Skeletal myogenesis and<i>Myf5</i>activation

Francetic, Tanja; Li, Qiao

doi:10.4161/trns.2.3.15829

Cited by 78 publications

(82 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Myf5 is the first MRF to be expressed, which marks the commitment of progenitor cells into skeletal muscle lineage [2]. We found that similar to Pax3, Myf5 transcripts were also detected by day 4 of differentiation (Figure 4A).…”

Section: Resultsmentioning

confidence: 70%

Regulation of Myf5 Early Enhancer by Histone Acetyltransferase P300 during Stem Cell Differentiation

et al. 2012

Self Cite

View full text Add to dashboard Cite

Skeletal myogenesis is an intricate process coordinated temporally by multiple myogenic regulatory factors (MRF) including Myf5, which is the first MRF expressed and marks the commitment of skeletal muscle lineage. The expression of Myf5 gene during early embryogenesis is controlled by a set of enhancer elements, and requires the histone acetyltransferase (HAT) activity of transcriptional coactivator p300. However, it is unclear as to how different regulatory signals converge at enhancer elements to regulate early Myf5 gene expression, and if p300 is directly involved. We show here that p300 associates with the Myf5 early enhancer at the early stage of stem cell differentiation, and its HAT activity is important for the recruitment of β-catenin to this early enhancer. In addition, histone H3-K27 acetylation, but not H3-K9/14, is intimately connected to the p300 HAT activity. Thus, p300 is directly involved in the regulation of the Myf5 early enhancer, and is important for specific histone acetylation and transcription factor recruitment. This connection of p300 HAT activity with H3-K27 acetylation and β-catenin signalling during myogenic differentiation in vitro offers a molecular insight into the enhancer-elements participation observed in embryonic development. In addition, pluripotent stem cell differentiation is a valuable system to dissect the signal-dependent regulation of specific enhancer element during cell fate determinations.

show abstract

Section: Resultsmentioning

confidence: 70%

Regulation of Myf5 Early Enhancer by Histone Acetyltransferase P300 during Stem Cell Differentiation

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sequential expression of the myogenic regulatory factors such as Myf5, MyoD, and myogenin, is essential for spatiotemporal expression of muscle specific genes, which is controlled by progressive activation of complex cis -regulatory elements13.…”

mentioning

confidence: 99%

Stepwise acetyltransferase association and histone acetylation at the Myod1 locus during myogenic differentiation

Hamed

Khilji

Chen

et al. 2013

Sci Rep

Self Cite

View full text Add to dashboard Cite

While chromatin modifications can offer a useful readout for enhancer activities, it is less clear whether these modification marks are a cause or consequence of transcription factor occupancy and enhancer activation. We have examined in details the temporal events of acetyltransferase associations and histone acetylations at different regulatory regions of the Myod1 locus. Our studies demonstrate that the histone acetyltransferase (HAT) p300 is stepwise enriched at distinct Myod1 regulatory regions during myogenic differentiation. This enrichment of p300 is associated with increased histone acetylation in a discrete pattern. Inhibition of p300 HAT activity impedes myogenic differentiation, which is coupled with decreased histone acetylation at specific Myod1 regulatory regions. We show for the first time that p300 is directly involved in the early regulation of Myod1 enhancer, and provide molecular insights into how p300 HAT activity and histone acetylation are related to enhancer activation and, consequently, gene transcription.

show abstract

“…These transcription factors are essential for the determination and differentiation of skeletal muscle during embryogenesis (18, 45), and display the unique ability to convert non-muscle cell types to skeletal muscle (58). Myf5 is the first MRF to be expressed, at E8 in the mouse (42).…”

Section: Introductionmentioning

confidence: 99%

Pax3 synergizes with Gli2 and Zic1 in transactivating the Myf5 epaxial somite enhancer

Himeda

Barro

Emerson

2013

Developmental Biology

View full text Add to dashboard Cite

Both Glis, the downstream effectors of hedgehog signaling, and Zic transcription factors are required for Myf5 expression in the epaxial somite. Here we demonstrate a novel synergistic interaction between members of both families and Pax3, a paired-domain transcription factor that is essential for both myogenesis and neural crest development. We show that Pax3 synergizes with both Gli2 and Zic1 in transactivating the Myf5 epaxial somite (ES) enhancer in concert with the Myf5 promoter. This synergy is dependent on conserved functional domains of the proteins, as well as on a novel homeodomain motif in the Myf5 promoter and the essential Gli motif in the ES enhancer. Importantly, overexpression of Zic1 and Pax3 in the 10T1/2 mesodermal cell model results in enrichment of these factors at the endogenous Myf5 locus and induction of Myf5 expression. In our previous work, we showed that by enhancing nuclear translocation of Gli factors, Zics provide spatiotemporal patterning for Gli family members in the epaxial induction of Myf5 expression. Our current study indicates a complementary mechanism in which association with DNA-bound Pax3 strengthens the ability of both Zic1 and Gli2 to transactivate Myf5 in the epaxial somite.

show abstract

Skeletal myogenesis andMyf5activation

Cited by 78 publications

References 76 publications

Regulation of Myf5 Early Enhancer by Histone Acetyltransferase P300 during Stem Cell Differentiation

Regulation of Myf5 Early Enhancer by Histone Acetyltransferase P300 during Stem Cell Differentiation

Stepwise acetyltransferase association and histone acetylation at the Myod1 locus during myogenic differentiation

Pax3 synergizes with Gli2 and Zic1 in transactivating the Myf5 epaxial somite enhancer

Contact Info

Product

Resources

About