Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells

Shi, Zhijun; Huang, Xin; Cai, Yurong; Tang, Ruikang; Yang, Disheng

doi:10.1016/j.actbio.2008.07.023

Cited by 419 publications

(288 citation statements)

References 32 publications

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“…4 Synthetic HAP crystals are now widely used in medical implants and as coatings on prostheses. 5 Compared to viral vectors, which possess risks of pathogenicity and immunogenicity, synthetic HAP nanoparticles have favourable biocompatibility and high osteoconductivity and/or osteoinductivity without immunogenicity or proinflammatory features. [6][7][8][9] HAP can not only directly inhibit the proliferation of cancer cells, 10 but also be used in a gene delivery system due to its safety, economy and efficiency.…”

Section: Introductionmentioning

confidence: 99%

Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo

Zhu¹,

Guo²,

Li³

et al. 2011

Asian J Androl

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DNA vector-based Stat3-specific RNA interference (si-Stat3) blocks Stat3 signalling and inhibits prostate tumour growth. However, the antitumour activity depends on the efficient delivery of si-Stat3. The effects on the growth of mouse prostate cancer cells of si-Stat3 delivered by hydroxyapatite were determined in this study. RM-1 tumour blocks were transplanted into C57BL/6 mice. CaCl 2 -modified hydroxyapatite carrying si-Stat3 plasmids were injected into tumours, and tumour growth and histology were determined. The expression levels of Stat3, pTyr-Stat3, Bcl-2, Bax, Caspase3, VEGF and cyclin D1 were measured by western blot analysis. Amounts of apoptosis in cancer cells were analysed with immunohistochemistry and the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assay. The results showed that hydroxyapatite-delivered si-Stat3 significantly suppressed tumour growth up to 74% (P,0.01). Stat3 expression was dramatically downregulated in the tumours. The immunohistochemistry and TUNEL results showed that si-Stat3-induced apoptosis (up to 42%, P,0.01). The Stat3 downstream genes Bcl-2, VEGF and cyclin D1 were also strongly downregulated in the tumour tissues that also displayed significant increases in Bax expression and Caspase3 activity. These results suggest that hydroxyapatite can be used for the in vivo delivery of plasmid-based siRNAs into tumours.

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Section: Introductionmentioning

confidence: 99%

Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo

Zhu¹,

Guo²,

Li³

et al. 2011

Asian J Androl

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“…Research into nanostructures and nano synthetic methods have discovered different nano forms of HAP with many properties such as different shapes, rods, tubes, plates and spheres [12][13][14]. Whilst Jun et al showed that HAP nanoparticles inhibit the growth of hepatic tumour cells and have stronger anti-tumour effect, Li et al demonstrated a beneficial effect of a nanocomposite of HAP and chitosan on human gastric cancer cells [15][16].…”

Section: Introductionmentioning

confidence: 99%

Effect of dilute gelatine on the ultrasonic thermally assisted synthesis of nano hydroxyapatite

Brundavanam

Jiang

Chapman

et al. 2011

Ultrasonics Sonochemistry

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Accepted ManuscriptThis is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Effect of Dilute Gelatine on the Ultrasonic Thermally AbstractA series of nano hydroxyapatite-gelatin composites with different dilute solutions of gelatin concentrations were synthesized by a thermally assisted low power ultrasonic irradiation method.The gelatine hydroxyapatite, (Gel-HAP) nanoparticles were prepared using Ca(NO 3 ) 2 and KH 2 PO 4 in the presence of gelatine in an aqueous solution. The synthesised products were heat treated between 100°C and 400°C. The effect of the addition of gelatin on the nucleation and growth of synthesised nano HAP was investigated. Characterisation was performed using X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FT-IR). The characterisation results indicate that gelatin has been appended to the nano HAP forming regular spherical shaped crystals of nano sized Gel-HAP.

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“…[8,9] Macropores on or inside the material can intensify the ingrowth of tissue. [39] Sufficient porosity, especially the presence of interconnected pores, determines the ability of bone graft materials to faster ingrowth and osteointegration. According to He et al, [40] pore sizes of at least 100 lm are sufficient for osteoid formation and ingrowth.…”

Section: B Microstructure Of the Sgg/peek Coatingsmentioning

confidence: 99%

Electrophoretic Deposition, Microstructure, and Corrosion Resistance of Porous Sol–Gel Glass/Polyetheretherketone Coatings on the Ti-13Nb-13Zr Alloy

Moskalewicz

Zych

Łukaszczyk

et al. 2017

Metall Mater Trans A

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In this study, microporous composite sol-gel glass/polyetheretherketone (SGG/PEEK) coatings were produced on the Ti-13Nb-13Zr titanium alloy by electrophoretic deposition. Coatings with different levels of high open porosity were developed by introducing SGG particles of varying diameters into the PEEK matrix. The microstructure of the coatings was characterized by electron microscopy and X-ray diffractometry. The coatings with 40-50 lm thickness were composed of semicrystalline SGG particles consisting of hydroxyapatite, CaSiO 3 , some Ca 2 SiO 4 , and an amorphous phase containing Ca, Si, P, and O, homogeneously embedded in a semicrystalline PEEK matrix. The size of SGG particles present in the coatings strongly influenced the formation of microcracks and their adhesion to the underlying substrate. Microscratch tests showed that the coating containing SGG particles with a diameter smaller than 45 lm and open porosity of 45 pct exhibited good adhesion to the titanium alloy substrate, much better than the coating containing particles with a diameter smaller than 85 lm and total open porosity equal to 48 pct. The corrosion resistance was investigated in Ringer's solution at a temperature of 310 K (37°C) for a pH equal to 7.4 and in deaerated solutions with the use of open-circuit potential, potentiodynamic polarization, and electrochemical impedance spectroscopy. The SGG/PEEK-coated alloy indicated better electrochemical corrosion resistance compared with the uncoated alloy.

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Size effect of hydroxyapatite nanoparticles on proliferation and apoptosis of osteoblast-like cells

Cited by 419 publications

References 32 publications

Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo

Plasmid-based Stat3 siRNA delivered by hydroxyapatite nanoparticles suppresses mouse prostate tumour growth in vivo

Effect of dilute gelatine on the ultrasonic thermally assisted synthesis of nano hydroxyapatite

Electrophoretic Deposition, Microstructure, and Corrosion Resistance of Porous Sol–Gel Glass/Polyetheretherketone Coatings on the Ti-13Nb-13Zr Alloy

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