2020
DOI: 10.1016/j.colsurfb.2020.111053
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Size Measurement of Extracellular Vesicles and Synthetic Liposomes: The Impact of the Hydration Shell and the Protein Corona

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Cited by 78 publications
(61 citation statements)
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“…We therefore hypothesize that the structures surrounding the EVs are most likely a mixture of proteins and nucleic acids. In line with this, the group of Varga et al [30] has recently shown that EVs in vivo are also surrounded by a variety of different proteins that are not integrated in their own membrane. Furthermore, Jeppesen et al [26] were able to separate a protein fraction from a pure vesicle fraction and they demonstrated that different EV isolation methods impact the EV-miRNA composition.…”
Section: Ev Phenotypementioning
confidence: 71%
“…We therefore hypothesize that the structures surrounding the EVs are most likely a mixture of proteins and nucleic acids. In line with this, the group of Varga et al [30] has recently shown that EVs in vivo are also surrounded by a variety of different proteins that are not integrated in their own membrane. Furthermore, Jeppesen et al [26] were able to separate a protein fraction from a pure vesicle fraction and they demonstrated that different EV isolation methods impact the EV-miRNA composition.…”
Section: Ev Phenotypementioning
confidence: 71%
“…Due to their in vivo origin, EVs also can contain adsorbed proteins and protein coronas (Rosa-Fernandes et al, 2017 ; Karasu et al, 2018 ; Xia et al, 2019 ). One of the frequently used EVs are red blood cell derived EVs (REVs), which offer a reproducible system with high particle concentration (Szigyártó et al, 2018 ; Kitka et al, 2019 ; Deák et al, 2020 ; Varga et al, 2020 ).…”
Section: Introductionmentioning
confidence: 99%
“…Nano and quantum flow cytometers [25,29] are being developed to decrease this limit to 30 nm or smaller and typical sample volumes and number concentrations have not yet been established for these instruments. need standard size limit dilution error [24] Next Gen RPS 60 10 mL 10 6 to 10 10 need standard [18,19] FCM 80 100 mL 10 5 to 10 7 need standard [17] Quantum The virus counter is a specialized flow cytometer, designed to detect intact virus particles using a hydrodynamically focused nanostream and two fluorescence channels, one for nucleic acid detection and the other for capsid protein detection. No internal calibrant bead is required because the detected sample volume is determined by the instrument in real time.…”
Section: Submicrometer Particle Countingmentioning
confidence: 99%