Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals

Akintoye, Sunday O.; Lam, T.; Shi, Songtao; Brahim, Jaime S.; Collins, Michael T.; Robey, Pamela Gehron

doi:10.1016/j.bone.2005.10.027

Cited by 322 publications

(349 citation statements)

References 39 publications

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“…MSCs have been also isolated from the mandibular bone (Akintoye et al, 2006). Similarly to iliac crest-derived MSCs, stem cells originated from the mandible are clonogenic and exhibit osteogenic potential both in vitro and in vivo.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Pagella

Neto

Lamghari

et al. 2015

eCM

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Stem cell-based mediated therapies represent very promising approaches for tissue regeneration and are already applied with success in clinics. These therapeutic approaches consist of the in vitro manipulation of stem cells and their consequent administration to patients as living and dynamic biological agents. Nevertheless, the deregulation of stem cells function might result in the generation of pathologies such as tumours or accelerated senescence. Moreover, different stem cells sources are needed for regeneration of specific tissues. It is thus fundamental to understand the mechanisms regulating the physiology of stem cells. Microfluidic technology can be used to mimic in vivo scenarios and allow the study of stem cell physiology at both single cell and whole stem cell niche levels. This review focuses on the potential sources of stem and progenitor cells for orofacial regeneration and the use of microfluidic technologies for the study of stem cells behaviour and stem cell niches, in the light of regenerative medicine. Keywords:Microfluidics, stem cells, stem cell niches, orofacial regeneration, tooth, innervation, trigeminal ganglia, regenerative medicine. IntroductionThe development of organs and tissues that belong to the orofacial complex proceeds through a series of inductive interactions between cells originated from the epithelium, mesoderm and cranial neural crest-derived mesenchyme (Mao et al., 2012;Mitsiadis and Graf, 2009;Mitsiadis and Papagerakis, 2011). Orofacial organs are highly diverse and exert fundamental and specific functions such as breathing, chewing, speech, smell, and sight (Mao et al., 2012). The physiological functions of these organs are compromised by traumatic injuries, congenital and infectious diseases, and cancer (Mao et al., 2012;Scheller et al., 2009). Furthermore, these pathologies are often accompanied by intensive pain and aesthetic deformities. Therefore, the treatment of compromised pathological orofacial tissues and organs should guarantee restoration of both functionality and aesthetics, which constitutes an enormous clinical challenge. Moreover, organ structure, function, aesthetics, and pain should be managed simultaneously during the regenerative care, a situation that is more complex than in other compartments of the body (Scheller et al., 2009).Biological regeneration is proving an increasingly attractive alternative and complement to traditional surgical techniques for prosthetic replacement of tissues and organs. Cell-based therapeutic approaches are already applied with success in clinics and consist of in vitro manipulation of stem cells and their consequent administration to patients as living and dynamic biological agents. Stem cells are characterised by their potential to self-replicate and their capacity to differentiate into a vast variety of cell types that form the diverse tissues. Therefore, stem cells guarantee tissue repair and regeneration throughout life. During the last decades, a plethora of adult stem cell populations have been isolate...

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Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Pagella

Neto

Lamghari

et al. 2015

eCM

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“…Despite their intra-osseous origin, topographical differences in MSCs recovered from different types of bones do exist (Akintoye et al, 2006). More recently, MSCs have been topographically linked to both pericytes (Caplan, 2008;Crisan et al, 2008;Meirelles et al, 2008;Caplan SM Churchman et al Molecular profile of MSCs and Correa, 2011) and bone-lining cells (Tormin et al, 2011;Rasini et al, 2013).…”

Section: Molecular Comparison Between Iliac Crest and Ria Fluid: Molementioning

confidence: 99%

Yield optimisation and molecular characterisation of uncultured CD271+ mesenchymal stem cells in the reamer irrigator aspirator waste bag

Churchman

Kouroupis²,

Boxall³

et al. 2013

eCM

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Bone reconstruction requires the use of autografts from patients' iliac crest (IC); for large-volume defects bone void fillers and autologous mesenchymal stem cells (MSCs) are often added. The Reamer/Irrigator/Aspirator (RIA) device provides the means of harvesting large amounts of autograft and additionally yields a waste bag containing MSCs, which is currently discarded. The aim of this study was to enumerate and characterise native MSCs from RIA waste bag and compare them to 'gold-standard' donormatched MSCs from IC bone marrow (BM). IC-BM from age matched trauma patients was used as control.In RIA waste bags the median MSC yield established using a colony-forming fibroblast assay was 314333 (range 5 x 10 4 -1.4 x 10 6 ), equivalent to approximately one litre of IC-BM aspirate. CD271+ cells were present at high levels in RIA waste bags, had MSC surface phenotype (CD90 + CD73 + CD105 + CD34 -CD61 -CD19 -CD31 -CD33 -) and expressed genes associated with multipotentiality, osteogenesis, adipogenesis and angiogenic support. RIA-CD271 + MSCs were transcriptionally similar to donormatched IC-CD271 + MSCs (76 % transcripts); with the majority of bone-related and Wnt pathway molecules being expressed at comparable levels. Lower-level expression of MCAM/CD146 and 5/13 hypoxia-related molecules was found in RIA-CD271 + MSCs, potentially reflecting their native residence in a more hypoxic environment of the endosteum and bone cortex.These data suggest that long bones contain very large numbers of MSCs, transcriptionally-similar to IC-BM MSCs; they can be procured by reaming using the RIA device and used, following concentration, as autologous and potentially allogeneic bone repair therapy.

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“…[6][7][8] An important issue with any tissue-engineered bone implant is matching the tissue engineering strategy to patient's and implant's site-specific bone regeneration rates to ensure enhanced integration and bone remodeling. 5,9 A number of studies have been conducted to tailor biomaterial matrix design to meet specific bone repair needs. In the case of silk biomaterials, significant control over bone morphology can be achieved by altering scaffold design features, for example, stiffness, pore size, interconnectivity between pores, degradability, and processing methods.…”

mentioning

confidence: 99%

Hypoxia and Amino Acid Supplementation Synergistically Promote the Osteogenesis of Human Mesenchymal Stem Cells on Silk Protein Scaffolds

Sengupta

Park

Patel

et al. 2010

Tissue Engineering Part A

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Tailoring tissue engineering strategies to match patient-and tissue-specific bone regeneration needs offers to improve clinical outcomes. As a step toward this goal, osteogenic outcomes and metabolic parameters were assessed when varying inputs into the bone formation process. Silk protein scaffolds seeded with human mesenchymal stem cells in osteogenic differentiation media were used to study in vitro osteogenesis under varied conditions of amino acid (lysine and proline) concentration and oxygen level. The cells were assessed to probe how the microenvironment impacted metabolic pathways and thus osteogenesis. The most favorable osteogenesis outcomes were found in the presence of low (5%) oxygen combined with high lysine and proline concentrations during in vitro cultivation. This same set of culture conditions also showed the highest glucose consumption, lactate synthesis, and certain amino acid consumption rates. On the basis of these results and known pathways, a holistic metabolic model was derived which shows that lysine and proline supplements as well as low (5%) oxygen levels regulate collagen matrix synthesis and thereby rates of osteogenesis. This study establishes early steps toward a foundation for patient-and tissue-specific matches between metabolism, repair site, and tissue engineering approaches toward optimized bone regeneration.

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Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals

Cited by 322 publications

References 39 publications

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Investigation of orofacial stem cell niches and their innervation through microfluidic devices

Yield optimisation and molecular characterisation of uncultured CD271+ mesenchymal stem cells in the reamer irrigator aspirator waste bag

Hypoxia and Amino Acid Supplementation Synergistically Promote the Osteogenesis of Human Mesenchymal Stem Cells on Silk Protein Scaffolds

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