Skin

Expression and prognostic value of putative cancer stem cell markers CD117 and CD15 in choroidal and ciliary body melanoma

“…We can hypothesise that this might be due to the higher specificity and lower sensitivity of AEC staining, especially since all AEC-positive samples in our study were also positive when DAB chromogen was used. The use of red chromogen is advised in melanoma because the endogenous brown pigment may interfere with the detection of the more commonly used DAB chromogen 20 21. We did not use bleaching to eliminate melanin from the DAB stains to avoid any additional alterations in the expression of antigens.…”

“…Following peroxidase block, visualisation of the antigen–antibody complex was carried out with Dako EnVision FLEX system, using 3-amino-9-ethylcarbazole (AEC) and 3,3′-diaminobenzidine tetrahydrochloride (DAB) as chromogens. Two chromogen protocols were used because of possible interference of the endogenous pigment with DAB detection system in melanoma samples 20 21. Also, in order to preserve the epitopes, no bleaching was applied.…”