Sizes and ligands tuned gold nanocluster acting as a new type of monoamine oxidase B inhibitor

Wu, Xin; Xu, Heng; Luo, Fazeng; Wang, Jinhua; Zhao, Li; Zhou, Xia; Yang, Ying; Cai, Huaihong; Sun, Ping‐Hua; Zhou, Haibo

doi:10.1016/j.bios.2021.113377

Cited by 12 publications

(10 citation statements)

References 62 publications

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“…Recently, AuNCs have been extensively demonstrated as nanoantibiotics for treating bacterial infections because of their unique chemical and physical properties. − Significant achievements have been made in the design of AuNCs with different functional ligands such as amino acids, peptides, enzymes, and antibiotics for antibacterial applications. − For instance, peptide-conjugated AuNCs loaded with vancomycin showed higher antimicrobial activity compared to vancomycin alone against both Gram-positive Staphylococcus aureus and Gram-negative E. coli .…”

Section: Introductionmentioning

confidence: 99%

Unveiling the Antibacterial Mechanism of Gold Nanoclusters via In Situ Transmission Electron Microscopy

Kuo

Tan

Hsiao

et al. 2021

ACS Sustainable Chem. Eng.

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Metal nanoclusters (NCs) with unique chemical and physical properties have been extensively demonstrated to be emerging nanoantibiotics for fighting bacterial infections. Understanding the antibacterial mechanisms of metal nanoclusters is important for evaluating their clinical applications as nanoantibiotics. To understand the antibacterial mechanism, gold nanoclusters (AuNCs) were applied as an antibacterial agent for real-time observations of their interactions with bacteria by in situ transmission electron microscopy (TEM). In this work, a surface ligand of glutathione-conjugated (GSH)-AuNCs was prepared via a simple hydrothermal method. Optical and structural characterizations validated the successful preparation of GSH-AuNCs. Bacterial growth curves of Acetobacter aceti revealed that the antibacterial activity of GSH-AuNCs increased with the weight concentration. The antibacterial activity of GSH-AuNCs was confirmed by the intracellular reactive oxygen species (ROS) generation induced by GSH-AuNCs in A. aceti. Furthermore, real-time observations of interactions between GSH-AuNCs and A. aceti were made using in situ liquid cell TEM. Based on the results of real-time observations, GSH-AuNCs first attached onto the bacterial membranes of A. aceti by physical adsorption and then penetrated into A. aceti by internalization. Eventually, the production of intracellular ROS induced by GSH-AuNCs caused destruction of the bacterial membranes, which led to the death of A. aceti. After the bacterial membranes had been destroyed, A. aceti eventually died.

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Section: Introductionmentioning

confidence: 99%

Unveiling the Antibacterial Mechanism of Gold Nanoclusters via In Situ Transmission Electron Microscopy

Kuo

Tan

Hsiao

et al. 2021

ACS Sustainable Chem. Eng.

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“…MAO-B, a metabolic enzyme for accounting for 80% of total MAO in the brain ( Ramsay, 2016 ) while only a little existed in peripheral platelets and lymphocytes ( Naoi et al, 2016 ), is considered to be expressed in neurons and glial cells. MAO-B is associated with neuronal apoptosis, cellular excitotoxicity and oxidative stress in neuropsychiatric diseases such as Parkinson’s disease (PD), Alzheimer’s disease (AD) and depression ( Tong et al, 2017 ; Park et al, 2019 ; Wu X. et al, 2021 ). It has been recently reported that ROS produced by MAO-B caused mitochondrial dysfunction and NF-κB activation, leading to NLRP3 inflammasome activation and IL-1β overexpression ( Sanchez-Rodriguez et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury

Zou

Gao

et al. 2022

Front. Pharmacol.

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Microglia are the resident macrophages in the brain, which play a critical role in post-stroke neuroinflammation. Accordingly, targeting neuroinflammation could be a promising strategy to improve ischemic stroke outcomes. Ethyl ferulate (EF) has been confirmed to possess anti-inflammatory properties in several disease models, including acute lung injury, retinal damage and diabetes-associated renal injury. However, the effects of EF on microglial activation and the resolution of post-stroke neuroinflammation remains unknown. Here, we found that EF suppressed pro-inflammatory response triggered by lipopolysaccharide (LPS) stimulation in primary microglia and BV2 cell lines, as well as post-stroke neuroinflammation in an in vivo transient middle cerebral artery occlusion (tMCAO) stroke model in C57BL/6 mice, consequently ameliorating ischemic brain injury. Furthermore, EF could directly bind and inhibit the activity of monoamine oxidase B (MAO-B) to reduce pro-inflammatory response. Taken together, our study identified a MAO-B inhibitor, Ethyl ferulate, as an active compound with promising potentials for suppressing post-stroke neuroinflammation.

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“…[147] Recently, metal NCs were reported to act as monoamine oxidase B inhibitors (MAOI), which paved the way for creating a more effective drug for Alzheimer's disease. [142] The Au NCs and enzymatic substrates exhibit competitive relationships. When Au NCs bind with MAO active sites like a plug, substrates cannot access the active sites; therefore, no enzymatic reaction can occur.…”

Section: Biocatalystsmentioning

confidence: 99%

Multiscale Bioresponses of Metal Nanoclusters

Shi,

Wu,

et al. 2024

Advanced Materials

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Metal nanoclusters (NCs) are well‐recognized novel nano‐agents that hold great promise for applications in nanomedicine because of their ultrafine size, low toxicity, and high renal clearance. As foreign substances, however, an in‐depth understanding of the bioresponses to metal NCs is necessary but is still far from being realized. Herein, this review is deployed to summarize the biofates of metal NCs at various biological levels, emphasizing their multiscale bioresponses at the molecular, cellular, and organismal levels. In the parts‐to‐whole schema, the interactions between biomolecules and metal NCs are discussed, presenting typical protein‐dictated nano–bio interfaces, hierarchical structures, and in vivo trajectories. Then, the accumulation, internalization, and metabolic evolution of metal NCs in the cellular environment and as‐imparted theranostic functionalization are demonstrated. The organismal metabolism and transportation processes of the metal NCs are subsequently distilled. Finally, this review ends with the conclusions and perspectives on the outstanding issues of metal NC‐mediated bioresponses in the near future. This review is expected to provide inspiration for tailoring the customization of metal NC‐based nano‐agents to meet practical requirements in different sectors of nanomedicine.

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Sizes and ligands tuned gold nanocluster acting as a new type of monoamine oxidase B inhibitor

Cited by 12 publications

References 62 publications

Unveiling the Antibacterial Mechanism of Gold Nanoclusters via In Situ Transmission Electron Microscopy

Unveiling the Antibacterial Mechanism of Gold Nanoclusters via In Situ Transmission Electron Microscopy

A monoamine oxidase B inhibitor ethyl ferulate suppresses microglia-mediated neuroinflammation and alleviates ischemic brain injury

Multiscale Bioresponses of Metal Nanoclusters

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