Skin and eye irritancy assessment of six lactic acid bacteria strains

Nikodinoska, Ivana; Spohr, Christina; Dillon, Gerald Patrick; Moran, C. A.

doi:10.1016/j.yrtph.2023.105406

Cited by 2 publications

(5 citation statements)

References 16 publications

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“…Several issues have been identified when discussing the utility of alternative studies for microbial pesticides including: soluble test material is needed for multiple assays; test materials are required to have a known concentration; potential interference of microbial products or large proteins with detection methods like fluorescence; and lack of true microbial positive or negative controls, which could help interpret study results. A promising outlook in direction of study applicability provides the data reported by Nikodinoska and coauthors [ 150 ]. They tested in vitro skin irritation assays (OECD 439 and a modified OECD 439) with six lactic acid bacteria strains.…”

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

“…Modifications of the main experiment according to OECD 439 [ 148 ] were made during the test item removal step. In the recovery phase the treated tissues were incubated in medium supplemented with 8–10 µg/mL streptomycin [ 150 ]. Data show that the test items are not suitable for the OECD 439 protocol, but may be suitable for the modified protocol.…”

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

“…As for the skin irritation also for eye irritation, Nikodinoska and coauthors provide an interesting initial investigation in the study of the applicability of in vitro assays for microbials [ 150 ]. The authors investigated the applicability of the RhCE test method (OECD 492) [ 158 ] and the in vitro Short Time Exposure In Vitro Test Method (OECD 491) [ 156 ] and the use of six lactic acid bacteria strains.…”

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

“…Data from Nikodinoska and coauthors [ 150 ] describe the scientific progress towards answering applicability aspects of in vitro assays for microbials. The appropriateness of non-animal tests for the evaluation of microbial pesticide serious eye damage may be further explored by engaging with stakeholders to validate the alternative studies and explore the utility of the GHS additivity method with microbials to determine whether these could be reliable alternatives to the traditional in vivo test.…”

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

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Microbial pesticides – challenges and future perspectives for testing and safety assessment with respect to human health

Wend,

Zorrilla,

Freimoser

et al. 2024

Environ Health

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Plant protection measures are necessary to prevent pests and diseases from attacking and destroying crop plants and to meet consumer demands for agricultural produce. In the last decades the use of chemical pesticides has largely increased. Farmers are looking for alternatives. Biopesticides should be considered a sustainable solution. They may be less toxic than chemical pesticides, be very specific to the target pest, decompose quickly, and be less likely to cause resistance. On the other hand, lower efficacy and higher costs are two disadvantages of many biopesticides. Biopesticides include macroorganisms, natural compounds and microorganisms. Microbial pesticides are the most widely used and studied class of biopesticides. The greatest difference between microbial and chemical pesticides is the ability of the former to potentially multiply in the environment and on the crop plant after application. The data requirements for the European Union and the United States Environmental Protection Agency are highlighted, as these regulatory processes are the most followed in regions where local regulations for biopesticide products are not available or vague. New Approach Methods already proposed or harmonized for chemical pesticides are presented and discussed with respect to their use in evaluating microbial pesticide formulations. Evaluating the microbials themselves is not as simple as using the same validated New Approach Methods as for synthetic pesticides. Therefore, the authors suggest considering New Approach Method strategies specifically for microbials and global harmonization with acceptability with the advancements of such approaches. Further discussion is needed and greatly appreciated by the experts.

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Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

Section: Challenges Associated With Data Requirements Endpointsmentioning

confidence: 99%

See 2 more Smart Citations

Microbial pesticides – challenges and future perspectives for testing and safety assessment with respect to human health

Wend,

Zorrilla,

Freimoser

et al. 2024

Environ Health

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“…Therefore, the aim of the present study was to identify effective and Their identity and safety information have been recently published (Nikodinoska et al, 2022a(Nikodinoska et al, , 2022b(Nikodinoska et al, , 2022c(Nikodinoska et al, , 2022dNikodinoska, Heikkinen, & Moran, 2023;Nikodinoska, Spohr, et al, 2023). Strains of both species were combined with each other at two different ratios, with or without the addition of MnSO 4 , resulting in 24 different silage additive treatments (Table 1).…”

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confidence: 99%

Lactic acid bacteria and manganese‐based additives for improved lucerne, perennial grass, and perennial grass‐white clover preservation as silage

Nikodinoska,

Wambacq,

Landschoot

et al. 2024

Grass and Forage Science

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Lactic acid bacteria (LAB) are commonly used as silage inoculants. Manganese sulphate (MnSO4) has been proposed to protect certain LAB during oxygen exposure and improve silage aerobic stability (ASTA). The aim of the present study was to assess the efficacy of inoculants in preservation of different forages. A short‐term screening trial investigated the effect of 24 additive treatments, compared to a negative control, on ensiling perennial grass in mini‐silos (2.75 L) for 7 d. The additive treatments comprised different ratios of Lactiplantibacillus plantarum [IMI 507026, IMI 507027, and IMI 507028] and Pediococcus pentacaceus [IMI 507024 and IMI 507025], with or without MnSO4. Target parameters, such as low levels of fresh matter loss, pH, acetic acid, ammonia‐nitrogen fraction (NH3–N/Total N), and high levels of lactic acid and ASTA, were considered for treatment selection. Eight treatments were selected based on a principal component analysis and hierarchical clustering of the target parameters. These selected treatments subsequently underwent a 100 d long‐term trial using lucerne, perennial grass, and perennial grass‐white clover forages. All silages treated with the eight additives exhibited an improved homolactic fermentation and a lower (p < .001) pH, acetic acid, and ethanol content than the control. Although DM losses and ASTA markedly improved in the treatments containing MnSO4 in the short‐term trial, these effects were not observed in the long‐term trial. These outcomes suggest that the selected additives can improve silage fermentation quality across a broad range of forages, but further studies are required to assess the impact of MnSO4 on ASTA.

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Skin and eye irritancy assessment of six lactic acid bacteria strains

Cited by 2 publications

References 16 publications

Microbial pesticides – challenges and future perspectives for testing and safety assessment with respect to human health

Microbial pesticides – challenges and future perspectives for testing and safety assessment with respect to human health

Lactic acid bacteria and manganese‐based additives for improved lucerne, perennial grass, and perennial grass‐white clover preservation as silage

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