According to the kinematics analysis of the human body, a set of exoskeleton mechanical structures is designed to imitate the physiological structure and movement characteristics of the lower limbs of humans. To study the biomechanical characteristics of the exoskeleton in two different phases during walking and to provide a research basis for the design and optimization of the exoskeleton. Doctors and engineers are actively committed to the basic understanding and improvement of the tissue characteristics, structure, and function of the human musculoskeletal system. Firstly, according to the gait analysis of the lower limbs, the exoskeleton mechanical structure is designed by using three-dimensional modeling software. After the solid model is generated, the assembly, meshing, and element attribute assignment are carried out by using finite element software. And the face-to-face contact relationship between each building is established, and the stress distribution of the exoskeleton is simulated and analyzed. The stress distribution of the exoskeleton under different working conditions is significantly different. Since the calculation does not take into account uncertainties such as shocks that may occur during walking, it is necessary to consider and multiply a certain safety factor when designing the optimized exoskeleton.