SKAP2 is downregulated in the villous tissues of patients with missed abortion and regulates growth and migration in trophoblasts through the WAVE2-ARP2/3 signaling pathway

Jin, Xuejing; Chen, Yijie; Sheng, Zhumei; Wang, Xue; Zhang, Zhifen; Huang, Jikun; Zhou, Jie; Lin, Feiyun

doi:10.1016/j.placenta.2022.08.061

Cited by 8 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ESR1 and MXD1 showed a low expression level, while SKAP2 and CLEC4D were significantly downregulated (p ≤ 0.05) in the mRNA expression level measured by RT-qPCR in our study. SKAP2 was downregulated in the villous tissues of patients with failed pregnancies [48], while MXD1 was associated with early and late gestation in patients with severe preeclampsia [49]. Contrastingly, GATM was identified as upregulated in the SFH group in our study, but no significant difference was observed in the mRNA expression level measured by RT-qPCR (p ≥ 0.05).…”

Section: Discussioncontrasting

confidence: 69%

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

Banerjee

Diniz

Hollingsworth

et al. 2023

Genes

View full text Add to dashboard Cite

Reproductive failure is a major contributor to inefficiency within the cow-calf industry. Particularly problematic is the inability to diagnose heifer reproductive issues prior to pregnancy diagnosis following their first breeding season. Therefore, we hypothesized that gene expression from the peripheral white blood cells at weaning could predict the future reproductive potential of beef heifers. To investigate this, the gene expression was measured using RNA-Seq in Angus–Simmental crossbred heifers sampled at weaning and retrospectively classified as fertile (FH, n = 8) or subfertile (SFH, n = 7) after pregnancy diagnosis. We identified 92 differentially expressed genes between the groups. Network co-expression analysis identified 14 and 52 hub targets. ENSBTAG00000052659, OLR1, TFF2, and NAIP were exclusive hubs to the FH group, while 42 hubs were exclusive to the SFH group. The differential connectivity between the networks of each group revealed a gain in connectivity due to the rewiring of major regulators in the SFH group. The exclusive hub targets from FH were over-represented for the CXCR chemokine receptor pathway and inflammasome complex, while for the SFH, they were over-represented for immune response and cytokine production pathways. These multiple interactions revealed novel targets and pathways predicting reproductive potential at an early stage of heifer development.

show abstract

Section: Discussioncontrasting

confidence: 69%

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

Banerjee

Diniz

Hollingsworth

et al. 2023

Genes

View full text Add to dashboard Cite

show abstract

“…4 a and b), among which, PDGFD , ADCY1 , PIK3CA , SKAP1 , LPAR3 , KITLG , and SIPA1L2 were enriched in the Rap1 signaling pathway, TP53 , ERBB4 , TNR , PIK3CA , TLR2 , JAK1 , LPAR3 , KITLG , and PDGFD in the PI3K-Akt signaling pathway, TSHR , PIK3CA , ADCY1 , and PTGER3 in the lipolysis regulation of part of the adipocyte pathway and PLCG2 , TP53 , PIK3CA , and DSC1 in the thyroid hormone signaling pathway. Moreover, the ABCD4 , SKAP2 , and SLITRK2 genes were also selected in the warm-temperature group with ABCD4 having a role in the number of vertebrae, which is an important economic trait in sheep production and increases body weight [ 39 ], SKAP2 being involved in the immune system [ 40 ] and SLITRK2 in the nervous system. Overall, we have genetic evidence for traits such as reproduction, adipose regulation, and metabolism that could be under selection during adaptation to warm areas.…”

Section: Resultsmentioning

confidence: 99%

Whole-genome resequencing of Chinese indigenous sheep provides insight into the genetic basis underlying climate adaptation

Jin,

Wang,

Liu

et al. 2024

Genet Sel Evol

View full text Add to dashboard Cite

Background Chinese indigenous sheep are valuable resources with unique features and characteristics. They are distributed across regions with different climates in mainland China; however, few reports have analyzed the environmental adaptability of sheep based on their genome. We examined the variants and signatures of selection involved in adaptation to extreme humidity, altitude, and temperature conditions in 173 sheep genomes from 41 phenotypically and geographically representative Chinese indigenous sheep breeds to characterize the genetic basis underlying environmental adaptation in these populations. Results Based on the analysis of population structure, we inferred that Chinese indigenous sheep are divided into four groups: Kazakh (KAZ), Mongolian (MON), Tibetan (TIB), and Yunnan (YUN). We also detected a set of candidate genes that are relevant to adaptation to extreme environmental conditions, such as drought-prone regions (TBXT, TG, and HOXA1), high-altitude regions (DYSF, EPAS1, JAZF1, PDGFD, and NF1) and warm-temperature regions (TSHR, ABCD4, and TEX11). Among all these candidate genes, eight ABCD4, CNTN4, DOCK10, LOC105608545, LOC121816479, SEM3A, SVIL, and TSHR overlap between extreme environmental conditions. The TSHR gene shows a strong signature for positive selection in the warm-temperature group and harbors a single nucleotide polymorphism (SNP) missense mutation located between positions 90,600,001 and 90,650,001 on chromosome 7, which leads to a change in the protein structure of TSHR and influences its stability. Conclusions Analysis of the signatures of selection uncovered genes that are likely related to environmental adaptation and a SNP missense mutation in the TSHR gene that affects the protein structure and stability. It also provides information on the evolution of the phylogeographic structure of Chinese indigenous sheep populations. These results provide important genetic resources for future breeding studies and new perspectives on how animals can adapt to climate change.

show abstract

“…Xuejing Jin et al found that SKAP2 indirectly affected F-actin reassembly through activation of the WAVE2-ARP2/3 pathway, thereby increasing trophoblast growth and migration. 25 It has also been found that HTR8/SVneo cell invasion could be increased by inducing actin polymerization. 26 In addition, during the early stages of human placental development, activation of the FAK signaling pathway enhanced the proliferation, migration, and invasion of human trophoblast cells.…”

Section: Discussionmentioning

confidence: 99%

“…Xuejing Jin et al. found that SKAP2 indirectly affected F‐actin reassembly through activation of the WAVE2‐ARP2/3 pathway, thereby increasing trophoblast growth and migration 25 . It has also been found that HTR8/SVneo cell invasion could be increased by inducing actin polymerization 26 .…”

Section: Discussionmentioning

confidence: 99%

ELF1/PRR11/ARP2/3 promoted trophoblast cells proliferation and motility in early pregnancy

Zhu,

Dou,

Song

et al. 2023

American J Rep Immunol

View full text Add to dashboard Cite

Background/objectiveEarly pregnancy loss (EPL) is a common adverse pregnancy outcome with an incidence of approximately 10–30%. There are many factors that cause EPL, among which the lack of proliferation and invasive properties of trophoblast cells can lead to embryonic development. Therefore, in this study, the molecular biology of trophoblast cells was investigated.MethodsPlacental villous tissues from EPL patients were collected to explore ELF1 and PRR11 gene expression. The proliferation and migration of trophoblast cells were assessed by MTT, crystalline violet staining, and traswell assays, respectively. Western blotting and RT‐qPCR were performed to investigate the relationship between ELF1, PRR11, and ARP2/3. F‐actin polymerization and FAK activation were evaluated by immunofluorescence and western blotting. Ultimately, ELF1/PRR11/ARP2/3 expression was verified in the EPL mice modelResultsELF1 and PRR11 were lowly expressed in placental villous tissues from EPL. The overexpression of ELF1 and PRR11 promoted proliferation and migration of trophoblast cells. Moreover, while ELF1 bound to the PRR11 promoter and promoted transcriptional activation. Finally, ELF1/PRR11/ARP2/3 showed low expression in the placental tissue of EPL mice.ConclusionOur study suggested that PRR11 promoted the motility of trophoblast cells by binding to the ARP2/3 complex to promote F‐actin polymerization and FAK activation. In addition, ELF1 bound to the initiation site of PRR11 to promote its transcription. ELF1/PRR11/ARP2/3 may play an important role in EPL.

show abstract

SKAP2 is downregulated in the villous tissues of patients with missed abortion and regulates growth and migration in trophoblasts through the WAVE2-ARP2/3 signaling pathway

Cited by 8 publications

References 25 publications

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

mRNA Signatures in Peripheral White Blood Cells Predict Reproductive Potential in Beef Heifers at Weaning

Whole-genome resequencing of Chinese indigenous sheep provides insight into the genetic basis underlying climate adaptation

ELF1/PRR11/ARP2/3 promoted trophoblast cells proliferation and motility in early pregnancy

Contact Info

Product

Resources

About