Size exclusion behavior of hydroxypropylcellulose beads with temperature-dependent porosity

Adrados, B.Palmarola; Galaev, Igor Yu.; Nilsson, Kersti B.; Mattìasson, Bo

doi:10.1016/s0021-9673(01)01142-6

Cited by 38 publications

(19 citation statements)

References 15 publications

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“…Many other authors have used thermo-responsive polymers for size exclusion [47] and affinity chromatography [48,49]. In addition to these applications, several types of polymers have been studied to develop a chromatography system.…”

Section: Discussionmentioning

confidence: 99%

Thermally responsive chromatographic materials using functional polymers

Kanazawa¹

2007

J of Separation Science

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Functional polymers that respond to small changes in environmental stimuli with large changes in their structure and properties are often called "intelligent" polymers. We have modified material surfaces with such polymers and used them in separation systems. Silica beads were modified with the temperature-responsive polymer poly(N-isopropylacrylamide) (PNIPAAm). PNIPAAm-grafted surfaces exhibited temperature-driven alterations of hydrophilic-hydrophobic surface-properties. Using this feature, PNIPAAm and related temperature-responsive polymers have been used to generate temperature-sensitive stationary phases for chromatographic separations. We attached several different functional polymers, including temperature- and pH-responsive polymers, to silica beads. These temperature-responsive stationary phases are useful in development of separation methods since adjusting the temperature represents an extra tool for optimizing the selectivity. Applications of thermally responsive columns for separations in the HPLC mode are demonstrated.

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Section: Discussionmentioning

confidence: 99%

Thermally responsive chromatographic materials using functional polymers

Kanazawa¹

2007

J of Separation Science

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“…Most of this has concentrated on exploiting the principle of thermally induced extension and collapse of polymer chains for modulating the fractionation range in size exclusion chromatography [11,12,14], hydrophobicity in HPLC [13,[15][16][17][18], and balancing electrostatic and hydrophobic interactions in ion exchange chromatography, through the use of copolymers containing both thermoresponsive and ion exchange components [19][20][21][22][23][24][25], rather than mitigating ligand masking and 'forced elution' in pseudoaffinity chromatography [3,26]. To this day, the vast body of work on thermoresponsive chromatography pertains to the modification and subsequent use of small pored inorganic (glass, silica) or hydrophobic (polystyrene based) chromatography supports in analytical HPLC separations of small biomolecules (especially steroids).…”

Section: Introductionmentioning

confidence: 99%

Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement

Müller

Cao

Ewert

et al. 2013

Journal of Chromatography A

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“…1), ionic [12,13], and affinity chromatography [14]. These temperature-responsive stationary phases may be useful in method development as an extra tool to optimize the selectivity by adjusting the temperature rather than changing the mobile phase composition.…”

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confidence: 99%

Temperature-responsive polymers for liquid-phase separations

Kanazawa¹

2004

Analytical and Bioanalytical Chemistry

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The needs of biotechnology for high-resolution purification and analytical technology have spurred new methods in chromatographic systems, in both terms of both columns and equipment.Stimuli-responsive polymers, which change their structure and physicochemical properties in response to external signals, comprise new materials with interesting applications in biomaterial science and technology. There are many synthetic polymers with molecular conformations sensitive to environmental factors, such as the pH [1], electric fields [2], and temperature [3]. Temperature seems to be one of the most attractive tools in such stimuli. Such polymers have been widely utilized for drug delivery systems [4], cell culture substrates [5], and bioconjugates [6]. However, there have been few reports concerning the use of these stimuli-responsive polymers in chromatographic separations.Poly N-isopropyl acrylamide (PNIPAAm) has the sharpest phase transition of the class of thermosensitive N-alkylacrylamide polymers. PNIPAAm exhibits thermally reversible soluble-insoluble changes in aqueous solution in response to temperature changes across a lower critical solution temperature (LCST) at 32°C [7]. The polymer chains show an expanded conformation in water below the LCST due to strong hydration and changes to compact forms above the LCST by dehydration. PNIPAAm-grafted surfaces show temperature-responsive hydrophilic-hydrophobic surface property alterations.By using these features, PNIPAAm and related polymers have been used to generate temperature-sensitive stationary phases for size exclusion [8], hydrophobic [9, 10, 11] (Fig. 1), ionic [12,13], and affinity chromatography [14]. These temperature-responsive stationary phases may be useful in method development as an extra tool to optimize the selectivity by adjusting the temperature rather than changing the mobile phase composition.The phase transition of PNIPAAm is due to the stability of the hydrophobic groups in the polymer chain in aqueous media. The LCST value for PNIPAAm has been regulated by the amount of hydrophobic comonomer, n-butyl methacrylate (BMA) [9]. LCSTs shift from 32°C to 20°C with increase in the mole fraction of BMA over a range of 1-5 mol% in the copolymer. With increasing BMA content, both the retention time and the affinity of the solutes were increased, even at a lower column temperature. Therefore, when separation at lower temperatures is necessary because of the stability of the proteins, use of the poly(NIPAAm-co-BMA) modified stationary phases is preferred. These hydrophobized stationary phases were also applicable for the separation of low molecular proteins, such as ribonuclease and chymotrypsinogen. Recently, the separation of basic bioactive peptides and other ionic compounds was achieved by utilizing copolymers of NIPAAm-introduced ion-exchange groups as a stationary phase [12]. PNIPAAm copolymers containing anionic acrylic acid (AAc) or cationic dimethylaminopropylacrylamide (DMAPPAm) produced alterable properties of the stationary phase with both th...

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Size exclusion behavior of hydroxypropylcellulose beads with temperature-dependent porosity

Cited by 38 publications

References 15 publications

Thermally responsive chromatographic materials using functional polymers

Thermally responsive chromatographic materials using functional polymers

Integrated system for temperature-controlled fast protein liquid chromatography comprising improved copolymer modified beaded agarose adsorbents and a travelling cooling zone reactor arrangement

Temperature-responsive polymers for liquid-phase separations

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