Size Effect on Cell Uptake in Well‐Suspended, Uniform Mesoporous Silica Nanoparticles

Lu, Fang; Wu, Si Han; Hung, Yu-Chueh; Mou, Chung‐Yuan

doi:10.1002/smll.200900005

Cited by 948 publications

(751 citation statements)

References 38 publications

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“…1E indicates an increase of 90% in cellular uptake of 40 nm and 51% increase for 60nm AuNPs, during the subsequent 24 hours of incubation. An increase in size of the nanoparticles leads to an increment in the number of AuNPs taken up which is in accordance with other studies [30,[61][62][63]. These results reveal that cellular uptake is dependent on size and incubation time.…”

Section: Size-dependent Cellular Uptakesupporting

confidence: 92%

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Huefner

Septiadi

Wilts

et al. 2014

Methods

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Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies 2 pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.

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Section: Size-dependent Cellular Uptakesupporting

confidence: 92%

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Huefner

Septiadi

Wilts

et al. 2014

Methods

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“…For inorganic-based NPs, similar results were reported. Some researchers reported an optimal particle size for uptake of Au NPs, CNTs, and MSNs ($50 nm) [6,[39][40][41], but some others found smaller Au, silica, and IO NPs had improved cellular uptake than larger ones in a certain size range [42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

“…This could be caused by several vague parameters without clear and consistent definition. The first one is NP size, which is normally characterised as the Z-average hydrodynamic diameter (measured by light scattering technology) [29,41,50] or the number average size (measured and statistically calculated from TEM or SEM images) [6,28]. The second term is the dose of nanoparticle.…”

Section: Introductionmentioning

confidence: 99%

“…Some have used the mass concentration while others used the particle number concentration. The third one is the delivery efficiency, often expressed as the NP number [6,16,43,51], the mass amount (pg/cell) [16,41], or the relative amount of labelled fluorescence dye [48,52] taken up by each cell. In addition, the difference in the shape and surface property from a variety of materials and surface modified functional groups may also cause the inconsistency.…”

Section: Introductionmentioning

confidence: 99%

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Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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a b s t r a c tIt is well known that delivery efficiency to cells is highly dependent on particle size and the administered dose. However, there is a marked discrepancy in many reports, mainly due to the inconsistency in assessment of various parameters. In this particular research, we designed experiments using layered double hydroxide nanoparticles (LDH NPs) to specifically elucidate the effect of particle size, dose and dye loading manner on cellular uptake. Using the number of LDH NPs taken up by HCT-116 cells as the indicator of delivery efficiency, we found that (1) the size of sheet-like LDH in the range of 40-100 nm did not significantly affect their cellular uptake; (2) cellular uptake of 40 and 100 nm LDH NPs was increased proportionally to the number concentration below a critical value, but remained relatively constant beyond the critical value; and (3) the effect of the dye loading manner is mainly dependent on the loading capacity or yield. In particular, the loading capacity is determined by the NP specific surface area. This research may be extended to a larger size range to examine the size effect, but suggests that it is necessary to set up a protocol to evaluate the effects of NP's physicochemical properties on the cellular delivery efficiency.

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“…[1][2][3][4][5] The MSN materials with well-ordered cylindrical pore structures, such as MCM-41 and SBA-15, have attracted special interest in the biomedical field. 1 The large surface areas and pore volumes of these materials allow the efficient adsorption of a wide range of molecules, including small drugs, 6-10 therapeutic proteins, [11][12][13] antibiotics, 14,15 and antibodies.…”

mentioning

confidence: 99%

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

Zhao¹,

Sun²,

Zhang³

et al. 2011

ACS Nano

516

440

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The interactions of mesoporous silica nanoparticles (MSNs) of different particle sizes and surface properties with human red blood cell (RBC) membranes were investigated by membrane filtration, flow cytometry, and various microscopic techniques. Small MCM-41-type MSNs (∼100 nm) were found to adsorb to the surface of RBCs without disturbing the membrane or morphology. In contrast, adsorption of large SBA-15-type MSNs (∼600 nm) to RBCs induced a strong local membrane deformation leading to spiculation of RBCs, internalization of the particles, and eventual hemolysis. In addition, the relationship between the degree of MSN surface functionalization and the degree of its interaction with RBC, as well as the effect of RBC−MSN interaction on cellular deformability, were investigated. The results presented here provide a better understanding of the mechanisms of RBC−MSN interaction and the hemolytic activity of MSNs and will assist in the rational design of hemocompatible MSNs for intravenous drug delivery and in vivo imaging. R ecent advancements in particle size and morphology control of mesoporous materials have led to the creation of nano-and submicrometer-sized mesoporous silica nanoparticles (MSNs). [1][2][3][4][5] The MSN materials with well-ordered cylindrical pore structures, such as MCM-41 and SBA-15, have attracted special interest in the biomedical field. 1 The large surface areas and pore volumes of these materials allow the efficient adsorption of a wide range of molecules, including small drugs, 6-10 therapeutic proteins, 11-13 antibiotics, 14,15 and antibodies. 16 Therefore, these materials have been proposed for use as potential vehicles for biomedical imaging, real-time diagnosis, and controlled delivery of multiple therapeutic agents. [6][7][8]10,[17][18][19][20][21][22][23][24][25] Despite the considerable interest in the biomedical applications of MSNs, relatively few studies have been published on the biocompatibility of the two most common types of MSNs (MCM-41 and SBA-15). [26][27][28][29] Asefa and co-workers reported that the cellular bioenergetics (cellular respiration and ATP levels) were inhibited remarkably by large SBA-15 nanoparticles, but the inhibition was greatly reduced by smaller MCM-41-type nanoparticles. 26 These differences in the disruption of cellular bioenergetics are believed to be caused by the different surface areas, number of surface silanol groups, and/or particle sizes of both types of material. A recent study by Kohane and collaborators on the systemic effects of MCM-41 (particle size ∼150 nm) and SBA-15 (particle size ∼800 nm) MSNs in live animals revealed interesting findings regarding their biocompatibility. 27 While large doses of mesoporous silicas administered subcutaneously to mice appear to be relatively harmless, the same doses given intravenously or intraperitoneally were lethal. 27 A possible reason for the severe systemic toxicity of MSNs when injected intravenously could be the interactions of the nanoparticles with blood cells.Our initial studies...

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Size Effect on Cell Uptake in Well‐Suspended, Uniform Mesoporous Silica Nanoparticles

Cited by 948 publications

References 38 publications

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Gold nanoparticles explore cells: Cellular uptake and their use as intracellular probes

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Interaction of Mesoporous Silica Nanoparticles with Human Red Blood Cell Membranes: Size and Surface Effects

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