Skin-associated lymphocytes in the peripheral blood of patients with atopic dermatitis: Signs of subset expansion and stimulation

Dworzak, Michael; Fröschl, Gertraud; Printz, Dieter; Fleischer, Christine; Pötschger, Ulrike; Fritsch, Gerhard; Gadner, Helmut; Emminger, W.

doi:10.1016/s0091-6749(99)70436-7

Cited by 38 publications

(31 citation statements)

References 25 publications

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“…Atopic donors tended to have more proliferating CLA+ cells, which is in concordance with previous reports showing higher HLA-DR and/or CD25 expression on, as well as higher spontaneous proliferation of, freshly isolated CLA+ cells [6, 15, 16]. …”

Section: Discussionsupporting

confidence: 92%

Expansion and Proliferation of Skin-Homing T Cells in Atopic Dermatitis as Assessed at the Single Cell Level

Jung

Schulz

Zachmann

et al. 2003

Int Arch Allergy Immunol

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Background: Sensitization to aeroallergens is a frequent event in patients with atopic dermatitis (AD). The role of allergen-specific T cells in the pathogenesis of AD is, however, not fully clear. A detailed immunological characterization of allergen-specific T cells able to migrate to inflamed skin might therefore be helpful in determining the relevance of allergen-specific T cells to clinical symptoms. Objective: To establish a simple assay to simultaneously monitor the expression of skin-related homing molecules and their proliferative response to an allergen on peripheral T cells. To evaluate whether this assay is able to identify AD patients in whom exposure to an allergen induces dermatitis. Methods: The expression of CLA, CD7 and CD4 was simultaneously measured by flow cytometry with the DNA content as a parameter of proliferation at the single cell level. House dust mite antigen (DerP) and tetanus toxoid (TT) were used as antigens. Results: CD4+ CLA+ CD7–, but not CLA– T cells from patients with AD proliferated and expanded following DerP stimulation. In contrast, TT stimulation resulted in proliferation of both, CLA+ and CLA– T cells from both, atopic and healthy donors. Conclusions: Single cell analysis revealed a predominant localization of allergen-reactive T cells within the CD7– CLA+ CD4+ T cell subset in AD. Thus, this simple assay not only confirms previously published results, but also extends our knowledge on the biology of CLA and CD7 cells in AD. Ultimately, this will be helpful in classifying those patients who might benefit from allergen avoidance therapies.

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Section: Discussionsupporting

confidence: 92%

Expansion and Proliferation of Skin-Homing T Cells in Atopic Dermatitis as Assessed at the Single Cell Level

Jung

Schulz

Zachmann

et al. 2003

Int Arch Allergy Immunol

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“…CLA is expressed by the vast majority of skin-homing T cells as well as by a small subset of circulating benign lymphocytes, especially in patients with chronic dermatitis (23)(24)(25)(26). CLA is a modified selectin ligand that mediates binding of a subset of circulating lymphocytes to dermal endothelium (27,28).…”

Section: Discussionmentioning

confidence: 99%

Degree of CD25 Expression in T-Cell Lymphoma Is Dependent on Tissue Site

Jones

Ibrahim

Patel

et al. 2004

Clinical Cancer Research

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Purpose: Using concurrent tumor samples from different anatomical sites, we compared expression of the therapeutic targets CD25 and CD30 in T-cell lymphoma (TCL).Experimental Design: We examined levels of CD25 and CD30 by flow cytometry in tumor cells from peripheral blood and lymph node in 13 cutaneous TCL patients and by immunohistochemistry in concurrent lymph node and skin biopsy specimens in 17 additional TCL cases, mostly mycosis fungoides. Tumor cell expression was correlated with patterns of expression in nonneoplastic lymphocytes in 14 reactive lymph node and 10 skin samples showing chronic dermatitis. Expression of CD25 and CD30 in all biopsy samples was compared with that of cutaneous lymphocyte antigen (CLA), a mediator of skin homing.Results: By flow cytometry, we noted significantly decreased expression of CD25 in lymph node compared with peripheral blood in 8 of 13 TCLs, with no changes in CD30 levels in 4 cases studied. Using immunohistochemistry, CD25 was strongly expressed in epidermotropic tumor cells in 13 of 17 (76%) TCL skin specimens but was decreased in the corresponding lymph node in 12 of these cases. CD30 was expressed at roughly equal intensity in tumor cells from both sites, except in 1 case. CLA showed a similar pattern to CD25, being expressed by tumor cells in 16 of 17 (94%) skin specimens, but was largely absent in tumor cells in the corresponding lymph node in 12 of these patients. In T cells from reactive lymph node biopsy specimens, CD25 was highly expressed only in dermatopathic lymphadenitis associated with transient skin rashes.Conclusions: We demonstrate in vivo that decreased levels of CD25 expression occur in TCL when it involves lymph node, similar to what is seen with CLA. This demonstrable variation related to anatomical localization has implications for the measurement of surface expression of CD25 and for understanding the response of patients with cutaneous TCL to interleukin 2 receptor-targeted immunotherapy.

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“…In several T cell-mediated skin diseases CLA is an important skin homing epitope for T cells. Patients with severe atopic dermatitis have a significantly expanded proportion of CLA + T cells [34,35] and expression of CLA among CD8 + T cells is significantly elevated in patients with psoriasis [36]. Our finding suggests that the migration of T cells to skin in systemic sclerosis does not reflect an increase of circulating skin-homing CD4 + cells.…”

Section: Discussionmentioning

confidence: 67%

a4b1+ and a4b7+ CD4+ T cell numbers increase and CLA+ CD4+ T cell numbers decrease in systemic sclerosis

Scala

Paganelli

Sampogna

et al. 2005

Clinical and Experimental Immunology

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Skin-associated lymphocytes in the peripheral blood of patients with atopic dermatitis: Signs of subset expansion and stimulation

Cited by 38 publications

References 25 publications

Expansion and Proliferation of Skin-Homing T Cells in Atopic Dermatitis as Assessed at the Single Cell Level

Expansion and Proliferation of Skin-Homing T Cells in Atopic Dermatitis as Assessed at the Single Cell Level

Degree of CD25 Expression in T-Cell Lymphoma Is Dependent on Tissue Site

a4b1+ and a4b7+ CD4+ T cell numbers increase and CLA+ CD4+ T cell numbers decrease in systemic sclerosis

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