Abstract. 3D bioprinter is an emerging interdisciplinary technology, which offers a new plan not only for the functional materials, ( droplets, drug delivery, organ regeneration, tissue engineering), but also for the new artificial vessel called 3D vessel bioprinter. In this paper, we developed a theoretical proposal to analyze the biosynsphere mechanical propertie under the multilayer fluid based on the 3D bioprinter, further analyze the broken mechanism to verify the theoretical. Our investigation shows that the outer fluid and inner fluid have difference effect on droplet, with the increase pressure of outer fluid, the force of droplet becomes greater, which revealed that the better pressure plays an important role in protecting the droplet.