration. However, no cytotoxic effects were observed in the MC3T3-E1 cell line. In microscopic analysis, Ag-NPs were taken up by MC3T3-E1 cells with only minor cell morphological changes, in contrast to RAW264.7 cells, in which particles aggregated in the cytoplasm and vesicles. The ability of endocytosis of macrophages may induce harmful effects due to expansion of cell vesicles compared to osteoblast-like cells with their lower uptake of Ag-NPs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

et al. 2013

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“…For example, phagocytosis has been believed for quite some time to be associated uniquely with the uptake of large particles (.1 mm). However, there are several reports that show the phagocytosis of nanometer-sized particles such as viruses (Clement et al 2006;Ghigo et al 2008), gold (Krpetic et al 2010;Franca et al 2011), silver (Park et al 2011), and polymer nanoparticles (Lunov et al 2011), as well as quantum dots .…”

Section: Sizementioning

confidence: 99%

Exploiting Endocytosis for Nanomedicines

Akinc

Battaglia

2013

Cold Spring Harbor Perspectives in Biology

192

169

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In this article, we briefly review the endocytic pathways used by cells, pointing out their defining characteristics and highlighting physical limitations that may direct the internalization of nanoparticles to a subset of these pathways. A more detailed description of these pathways is presented in the literature. We then focus on the endocytosis of nanomedicines and present how various nanomaterial parameters impact these endocytic processes. This topic is an area of active research, motivated by the recognition that an improved understanding of how nanomaterials interact at the molecular, cellular, and whole-organism level will lead to the design of better nanomedicines in the future. Next, we briefly review some of the important nanomedicines already on the market or in clinical development that serve to exemplify how endocytosis can be exploited for medical benefit. Finally, we present some key unanswered questions and remaining challenges to be addressed by the field.

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“…Aflatoxin producers, A. parasiticus and A. flavus are therefore the most characterized fungal models for studying mycotoxin biosynthesis. The 20nm AgNps are model silver nanoparticles that have been used consistently for toxicity assays in several models [9]. In a previous fungal toxicity study, 0.05mg/L concentrations of 25nm AgNps have demonstrated effective inhibition of fungal growth [7].…”

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confidence: 99%

Decreased Aflatoxin Biosynthesis Upon Uptake of 20 nm-sized Citrate Coated Silver Nanoparticles by the Aflatoxin producer Aspergillus parasiticus

et al. 2016

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The manufacture and usage of silver nanoparticles have drastically increased in recent years [1]. Hence, the feasibility of addition of high amounts of these nanoparticles into the environment through different routes has increased and is therefore a concern to environmental and public health [2]. Previous studies show that silver nanoparticles are toxic to various organisms: examples include toxicity against bacterial cells [3], aquatic plant cells [4], arthropods [5] and mammalian cells [6]. Recently toxicity of silver nanoparticles has also been demonstrated in fungal cells [7].In this study we examined the effect of 20nm sized citrate coated silver nanoparticles (cit-AgNp), synthesized and characterized (Fig. A) using previously established protocols [8], on growth and aflatoxin B1 biosynthesis in the filamentous fungus, Aspergillus parasiticus. Aflatoxin B1 is a mycotoxin and the most potent liver carcinogen known, associated with an estimated 25,200 and 155,000 liver cancer cases globally (Liu and Wu 2010), and hence its contamination in food and feed is a significant risk factor of liver cancer in humans and animals (CAST 2003 ; Liu and Wu 2010). Aflatoxin producers, A. parasiticus and A. flavus are therefore the most characterized fungal models for studying mycotoxin biosynthesis. The 20nm AgNps are model silver nanoparticles that have been used consistently for toxicity assays in several models [9]. In a previous fungal toxicity study, 0.05mg/L concentrations of 25nm AgNps have demonstrated effective inhibition of fungal growth [7]. Here we show that A. parasiticus cells successfully uptake cit-AgNp upon exposure at the concentration of 0.05mg/L. While time-course inductively coupled plasma optical emission spectrometry (ICP-OES) performed simultaneously on growth media and mycelia (Fig. B) convincingly demonstrated cit-AgNp uptake, the time course dry weight analysis of mycelia (Fig. C b) demonstrated no changes in growth rate, suggesting that at the applied concentration, uptake of cit-AgNp were not toxic to the fungal cells. Uptake of cit-AgNp however, resulted in a significant reduction of the aflatoxin B1 in growth medium (Fig C a) suggesting that although uptake of these particles do not result in gross changes of primary metabolism in this fungus, its metabolic machinery for aflatoxin biosynthesis (secondary metabolism) is inhibited significantly.Scanning electron micrographs and transmission electron micrographs of control mycelia and cit-AgNp up-taking mycelia (Fig. c i; ii) suggest that cit-AgNp localize predominantly at the cellular surface ( Fig. c ii (right side)) and associates with morphological fluctuations of the cell surface (Fig. c i (cit-AgNp treated A. parasiticus cell)). Overall, our findings implicate that while popular mycotoxin producers are robust to withstand and uptake silver nanoparticles, morphological fluctuations at their cell-environment interface during particle uptake can result in inhibition of mycotoxin biosynthesis and export into the environment [10].

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Size dependent macrophage responses and toxicological effects of Ag nanoparticles

Cited by 218 publications

References 25 publications

Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

Micromorphological cellular responses of MC3T3-E1 and RAW264.7 after exposure to water-dispersible silver nanoparticles stabilized by metal-carbon σ-bonds

Exploiting Endocytosis for Nanomedicines

Decreased Aflatoxin Biosynthesis Upon Uptake of 20 nm-sized Citrate Coated Silver Nanoparticles by the Aflatoxin producer Aspergillus parasiticus

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