Skeletal Muscle‐Derived Stem/Progenitor Cells: A Potential Strategy for the Treatment of Acute Kidney Injury

Pavydė, Eglė; Mačiulaitis, Romaldas; Mauricas, Mykolas; Sudzius, Gintaras; Didžiokienė, Ernesta Ivanauskaitė; Laurinavičius, Arvydas; Sutkevičienė, Neringa; Stankevičius, Edgaras; Mačiulaitis, Justinas; Ūsas, Arvydas

doi:10.1155/2016/9618480

Cited by 17 publications

(19 citation statements)

References 50 publications

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“…To track and locate the transplanted hAD-MSCs in the ovarian tissues, the cells were pre-labeled with the PKH26 Red Fluorescent Cell Linker Kits (Sigma-Aldrich, St. Louis, MO, USA) [ 31 , 32 ]. The PKH26-labeled hAD-MSCs were transplanted into rats via the tail vein.…”

Section: Methodsmentioning

confidence: 99%

Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats

Feng

Wei

et al. 2017

Stem Cell Res Ther

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BackgroundHuman amnion-derived mesenchymal stem cells (hAD-MSCs) have the features of mesenchymal stem cells (MSCs). Low-intensity pulsed ultrasound (LIPUS) can promote the expression of various growth factors and anti-inflammatory molecules that are necessary to keep the follicle growing and to reduce granulosa cell (GC) apoptosis in the ovary. This study aims to explore the effects of LIPUS-pretreated hAD-MSC transplantation on chemotherapy-induced primary ovarian insufficiency (POI) in rats.MethodsThe animals were divided into control, POI, hAD-MSC treatment, and LIPUS-pretreated hAD-MSC treatment groups. POI rat models were established by intraperitoneal injection of cyclophosphamide (CTX). The hAD-MSCs isolated from the amnion were exposed to LIPUS or sham irradiation for 5 consecutive days and injected into the tail vein of POI rats. Expression and secretion of growth factors promoted by LIPUS in hAD-MSCs were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) in vitro. Estrous cycle, serum sex hormone levels, follicle counts, ovarian pathological changes, GC apoptosis, Bcl2 and Bax expression, and pro-inflammatory cytokine levels in ovaries were examined.ResultsPrimary hAD-MSCs were successfully isolated from the amnion. LIPUS promoted the expression and secretion of growth factors in hAD-MSCs in vitro. Both hAD-MSC and LIPUS-pretreated hAD-MSC transplantation increased the body and reproductive organ weights, improved ovarian function, and reduced reproductive organ injuries in POI rats. Transplantation of hAD-MSCs increased the Bcl-2/Bax ratio and reduced GC apoptosis and ovarian inflammation induced by chemotherapy in ovaries. These effects could be improved by pretreatment with LIPUS on hAD-MSCs.ConclusionBoth hAD-MSC transplantation and LIPUS-pretreated hAD-MSC transplantation can repair ovarian injury and improve ovarian function in rats with chemotherapy-induced POI. LIPUS-pretreated hAD-MSC transplantation is more advantageous for reducing inflammation, improving the local microenvironment, and inhibiting GC apoptosis induced by chemotherapy in ovarian tissue of POI rats.

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Section: Methodsmentioning

confidence: 99%

Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats

Feng

Wei

et al. 2017

Stem Cell Res Ther

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“…These cells are multipotent cells, demonstrating high self-renewal and long-term proliferation capacities. These cells are able to promote endogenous tissue repair by secreting trophic factors [26][27][28]. Therefore, they are a valuable source for regenerative medicine and tissue engineering applications.…”

Section: Resultsmentioning

confidence: 99%

Biocompatibility Evaluation and Enhancement of Elastomeric Coatings Made Using Table-Top Optical 3D Printer

et al. 2020

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In this experimental report, the biocompatibility of elastomeric scaffold structures made via stereolithography employing table-top 3D printer Ember (Autodesk) and commercial resin FormLabs Flexible (FormLabs) was studied. The samples were manufactured using the standard printing and development protocol, which is known to inherit cytotoxicity due to remaining non-polymerized monomers, despite the polymerized material being fully biocompatible. Additional steps were taken to remedy this problem: the fabricated structures were soaked in isopropanol and methanol under different conditions (temperature and duration) to leach out the non-polymerized monomers. In addition, disc-shaped 3D-printed structures were UV exposed to assure maximum polymerization degree of the material. Post-processed structures were seeded with myogenic stem cells and the number of live cells was evaluated as an indicator for the material biocompatibility. The straightforward post-processing protocol enhanced the biocompatibility of the surfaces by seven times after seven days soaking in isopropanol and methanol and was comparable to control (glass and polystyrene) samples. This proposes the approach as a novel and simple method to be widely applicable for dramatic cytotoxicity reduction of optically 3D printed micro/nano-scaffolds for a wide range of biomedical studies and applications.

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“…We dissected the femoral artery and vein from surrounding tissues to induce complete ischemia by a clamp; however, most of study only ties the hind limb. The latter case could give stress on the skeletal muscle and might stimulate the interaction between muscle and the kidney [16,17].…”

Section: Discussionmentioning

confidence: 99%

Remote Ischemic Preconditioning of the Femoral Artery and Vein does not Protect against Renal Ischemia/Reperfusion-Induced Injury in Anesthetized Mice

Zhang¹,

Nakano²,

Nishiyama³

2016

JURNOA

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Journal of Urology and Nephrology Open Access Open Access Short Communication are not consistent; some reported benefits [3-5], while others reported neutral effects [6,7]. Therefore, the aim of the present study is to develop an experimental RIPC model against Renal Ischemia/Reperfusion (I/R)-induced AKI for the further evaluation of complexity observed in the clinical studies. Methods and Materials Male C57BL/6 mice at 5 weeks of age were purchased from CLEA (Tokyo, Japan). The mice were uninephrectomized 10 days prior to I/R and RIPC. We examined seven different RIPC protocols to determine whether RIPC induces renal protection against renal I/R injury in either pentobarbital (Figure 1 and 2) or isoflurane (Figure 3) anesthetized mice. All experimental procedures were performed according to the guidelines for the care and use of animals established by Kagawa University. RIPC was conducted by occluding the femoral artery and vein in the right hind limb by noninvasive vascular clamp, except for one protocol using low temperature-induced ischemia. We observed the right hind limb turn white during ischemia, and return to normal color after reperfusion. Renal I/R were performed in the left kidney of animals that had received a right uninephrectomized 10 days prior to I/R, under body temperature-controlled condition. Blood Urea Nitrogen (BUN) level was assessed as previously described [2]. Plasma BUN levels were measured using commercially available assay kits (Urea nitrogen B test; Wako, Osaka, Japan). For the histology, kidney tissue was fixed with 4% par formaldehyde (pH = 7.4), embedding in paraffin and sectioned into 3 μm thick slides, and stained with hematoxylin/ eosin. We used an optical microscope (BX-51/DP-72; Olympus, Tokyo, Japan) to observe renal histological changes. Semi-quantitatively analyzed HE scores were given as average values of loss of the brush border, tubular dilatation, cast formation, and congestion/ hemorrhage.

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Skeletal Muscle‐Derived Stem/Progenitor Cells: A Potential Strategy for the Treatment of Acute Kidney Injury

Cited by 17 publications

References 50 publications

Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats

Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats

Biocompatibility Evaluation and Enhancement of Elastomeric Coatings Made Using Table-Top Optical 3D Printer

Remote Ischemic Preconditioning of the Femoral Artery and Vein does not Protect against Renal Ischemia/Reperfusion-Induced Injury in Anesthetized Mice

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