Skeletal muscle dispersion (400‐1000 nm) and kinetics at optical clearing

Oliveira, Luís; Carvalho, M. I.; Nogueira, E.; Tuchin, Valery V.

doi:10.1002/jbio.201700094

Cited by 33 publications

(25 citation statements)

References 42 publications

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“…Strongly bound water, which is approximately 44% of the total water in the skin (0‐200 μm), can also take part in this flux, but at lower mobility. These results are in good agreement with the results presented in and are slightly lower that the result obtained for muscle tissues . This difference can be explained by the skin heterogeneity and different water bounding properties of epidermis and dermis in comparison with other tissues.…”

Section: Resultssupporting

confidence: 91%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

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Confocal Raman microscopy has been used to measure depth‐dependent profiles of porcine skin ex vivo in the high wavenumber region after application of molecular optical clearing agents (OCAs). Glycerol (70%) and iohexol (100% Omnipaque [300]) water solutions were used as OCAs and topically applied to porcine ear skin for 30 and 60 minutes. Using Gaussian function–based deconvolution, the changes of hydrogen bound water molecule types have been microscopically analyzed down to the depth of 200 μm. Results show that both OCAs induced skin dehydration (reduction of total water), which is 51.3% for glycerol (60 minutes), 33.1% for glycerol (30 minutes), 8.3% for Omnipaque (60 minutes) and 4.4% for Omnipaque (30 minutes), on average for the 40 to 200 μm depths. Among the water types in the skin, the following reduction was observed in concentration of weakly bound (51.1%, 33.2%, 7.5% and 4.6%), strongly bound (50.4%, 33.0%, 7.9% and 3.4%), tightly bound (63.6%, 42.3%, 26.1% and 12.9%) and unbound (55.4%, 28.7%, 10.1% and 5.9%) water types on average for the 40 to 200 μm depths, post application of glycerol (60 minutes), glycerol (30 minutes), Omnipaque (60 minutes) and Omnipaque (30 minutes), respectively. As most concentrated in the skin, weakly and strongly bound water types are preferentially involved in the OCA‐induced water flux in the skin, and thus, are responsible for optical clearing efficiency.

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Section: Resultssupporting

confidence: 91%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

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“…Biological tissues are heterogeneous materials, since they contain cells and their organelles, protein fibers, DNA and lipids, which are surrounded by tissue fluids like the cytoplasm and the interstitial fluid . Such heterogeneous composition provides a global set of optical properties for the tissues.…”

Section: Introductionmentioning

confidence: 99%

Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Carneiro

Carvalho

Henrique

et al. 2019

Journal of Biophotonics

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The optical immersion clearing technique has been successfully applied through the last 30 years in the visible to near infrared spectral range, and has proven to be a promising method to promote the application of optical technologies in clinical practice. To investigate its potential in the ultraviolet range, collimated transmittance spectra from 200 to 1000 nm were measured from colorectal muscle samples under treatment with glycerol‐water solutions. The treatments created two new optical windows with transmittance efficiency peaks at 230 and 300 nm, with magnitude increasing with glycerol concentration in the treating solution. Such discovery opens the opportunity to develop clinical procedures to perform diagnosis or treatments in the ultraviolet.

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“…However, the autofluorescence remains an issue because of myoglobin and NADH presence, the endogenously fluorescent molecules which limit skeletal muscle tissue imaging especially in ≈488 nm spectrum . Moreover, skeletal muscle tissue alone presents with high RI mismatch between muscle fluids (RI = 1.35, 72–80% of muscle weight) and muscle proteins (RI = 1.53, 20–28% of muscle weight) . Such discrepancies between skeletal and cardiac muscle results in different trends of TOC methods utilization.…”

Section: Application Of Toc For Particular Peripheral Organsmentioning

confidence: 99%

Advances in Ex Situ Tissue Optical Clearing

Matryba

Kaczmarek

Gołąb

2019

Laser & Photonics Reviews

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Examination of whole organs with subcellular resolution in health, disease, and during development is necessary to decipher their biological complexity. However, until recently, this has been virtually impossible due to the natural opacity of organ tissue. Recent progress in tissue optical clearing (TOC) has overcome this limitation by turning organs into transparent, light-permitting specimens. At least 20 original TOC methods have been developed in less than a decade, which were followed by hundreds of attempts that were aimed at their optimization and practical application. The majority of proof-of-concept studies have focused on the brain. However, it is apparent that TOC might be equally valuable when applied to peripheral organs or even the whole body. The progress in TOC for peripheral organs is delineated in an organ-by-organ fashion and whole-body clearing approaches are discussed. Additionally, physical and optical approaches for TOC affecting the optical properties of the samples and image quality are discussed to explore their advantages, limitations, and future possibilities.

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Skeletal muscle dispersion (400‐1000 nm) and kinetics at optical clearing

Cited by 33 publications

References 42 publications

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Advances in Ex Situ Tissue Optical Clearing

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