Sizes of water-soluble luminescent quantum dots measured by fluorescence correlation spectroscopy

Zhang, Pudun; Li, Liansheng; Dong, Chaoqing; Hu, Qian; Ren, Jicun

doi:10.1016/j.aca.2005.05.034

Cited by 57 publications

(51 citation statements)

References 30 publications

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“…Other techniques for size measurement include dynamic light scattering (Hoshino et al 2004;Pons et al 2006a;Liu et al 2007b;Yu et al 2007;, gel electrophoresis (Kimura et al 2002;Bücking & Nann 2006;Hanauer et al 2007;Park & Hamad-Schifferli 2008), size exclusion chromatography in both organic solvents (Steigerwald 1988;Wilcoxon et al 2000;Al-Somali et al 2004;Krueger et al 2005;Wang et al 2006) and aqueous phase (Siebrands et al 1993;Fischer et al 1994;Pinaud et al 2004;Carion et al 2007;Sperling et al 2007;Yu et al 2007;Smith & Nie 2008), analytical ultracentrifugation (Calabretta et al 2005;Jamison et al 2008;Lees et al 2008) or magnetic sedimentation (Berret et al 2007) or other techniques often based on particle diffusion, such as fluorescence correlation spectroscopy Liedl et al 2005;Zhang et al 2005), single-particle tracking (Lessard et al 2007;McHale et al 2007; or thermophoresis . A number of methods are limited to certain material classes as fluorescence particles, may be not compatible with them or their application may be hindered by the limited colloidal stability of the particles.…”

Section: (E) Characterizationmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

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Section: (E) Characterizationmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,409

1,092

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“…For this work, nanoparticle size was characterized by the following methods: transmission electron microscopy (TEM), [15][16][17] gel electrophoresis, 9,18-24 size exclusion chromatography (SEC), 7,8,21,[25][26][27][28][29][30][31][32][33][34][35] fluorescencecorrelationspectroscopy(FCS), 11,[36][37][38][39][40][41][42][43][44] and thermophoresis. [45][46][47] These and other relevant methods not used in this study are described in more detail in the Supporting Information (SI).…”

Section: Introductionmentioning

confidence: 99%

Size Determination of (Bio)conjugated Water-Soluble Colloidal Nanoparticles: A Comparison of Different Techniques

et al. 2007

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The size of inorganic colloidal nanoparticles coated with organic layers of different thickness has been measured with different techniques, including transmission electron microscopy, gel electrophoresis, size exclusion chromatography, fluorescence correlation spectroscopy, and thermophoresis. The results are critically compared, and the advantages and disadvantages of the respective methods are discussed.

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“…Primers were synthesised from Sun Biotech company, and their sequences were presented in Table 1. All other reagents, including molecular weight markers DL2000 (2000, 1000, 750, 500, 250 and NaHTe solution in the presence of mercaptopropionic acid as a stabiliser according to the approach described by Li et al [42] and Zhang [43]. Emission spectra were recorded using a Varian Cary spectrometer, and absorption spectra were obtained by a Lambda 20 UV-visible spectrophotometer (PerkinElmer).…”

Section: Methodsmentioning

confidence: 99%

Quantum dots trigger hot-start effects for pfu-based polymerase chain reaction

Sang

Yang

Wang

et al. 2013

Journal of Experimental Nanoscience

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Hot-start (HS) effects were investigated in pfu-based polymerase chain reaction (PCR), when water-soluble CdTe quantum dots (QDs) were introduced in the PCR system. The HS effects were demonstrated by the higher amplicon yields and excellent suppression of non-specific amplification after pre-incubation of PCR mix with QDs between 35 C and 56 C. DNA targets were well amplified even after PCR mixture was pre-incubated 1 h at 50 C. Importantly, the effects of QDs nanoparticles could be reversed by increasing the pfu polymerase concentration, suggesting that there was an interaction between QDs and pfu DNA polymerase. Moreover, control experiment indicated that HS effect is not primarily due to the reduced pfu polymerase concentration resulted from the above interaction. Fluorescence correlation spectroscopy (FCS), a single molecule detection method, was used to investigate the possible mechanism of HS PCR with QDs. Preliminary FCS results suggested that CdTe QDs may directly interact with pfu DNA polymerase, rather than other components in the PCR system. Furthermore, results demonstrated that the interaction between QDs and pfu resulted in a reduction in pfu polymerase concentration. This study provided a good start to investigate potential implications of QDs in other key molecular biology techniques.

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Sizes of water-soluble luminescent quantum dots measured by fluorescence correlation spectroscopy

Cited by 57 publications

References 30 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Size Determination of (Bio)conjugated Water-Soluble Colloidal Nanoparticles: A Comparison of Different Techniques

Quantum dots trigger hot-start effects for pfu-based polymerase chain reaction

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