Skin decontamination by low-temperature atmospheric pressure plasma jet and dielectric barrier discharge plasma

Non-mechanical procedures for removing caries-infected dentin are warranted in dentistry. We previously demonstrated the marked sterilization effect for direct irradiation of low-temperature plasma using dentin model infected with Streptococcus mutans. However it requires 180 s of intraoral plasma irradiation to eliminate bacteria. We alternatively investigated whether plasma-treated water (PTW), i.e., pure water exposed to plasma in an atmosphere, has a same bactericidal activity with the plasma irradiation. In the infected dentin model, the viable S. mutans counts recovered by bur at depth of 0.8-2.4 mm from the cavity floor were 10 4 -10 6 CFU/ round bur. After PTW application for only 10 s, the count was significantly decreased to below the detection limit (2.5 CFU/round bur) or 3.0±5.0 CFU/round bur. Since the bactericidal activity of PTW is rapidly deactivated at body temperature (37°C), PTW is likely to be biocompatible and holds significant potential for non-mechanical procedures for removing caries-infected dentin.

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“…1) 11,12) . However, there are few reports about its application for sterilization in the field of dentistry [13][14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

Tasaki

Ohshima

Usui

et al. 2017

Dental Materials Journal

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“…Non-thermal plasma was described to have antimicrobial activity and to play an important role in the killing of microorganisms (2)(3)(4)(5). These developments resulted in a new and independent field, plasma medicine, in which one promising approach is to promote wound closure by stimulation of the cells involved.…”

mentioning

confidence: 99%

“…Such plasma contains variable compositions of ultraviolet light, free electrons, and charged particles as well as ROS (e.g. HO ⅐ , O 2 . , O 3 , and H 2 O 2 ) and RNS (e.g.…”

mentioning

confidence: 99%

Non-thermal Plasma Activates Human Keratinocytes by Stimulation of Antioxidant and Phase II Pathways

Schmidt

Dietrich

Steuer

et al. 2015

Journal of Biological Chemistry

129

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Background: Non-thermal plasma provides an interesting therapeutic opportunity to control redox-based processes, e.g. wound healing. Results: The transcription factor NRF2 and downstream signaling molecules were found to act as key controllers orchestrating the cellular response. Conclusions: Plasma triggers hormesis-like processes in keratinocytes. Significance: These findings facilitate the understanding of plasma-tissue interaction and its deduced clinical application.

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“…CAP technologies have been used effectively to eliminate microorganisms on living tissues in several clinical applications [8,80,[94][95][96][97][98][99][100][101] , including inactivation and eradication of fungi, and vegetative and spore-forming bacteria. [86,89,102] The microbial inactivation effect of CAP can be attained by direct exposure to both the ignited plasma discharge products such as ROS, RNS, UV radiation, or indirectly through the long-lived species that are capable to reach the treated substrate without the electric field effect [91] .…”

Section: Mechanisms Of Microbial Inactivation By Capmentioning

confidence: 99%

“…[110] Notably, CAP is also effective on the sterilization resistant Deinococcus radiodurans bacteria. [112] Less resistant vegetative bacteria have also been tested against the antimicrobial activity of CAP, and were eliminated with different degrees of survival, including gramnegative: Escherichia coli [111,113,114] , Salmonella typhimuriu [115] , and gram-positive Staphylocuccus epidermidis [90,113,116] , Staphylococcus aureus [90,114] , Micrococcus luteus [95] , Streptococcus pyogenes [90] , Enterococcus faecalis [117] and Enterococcus faecium. [90] Gramnegative bacteria are more susceptible to gas plasma treatments than gram-positive [104,118] and differences in susceptibility are thought to be due to variation in the thickness of the peptidoglycan murein layer in the bacterial cell wall.…”

Section: Mechanisms Of Microbial Inactivation By Capmentioning

confidence: 99%

Terminal sterilization: Conventional methods versus emerging cold atmospheric pressure plasma technology for non‐viable biological tissues

Marsit

Sidney

Branch

et al. 2016

Plasma Processes & Polymers

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Tissue products are susceptible to microbial contamination from different sources, which may cause disease transmission upon transplantation. Terminal sterilization using gamma radiation, electron‐beam, and ethylene oxide protocols are well‐established and accepted, however, such methods have known disadvantages associated with compromised tissue integrity, functionality, safety, complex logistics, availability, and cost. Non‐thermal (cold) atmospheric pressure plasma (CAP) is an emerging technology that has several biomedical applications including sterilization of tissues, and the potential to surpass current terminal sterilization techniques. This review discusses the limitations of conventional terminal sterilization technologies for biological materials, and highlights the benefits of utilizing CAP.

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Skin decontamination by low-temperature atmospheric pressure plasma jet and dielectric barrier discharge plasma

Cited by 182 publications

References 21 publications

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

Plasma-treated water eliminates <i>Streptococcus mutans</i> in infected dentin model

Non-thermal Plasma Activates Human Keratinocytes by Stimulation of Antioxidant and Phase II Pathways

Terminal sterilization: Conventional methods versus emerging cold atmospheric pressure plasma technology for non‐viable biological tissues

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